| Safety Guideline | ABSL1 | ABSL2 | ABSL3 | ABSL4 |
|---|
| Access is limited or restricted at the discretion of the laboratory for satellite laboratories or the Attending Veterinarian for all laboratories. | Y | Y | Y | Y |
| Personnel must wash their hands after handling cultures and animals, removing gloves, and before leaving the facility. | Y | Y | Y | Y |
| Eating, drinking, and application of cosmetics is prohibited. | Y | Y | Y | Y |
| Personnel must be familiar with basic biosafety procedures, including this manual. | Y | Y | Y | Y |
| Personnel should wear goggles or face shields if the possibility of splashes and aerosols exists. | Y | Y | Y | Y |
| Pipetting by mouth is prohibited. | Y | Y | Y | Y |
| All procedures should be performed to minimize aerosol generation. | Y | Y | Y | Y |
| Work surfaces must be decontaminated at least daily with an approved disinfectant, after each use for infrequent users, and after any spill of viable materials. | Y | Y | Y | Y |
| Sharps must be placed in specially designed puncture- and leak-proof sharps containers and disposed of appropriately as medical waste. | Y | Y | Y | Y |
| Facilities must be kept neat; good housekeeping procedures must be in place and in regular use. | Y | Y | Y | Y |
| All medical waste is decontaminated before disposal by an approved decontamination method and/or disposed of as medical waste. | Y | Y | Y | Y |
| Insect and rodent control programs are instituted. | Y | Y | Y | Y |
| Doors to animal rooms are kept closed when experimental animals are present. | Y | Y | Y | Y |
| Facilities are designed for ease of decontamination (e.g., no carpets, sealed surfaces, no unreachable areas, etc.). | Y | Y | Y | Y |
| Bedding materials from animal cages are removed in a manner that minimizes aerosol production and are disposed of as medical waste. | Y | Y | Y | Y |
| Instructions for safety precautions are posted by the Principal Investigator. | Y | Y | Y | Y |
| Facility windows and vents that open must be fitted with fly screens. | Y | Y | Y | Y |
| Laboratory coats or gowns and gloves must be worn. | Y | Y | Y | Y |
| Autoclaves are required for waste treatment prior to disposal as biohazardous waste. | N | Y | Y | Y |
| Autoclave quality control program is required for use specified above. | Y | Y | Y | Y |
| Cages are washed prior to release or reuse. | Y | Y | Y | Y |
| Air is exhausted to the outside without recirculation. | N | Y | Y | Y |
| Personnel baseline serum samples may be required. | N | Y | Y | Y |
| Facility personnel require specific training in the handling of pathogenic materials. | N | Y | Y | Y |
| Safety centrifuge cups are required. | N | Y | Y | Y |
| Access to facility is limited or restricted during experiments. | N | Y | Y | Y |
| The universal biohazard symbol must be posted on the access door to the facility. | N | Y | Y | Y |
| Immunization and/or serological testing for agents to be handled may be required. | N | Y | Y | Y |
| All procedures must be performed in a properly certified biological safety cabinet. | N | N | Y | Y |
| Facility requires controlled entry, unidirectional air flow, and other special design features. | N | N | Y | Y |
| Windows must be closed and sealed. | N | N | Y | Y |
| No material or equipment can leave the facility unless it is autoclaved or decontaminated. | N | N | Y | Y |
| Autoclaves must be located inside the facility. | N | N | Y | Y |
| Access is through an airlock system. | N | N | N | Y |