Cheng Lin

A major focus of my current research lies in the development of an integrated analytical and bioinformatics approach for high-throughput de novo glycan sequencing. We utilize electron activated dissociation (ExD) methods to generate structurally informative tandem mass spectra, both off-line and on-line with various glycan separation methods, including high-performance liquid chromatography (HPLC) and ion mobility spectrometry (IMS) for characterization of complex glycan mixtures. We also carry out theoretical studies of the glycan ExD fragmentation processes, and collaborate with bioinformaticians to develop software tools for spectral interpretation and structural determination.

Another area of my research focuses on direct MS analysis of protein acylation. Reversible S-acylation modulates the protein hydrophobicity and plays an important regulatory role in protein trafficking, membrane localization and cellular signaling. We are developing sample preparation and LC-MS/MS methods for enrichment, preservation and reliable characterization of protein acylation.