| Safety Guideline | BSL1 | BSL2 | BSL3 | BSL4 |
|---|
| Laboratory personnel must wash their hands after handling cultures, removing gloves, and before leaving the laboratory. | Y | Y | Y | Y |
| Eating, drinking, and application of cosmetics is prohibited. | Y | Y | Y | Y |
| Personnel must be familiar with basic biosafety procedures, including this manual. | Y | Y | Y | Y |
| Personnel should wear face protection, such as goggles, face shields or other device providing protection against any possible splashes and aerosols. | Y | Y | Y | Y |
| Pipetting by mouth is prohibited. | Y | Y | Y | Y |
| All laboratory procedures should be performed to minimize aerosol generation. | Y | Y | Y | Y |
| Work surfaces must be decontaminated at least daily, after each use for infrequent users, and after any spill of viable materials. | Y | Y | Y | Y |
| Sharps must be placed in specially designed puncture- and leak-proof sharps containers and disposed of appropriately as medical waste. | Y | Y | Y | Y |
| Laboratories must be kept neat; good housekeeping procedures must be in place and in regular use. | Y | Y | Y | Y |
| All medical waste is decontaminated before disposal by an approved decontamination method and/or disposed of as medical waste. | Y | Y | Y | Y |
| Insect and rodent control programs are instituted. | Y | Y | Y | Y |
| Laboratory contains a sink for handwashing. | Y | Y | Y | Y |
| Laboratories are designed for ease of decontamination (e.g., no carpets, sealed surfaces, no unreachable areas, etc.). | Y | Y | Y | Y |
| Bench tops are impervious to water, moderate heat, and chemicals. | Y | Y | Y | Y |
| Laboratory furniture must be secured, and spaces between benches, cabinets, and equipment must be accessible for decontamination. | Y | Y | Y | Y |
| All laboratory windows must be fitted with fly screens. | Y | Y | Y | Y |
| Laboratory coats or gowns and gloves must be worn. | Y | Y | Y | Y |
| Autoclaves are required for waste treatment prior to disposal -biohazardous waste. | N | N | Y | Y |
| Autoclave quality control program is required for use specified above. | Y | Y | Y | Y |
| Instructions for safety precautions are posted by the Principal Investigator. | Y | Y | Y | Y |
| Animals not involved in the experiment are not permitted in laboratory. | N | Y | Y | Y |
| Biological safety cabinets are required and must be certified annually. | N | Y | Y | Y |
| Laboratory personnel require specific training in the handling of pathogenic materials. | N | Y | Y | Y |
| Safety centrifuge cups are required. | N | Y | Y | Y |
| Access to facility is limited or restricted during experiments. | N | Y | Y | Y |
| The universal biohazard symbol must be posted on the access door to the laboratory. | N | Y | Y | Y |
| Immunization and/or serological testing for agents to be handled may be required. | N | Y | Y | Y |
| All laboratory procedures must be performed in a properly certified biological safety cabinet. | N | N | Y | Y |
| Laboratory requires controlled entry, unidirectional air flow, and other special design features. | N | N | Y | Y |
| Windows must be closed and sealed. | N | N | Y | Y |
| No material or equipment can leave the laboratory unless it is autoclaved or decontaminated. | N | N | Y | Y |
| Autoclaves must be located inside the laboratory. | N | N | Y | Y |
| Access is through an airlock system. | N | N | N | Y |