Proteomics Service Center

The Proteomics Service Center provides label-free and multiplexed proteomics workflows for both BU investigators and the external research community. Its research faculty have extensive experience in proteomics analysis of tissue specimens and tissue slides. The center offers spatial proteomics using a Leica LMD6 laser microdissection microscope and has significant expertise in TMT multiplexed deep proteomics, phosphoproteomics, affinity proteomics, and proximity labeling proteomics. Additionally, it provides mass spectrometry-based glycoproteomics and glycomics analyses. It operates two Thermo Fisher Exploris 480 LC-MS systems and offers a comprehensive set of bioinformatics services.

To meet the growing demand for tailored mass spectrometry applications across BU campuses, the Proteomics Service Center has partnered with the BU Target Discovery and Proteomics Lab (BU-TDPL) to strategically triage experiment requests to ensure they are matched with “the best instrument for the job.” The TDPL harbors a Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer, which offers specialized capabilities such as High Mass Range MSn (HMRn), Proton Transfer Charge Reduction (PTCR), and a variety of dissociation techniques, including CID, HCD, ETD, EThcD, and UVPD, providing a comprehensive range of options for intact protein analysis. The TDPL also specializes in workflows for discovering small molecule-protein interactions, including the Proteome Integral Solubility Alteration (PISA) assay, biotinylated probe pulldowns, and more.