Cage Card Notification System

White in color with unique number and barcode for tracking. The BUASC will issue completed cage cards once the animals are ordered and received by a vendor. Lab specific cage cards can be found in each lab’s animal holding room and can be used when the researcher or BUASC establishes a new cage of animals by weaning or separation. This card has two perforated strips on the top; one for activation of card and second for deactivation of card. All cages must have a primary cage card with the following information and conform to BUASC format:

• PI Name
• Financial Account
• Protocol (PROTO) number
• User’s name
• Phone number
• Species
• Sex/Age/Weight
• Strain
• DOB
• Vendor/IHB
• Arrival date (if applicable)

Learn more about filling out Primary Cage Cards

Teal in color, this card is used to designate cages moving to another room/building within BU, transferring to another protocol and/or another PI, or transporting out to another institution. This card must be placed by the research staff for any transfers/transports within BUASC maintained colonies. All transfers/transports must first be approved by BUASC by submission request in AOPs. Physical movement of cages between rooms and/or changing of protocols on cards without permission is prohibited. BUASC staff will complete all transfers/transports once request is approved. When completing a transfer/transport, BUASC will update cage cards accordingly to reflect change.

Learn more about the transfer process and filling out Transfer/Transport Cards

Green in color, this card is used to flag any non-emergency concern (i.e. overcrowded cage, wean due, missing information on cage card, etc.). Noteworthy: this card does not include non-emergency health concerns (see Vet Services card). The lab must rectify noted problems within reasonable timeline with the exception of overcrowded cages. The lab is allowed up to 48 hours to correct an overcrowded cage unless an emergency arises (double litters, fighting animals, etc.). It is the responsibility of the lab to maintain colony breeding and weaning. BUASC assistance in aforementioned cases are chargeable services. In emergency events, BUASC will separate animals immediately upon finding. The veterinarian will be consulted for permission to supply standard chow and water for any cages that have been observed to have very little (2-3 pellets) or no special food or water.

Red in color, this card is used to indicate that an animal within the cage has an active health concern. When a health concern is noted, the animal(s) are under the care of Veterinary Services. Presence of this card on the cage means the lab should also have received communications via Health Concern email. This card is used in conjunction with a health case monitoring form for BUASC to document each case on a daily/weekly/monthly schedule.

Beige in color, this card is placed by ASC staff when single housed animals are noted. At the next cage change, additional enrichment will be given to any cages that are not marked for enrichment restriction. The mice will receive extra enrichment during cage change until the animal is euthanized or paired (follow male group housing policy).

Purple in color, this card is used to flag newly arrived animals to the facility from BU approved animal vendors. This card is placed by BUASC staff upon housing the animals and will stay on during their acclimation period. BUASC will monitor these cages closely during this time and remove the card approximately 1 week later.

This card is used for lab staff to communicate to BUASC staff when any animal manipulations were completed within the cage. This card is to be filled out in it is entirely. This card also allows the BUASC staff to look for any abnormal post-procedure behavior once returned to the housing room. A post-procedure monitoring form for rodents should be filled out after a surgical procedure by the researcher to document observations.

This card is placed at the cage level by BUASC when informed by the IACUC that a protocol is on HOLD (reason for this varies and questions should be directed to IACUC). The animals are placed on a BUASC holding protocol and the lab is restricted from any manipulation or handling (including but not limited to weaning, setting up breeding pairs, collecting weights, etc.) of these animals while the protocol is on Hold. Once cleared by IACUC to remove from holding, BUASC will remove the cards.

Yellow in color, these cards are used to indicate any special care within the cage that differs from normal husbandry. This includes special food/water, fasting, special bedding, extra enrichment, etc. It should be noted whether the lab or BUASC staff will provide care of special requirements when applicable. The researcher should contact BUASC manager to find out what special items the BUASC can do. Please note that BUASC completion of special instructions may incur a charge to the lab for the service. The entirety of the card should be filled out, especially a contact number for the individual making the request.

Yellow in color, this card is used to indicate special care within the cage that differs from normal husbandry, specifically the restriction of food or water as it pertains to approved experiments in their protocol. This card is placed after a Special Care Request Form is submitted to ASC for approval to start restriction. Learn more about Special Care Requests here. The card should state whether the lab and/or BUASC staff will provide care. Please note that BUASC completion of special instructions may incur a charge to the lab for the service. This card is used in conjunction with a “color coded dot system” to standardize information and minimize manual writing; dots are to be placed in corresponding boxes on the card for the care requested.

The determination of the minimum caloric requirement or minimum fluid required to maintain the health of each animal is required prior to starting food or fluid restriction studies. Learn more about Food and Fluid Regulation and Restriction (IACUC) here.

• RED Dot = Food Restriction: Number of pellets to provide the cage is written on the dot.
• BLUE Dot = Timed Feeding: Number of pellets to provide cage is written on the dot and provided at specified time of day (time determined by the lab).
• GREEN Dot = Restricted Water: Water is provided to cage via dish. Learn more about rodent fluid regulation here.
• YELLOW Dot = Used in tandem with a GREEN dot: Denotes ASC staff will provide or remove water access on designated day (s) and time (s) as specified by the lab.

Pink in color, this card is placed by the BUASC staff when the observation of a new litter is present in the cage. This card can be placed by the research staff if they spot the litter first. It contains the birth date (DOB) and wean date of the new litter. The wean date is 21 days from the date of birth. Mice and rats can deliver litters every 21 days. If 2 litters (otherwise known as double litters) are present in a cage, the older pups must be immediately separated out (overcrowding; referred to as emergency wean) unless otherwise approved on the IACUC protocol or by vet services due to a health concern evaluation. It is the responsibility of the lab staff to keep track of the breeding colony and weaning events to help reduce charges to their lab. Any cage not weaned out within 48 hours of the weaning date will be weaned by the BUASC for a fee (as noted in the special service log). In addition, any double litters found by BUASC will be separated and also constitutes as a chargeable service.

Pink in color, this card is placed by research staff when setting up new mating pairs. The card contains information on parent strain, DOB, and ID if any. The litter production is also tracked on the card by ASC if we initiate any separation of the litter.

Learn more about breeding animals and filling out Breeder Cards