Inactivated Biological Sample Use
The purpose of the Inactivated Biological Samples Use policy is to develop a process whereby samples generated as BSL-3 and BSL-4 can be used safely at lower biosafety levels. Inactivation of biological samples renders them non-infectious and therefore they can be handled as Risk Group 1 (RG1) agent under BSL-1 conditions. This policy requires that […]
Section 2: ALARA Policy
Updated May 2020 As Low as Reasonably Achievable (ALARA) Policy BU and BMC are committed to maintaining exposures As Low as Reasonably Achievable (ALARA). Although current occupational radiation exposure limits present a very low risk of harm, it is prudent to practice radiation safety techniques and protocols to minimize unnecessary exposures. The goal of an […]
Section 1: Introduction
Updated May 2020 Introduction The use of radioactive materials is strictly regulated by federal, state and local agencies to ensure the safety of radiation users and the public. It is important for all individuals within BU/BMC who use radioactive materials to read and abide by the rules and practices documented within this manual. Radioactive materials […]
Chapter 7: Decontamination and Sterilization
Definitions Decontamination is a process or treatment that renders a device, instrument, or work surface safe to handle. A decontamination procedure can range from sterilization by autoclave or ethylene oxide to simple cleaning with soap and water. Sterilization, disinfection, and antisepsis are all forms of decontamination. Sterilization is the use of a physical or chemical […]
Appendix I: Summary of Requirements for Biosafety Levels
Safety Guideline BSL1 BSL2 BSL3 BSL4 Laboratory personnel must wash their hands after handling cultures, removing gloves, and before leaving the laboratory. Y Y Y Y Eating, drinking, and application of cosmetics is prohibited. Y Y Y Y Personnel must be familiar with basic biosafety procedures, including this manual. Y Y Y Y Personnel should […]
Appendix H: Prion Research/Creutzfeldt-Jacob Disease (CJD) Guidelines
Creutzfeldt-Jacob Disease (CJD) is one of a group of neurodegenerative diseases called transmissible spongiform encephalopathies (TSE) (or prion diseases) which affect humans (e.g., Kuru, Fatal Familial Insomnia and Gerstmann-Sträussler-Scheinker syndrome of humans) and a variety of domestic and wild animal species (e.g., Scrapie of sheep, “Bovine Spongiform Encephalopathy [BSE] or Mad Cow Disease” of cattle […]
Appendix E: Biosafety Level 3 (BSL3) and Biosafety Level 4 (BSL4) Requirements and Practices
Biosafety Level 3 and 4 (BSL3/4) is the recommended containment for work with agents or toxins that may cause serious or potentially fatal disease through inhalation exposure. Work at BSL3/4 requires enhanced facility design, operational controls and special practices, which are outlined in the BSL3 Biosafety Manual and BSL4 Biosafety Manual. BSL3/4 facilities are designed […]
Appendix J: Summary of Requirements for Animal Biosafety Levels
Safety Guideline ABSL1 ABSL2 ABSL3 ABSL4 Access is limited or restricted at the discretion of the laboratory for satellite laboratories or the Attending Veterinarian for all laboratories. Y Y Y Y Personnel must wash their hands after handling cultures and animals, removing gloves, and before leaving the facility. Y Y Y Y Eating, drinking, and […]
Appendix K: Bloodborne Pathogen Standard
Avoiding occupational exposure to human blood, body fluids, and tissues is the primary way to prevent transmission of bloodborne pathogens. The goal of the initial and annual standard precautions training is to present information on how to prevent such exposures by administrative controls, workplace engineering controls, proper work practices, personal protective equipment, and a hepatitis […]
Appendix L: Working Safely with Animals
Working with animals poses potential additional health and safety hazards that require extra precautions. The specific requirements will depend on the types of activities (e.g., surgery, feeding animals, use of anesthetic agents, etc.) and the specific species used. Personnel Guidelines Personnel should follow the following guidelines: Follow the specific requirements established in the IACUC-approved protocol […]