Laboratory Safety – Programs & Services

When working with a material or equipment for the first time, EHS recommends that you familiarize yourself with hazards that exist before beginning any tasks. Take these steps to understand these hazards you may encounter: ²

1. 1. Review the safety section of equipment and machinery manuals.
   2. Read Section 2 of the chemical safety data sheets (SDSs) used in your lab or workplace.
   3. Read the door placard before entering a laboratory (see figure below).
   4. Look for signage in lab or around machinery (see figure below).
   5. Research patterns or incidents of near misses, injuries or illness that have occurred in your workplace with materials or equipment that you are using.
   6. Communicate with current and former colleagues that are familiar with your operation.
   7. Reach out to EHS to perform a procedural risk assessment.

Once hazards have been identified, you, in collaboration with your colleagues, supervisor, and EHS, can identify PPE that will most adequately protect you. There are many types of PPE which can be used to protect from various maladies or routes of entry. Each type of PPE may have different makes or models, each with their own limitations such as service life or assigned protection factors (APF). APF helps determine if PPE will protect you based on the frequency, duration of work, and concentration of materials.

Examples of PPE: 

For more information about respiratory protection visit this EHS Respiratory Protection Page.

 

Certain PPE requires special attention when putting it on (donning) and taking it off (doffing). When donning and doffing PPE, the sequence of events is very important to reduce the likelihood of exposure to the unprotected parts of your body, especially in a laboratory setting. Generally, the order is as follows ³:

Donning	Doffing
1.     First layer of gloves (if double gloving)*	1.     First layer of gloves (if you are double gloved)*
2.     Gown or lab coat	2.     Lab goggles or safety glasses
3.     Face mask or respirator (if any)	3.     Gown or lab coat
4.     Lab goggles or safety glasses	4.     Face mask or respirator (if any)
5.     Last layer of gloves (if double gloving)	5.     Last layer of gloves
*If double gloving is not part of your procedure, skip this step

When doffing disposable gloves, it is important to follow a specific technique to reduce probability of transferring hazardous materials to your bare hands. See the diagram below for guidance ⁴.

Video on Donning and Doffing Gloves

PPE will not protect you adequately unless it is used and maintained as the manufacturer intended. Please keep the following in mind when using PPE:

1. 1. Disposable gloves are SINGLE USE ONLY. Furthermore, if gloves break during your work, doff them correctly (see section above), wash your hands and don a new pair.
   2. Remove disposable gloves before reaching for commonly touched items. Items such as elevator buttons, stair railings and door handles should not be touched with a gloved hand. Always doff disposable gloves before exiting the laboratory.
   3. Do not wash body PPE with street clothing. Residual materials may stay on your street clothes when laundered with your body PPE. This could inadvertently expose your family and friends.
   4. If you can avoid it, do not bring PPE home. By keeping your PPE at work, you will reduce the likelihood of exposing your family and friends to dangerous materials.
   5. Always have spare PPE ready to use. Broken safety glasses or torn gloves are equivalent to not wearing protection at all. Never hesitate to throw out damaged or soiled PPE and get a new set.
   6. Inspect PPE for broken parts, rips, or holes. It can be hard to see damaged PPE during donning. Make sure you inspect your PPE one time before you enter a contaminated space or begin work.
   7. Make sure PPE is the correct fit. Similar to broken PPE, if the sleeves of your lab coat are too short or your nitrile gloves are too big, for example, your skin may be exposed, making you more susceptible to injury or illness.
   8. Store PPE in an area that will preserve it in good condition. Read the manufacturer instructions on how to store PPE. Keeping disposable gloves in a hot car or windowsill instead of a cabinet, for example, will degrade the nitrile/rubber and increase the likelihood of breakage during use.

• BU Facilities Management & Planning PPE Training Handout
• NIOSH Chemical Protection Clothing Database
• OSHA Assessing the Need for PPE Document
• OSHA Face and Eye Protection eTool
• OSHA PPE Website
• OSHA Publication 1351-12R - PPE
• U.S. Fish and Wildlife Service - Glove Selection Guide

1. “Hierarchy of Controls.” Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, 13 Jan. 2015, https://www.cdc.gov/niosh/topics/hierarchy/default.html
2. “Recommended Practices for Safety and Health Programs” Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, 28 Sept. 2020, https://www.osha.gov/shpguidelines/hazard-prevention.html#ai1
3. “Sequence for Donning and Doffing Personal Protective Equipment (PPE)” Centers for Disease Control and Prevention, Centers for Disease Control and Prevention, 28 Sept. 2020, https://www.cdc.gov/hai/pdfs/ppe/ppeposter148.pdf.
4. “Glove Removal Procedure” WorkSafeBC, Workers Compensation Board of B.C., 24 November 2020, https://www.worksafebc.com/en/resources/health-safety/ppe-information-sheets/glove-removal-procedure?lang=en