Prereq: GMS BT110 and GMS BT208. This course will focus on the fundamental laboratory skills that students need to prepare for a career in the biomedical sciences. The course emphasizes the theoretical as well as the applied aspects of basic methodologies in research. The course is reinforced with applied, hands-on laboratory sessions that provides practical experience in the topics covered in the preceding lectures. The majority of class time will be designed to provide students with ample hands-on time in the lab to practice their skills in the presence of the course instructor. The course will focus on providing students with a set of basic laboratory skills, such as safety practices, laboratory mathematics, documentation, and good laboratory ethics. Students will also be educated in research methodology, data analysis, and data presentation. Topics covered include solution chemistry; protein extraction and detection using Western blot and ELISA; the basic principles of immunohistochemistry; cell culture basics, and RNA extraction. Students, even those with some previous laboratory hands-on experience, will benefit from this course because of its combined focus on the theoretical and applied aspects of laboratory techniques/methods. The proposed topics of study would include: Introduction to Proteins Protein Detection Methods; Gene Expression; Principles of Immunohistochemistry and Protein Colonization; Microscopy and Imaging, etc.

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