Appendix J: Summary of Requirements for Animal Biosafety Levels
Safety Guideline ABSL1 ABSL2 ABSL3 ABSL4 Access is limited or restricted at the discretion of the laboratory for satellite laboratories or the Attending Veterinarian for all laboratories. Y Y Y Y Personnel must wash their hands after handling cultures and animals, removing gloves, and before leaving the facility. Y Y Y Y Eating, drinking, and […]
Appendix K: Bloodborne Pathogen Standard
Avoiding occupational exposure to human blood, body fluids, and tissues is the primary way to prevent transmission of bloodborne pathogens. The goal of the initial and annual standard precautions training is to present information on how to prevent such exposures by administrative controls, workplace engineering controls, proper work practices, personal protective equipment, and a hepatitis […]
Appendix L: Working Safely with Animals
Working with animals poses potential additional health and safety hazards that require extra precautions. The specific requirements will depend on the types of activities (e.g., surgery, feeding animals, use of anesthetic agents, etc.) and the specific species used. Personnel Guidelines Personnel should follow the following guidelines: Follow the specific requirements established in the IACUC-approved protocol […]
Appendix M: Procedures for Working in an Animal Biosafety Level 2 (ABSL2) Facility at BU
Before starting any Animal Biosafety Level 2 (ABSL2) work a PI must: Obtain IBC and IACUC approval Make appropriate housing arrangements with the ASC director The following Standard Operating Procedures (SOP) have been developed to provide guidance to those individuals working in rooms in which animals involved in chemical and biological hazards determined to be […]
Appendix N: Verification of Attenuated Biosafety Level 3 (BSL3) Pathogens Policy
Purpose The purpose of this policy is to set forth the procedures for verification of attenuated BSL3 pathogens before they may be safely handled at the lower biosafety level (BSL) designated for the attenuated pathogen. Covered Parties This policy applies to all individuals engaged in research at or under the auspices of Boston University (BU) […]
Appendix O: Boston Public Health Commission Requirements
The Biological Laboratory Regulations adopted by the Boston Public Health Commission (BPHC) as amended in October 2019 requires laboratories working with rDNA and those at Biosafety Level 3 (BSL3) and Biosafety Level 4 (BSL4) to adhere to local standards, including: Obtaining an annual Recombinant DNA permit from the BPHC. An IBC that reports to a […]
Appendix Q: Laboratory and Equipment Decontamination Procedures
Decontamination of Lab Space and Equipment Punch List Assigning a designate a Move Coordinator. The coordinator will work with EHS and other stakeholders to facilitate and coordinate the move. Contact an outside vendor for the decontamination of biological safety cabinets (tissue culture hoods). Have appropriate personal protective equipment available (lab coat, gloves, eye protection). Dispose […]
Appendix T: Boston University Research Compliance Policy on Disease Surveillance and Reporting for High-Risk Agents
Purpose and Applicability This policy implements BPHC’s Guidelines for Implementation and Enforcement of Boston Public Health Commission’s Disease Surveillance and Reporting Regulation. The BPHC’s guidelines require laboratory registration and a medical surveillance program for research laboratories working with high-risk agents. The guidelines are designed to ensure that BPHC receives timely access to information regarding incidence […]
Appendix U: Criteria for Development of Standard Operating Procedures (SOP)
In this manual, there are a number of sections where the laboratory is required to prepare standard operating procedures (SOPs). This appendix is intended to provide guidelines on the development of such documents. It is not mandatory to follow these procedures. The lab may seek the advice of EHS when developing an SOP. Introduction What […]
Chapter 9: Biohazardous and Medical Waste Disposal
In the Commonwealth of Massachusetts, biohazardous waste is governed by the Department of Public Health regulation 105 CMR 480, “Storage and Disposal of Infectious or Physically Dangerous Medical or Biological Waste, State Sanitary Code Chapter VIII.” Boston University has biological waste management guidance documents for each campus which are reviewed annually and posted on the […]