• GMS BI 777: Techniques in Biomedical Research
    Graduate Prerequisites: consent of instructor
    Success in biomedical research requires proposing, developing and testing a novel hypothesis. The generation of a novel hypothesis in turn requires the ability to apply the scientific method and then implement the appropriate techniques to address the experimental question. This course will complement the Foundations in Biomedical Sciences (FiBS) curriculum by providing students with a comprehensive understanding of the core experimental methods used in biomedical research. By the end of this course, students will master the concepts behind a wide range of experimental techniques and technologies and then be prepared to apply the most appropriate experimental system to a given biological question. Biochemical knowledge regarding "how things work" and "how to cook from scratch in the lab" will enable students to develop their own experimental research strategies. Specific topics to be covered include: the scientific method/lab basics, cell culture and gene transfer, protein extraction and analysis, DNA and cloning, PCR, DNA-protein interactions and chromatin, RNA and quantitative PCR, lipids, transgenic and knockout mice, mass spectrometry, flow cytometry, microarray and next generation sequencing, histology and confocal microscopy. This course is team taught and will use lectures, in class discussions, and focused problem sets. A concise final written assignment is designed to test the students' mastery of the subject matter. 2 cr., Fall sem.
  • GMS BI 778: Molecular Mechanisms of Cardiovascular Disease
    The course deals with research topics relevant to cardiovascular disease including lipoproteins, atherosclerosis, oxidative stress, diabetes, hypertension, congenital heart abnormalities, gene therapy, stem cell therapies and others. Emphasis is placed on molecular and cellular mechanisms of normal vascular function and of vascular dysfunction leading to disease. Each session is taught by an expert in the field. Each student presents an original paper assigned by the instructors and writes and presents a review. Offered alternate years. 2 cr, Fall sem.
  • GMS BI 793: Mass Spectrometry, Proteomics and Functional Genomics
    Graduate Prerequisites: consent of instructor
    The application of mass spectrometry to protein, glycoconjugate and carbohydrate structures has propelled developments in proteomics and functional genomics. This course describes how to use mass spectrometry to answer structural and functional questions in biomedical research. The course explores the background necessary to effectively design mass spectrometric (MS) experiments and interpret data. Students gain a full understanding of modern MS and its effective use in research. Lectures are devoted to instrumentation, ionization methods, applications to proteins, lipids, carbohydrates, glycoconjugates, nucleic acids and uses of the technology in proteomics, biotechnology and medicine. 2 cr, Spring sem.