Data Link 5
The table below has additional primary references for Table 1 in Wang & Gilmore, 2002., Biochimica et Biophysica Acta – Molecular Cell Research
Table 1 Interaction partners of zyxin/paxillin family proteins
| Protein | Cytoskeletal and/or Plasma Membrane |
Signaling Molecules |
Transcription Factors/ Nuclear Proteins |
Others |
| Zyxin | a-actinin [34-36] | Vav [41] | HPV E6 protein [22] | |
| Mena/VASP [37,38] | CasL [31] | SON DNA-binding protein [31] |
||
| CRP [39] | p130Cas [31] | |||
| H-warts/LATS1[40] | ||||
| Trip6 | OpaP [42] | Grb2 [79] | Thyroid hormone receptor [15] |
RIL [44] |
| Tropomyosin 4 [31] | PTP-BL/1E [44,45] | Retinoid X receptor [15] | Novel protein [31] | |
| CasL [31] | v-Rel [17] | |||
| p130Cas [31] | SON DNA-binding protein [31] |
|||
| gp210 [31] | Atrophin-1 related protein [31] |
|||
| Synaptic GTPase Activating Protein [31] |
||||
| LPP | Mena/VASP [8] | |||
| Ajuba | Grb2 [45] | TTF1 [19] | ||
| GLT1 [46] | ||||
| Paxillin | b1, b3, a4, and a6 subunits [47-50] |
FAK [54,56,58] | BPV E6 protein [32,33] | Poly(a)-binding protein 1 [66] |
| syndesmos [51] | CAKb/Pyk2 [58,59] | |||
| tubulin [52] | ILK [60] | |||
| vinculin [53-56] | Src [61] | |||
| actopaxin [57] | PKL [62] | |||
| schwannomin [77] | PAK [62,64] | |||
| Csk [65] | ||||
| Crk [66] | ||||
| PTP-PEST [67] | ||||
| Hic-5 | vinculin [69] | FAK [69-71] | Androgen receptor [18] |
|
| syndesmos [51] | CAKb/Pyk2 [72,73] | Glucocorticoid receptor [5] |
||
| actopaxin [57] | PKL [63] | |||
| dopamine transporter [78] |
Csk [73] | |||
| PTP-PEST [74] | ||||
| GLT1 [75] | ||||
| Leupaxin | CAKb/Pyk2 [76] |
Complete References for Interacting Proteins in Table 1 of Wang & Gilmore, Biochimica et Biophysica Acta – Molecular Cell Research
5 Yang, L. et al. (2000) Interaction of the t2 transcriptional activation domain of glucocorticoid receptor with a novel steroid receptor coactivator, Hic-5, which localizes to both focal adhesions and the nuclear matrix. Mol Biol Cell 11, 2007-2018
8 Petit, M. M. et al. (2000) LPP, an actin cytoskeleton protein related to zyxin, harbors a nuclear export signal and transcriptional activation capacity. Mol Biol Cell 11, 117-129
15 Lee, J. W. et al. (1995) Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Mol Endocrinol 9, 243-254
17 Koedood Zhao, M. et al. (1999) LIM domain-containing protein Trip6 can act as a coactivator for the v-Rel transcription factor. Gene Expr 8, 207-217
18 Fujimoto, N. et al. (1999) Cloning and characterization of androgen receptor coactivator, ARA55, in human prostate. J Biol Chem 274, 8316-8321
19 Missero, C. et al. (2001) The DNA glycosylase T:G mismatch-specific thymine DNA glycosylase represses thyroid transcription factor-1-activated transcription. J Biol Chem 276, 33569-33575
22 Degenhardt, Y. Y. and Silverstein, S. (2001) Interaction of zyxin, a focal adhesion protein, with the E6 protein from human papillomavirus type 6 results in its nuclear translocation. J Virol 75, 11791-11802
31 Yi J. et al. (2002) Members of the zyxin family of LIM proteins interact with members of the p130Cas family of signal transducers. J Biol Chem 277, 9580-9589
32 Tong, X. and Howley, P. M. (1997) The bovine papillomavirus E6 oncoprotein interacts with paxillin and disrupts the actin cytoskeleton. Proc Natl Acad Sci USA 94, 4412-4417
33 Vande Pol, S. B. et al. (1998) Association of bovine papillomavirus type 1 E6 oncoprotein with the focal adhesion protein paxillin through a conserved protein interaction motif. Oncogene 16, 43-52
34 Crawford, A. W. et al. (1992) An interaction between zyxin and alpha-actinin. J Cell Biol 116, 1381-1393
35 Drees, B. E. et al. (1999) Molecular dissection of zyxin function reveals its involvement in cell motility. J Cell Biol 147, 1549-1560
36 Reinhard, M. et al. (1999) An a-actinin binding site of zyxin is essential for subcellular zyxin localization and alpha-actinin recruitment. J Biol Chem 274, 13410-13418
37 Reinhard, M. et al. (1995) Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein). Proc Natl Acad Sci USA 92, 7956-7960
38 Drees, B. et al. (2000) Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins. J Biol Chem 275, 22503-22511
39 Sadler, I. et al. (1992) Zyxin and cCRP: two interactive LIM domain proteins associated with the cytoskeleton. J Cell Biol 119, 1573-1587
40 Hirota, T. et al. (2000) Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor. J Cell Biol 149, 1073-1086
41 Hobert, O. et al. (1996) SH3 domain-dependent interaction of the proto-oncogene product Vav with the focal contact protein zyxin. Oncogene 12, 1577-1581
42 Williams, J. M. et al. (1998) Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth. Mol Microbiol 27, 171-186
43 Murthy, K. K. et al. (1999) ZRP-1, a zyxin-related protein, interacts with the second PDZ domain of the cytosolic protein tyrosine phosphatase hPTP1E. J Biol Chem 274, 20679-20687
44 Cuppen, E. et al. (2000) The zyxin-related protein TRIP6 interacts with PDZ motifs in the adaptor protein RIL and the protein tyrosine phosphatase PTP-BL. Eur J Cell Biol 79, 283-293
45 Goyal, R. K. et al. (1999) Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner. Mol Cell Biol 19, 4379-4389
46 Marie, H. et al. (2002) The amino terminus of the glial glutamate transporter GLT-1 interacts with the LIM protein Ajuba. Mol Cell Neurosci 19, 152-164
47 Schaller, M. D. et al. (1995) Focal adhesion kinase and paxillin bind to peptides mimicking b integrin cytoplasmic domains. J Cell Biol 130, 1181-1187
48 Liu, S. et al. (1999) Binding of paxillin to a4 integrins modifies integrin-dependent biological responses. Nature 402, 676-681
49 Chen, L. M. et al. (2000) Association of b1 integrin with focal adhesion kinase and paxillin in differentiating Schwann cells. J Neurosci 20, 3776-3784
50 Wine, R. N. et al. (2002) Identification of components of protein complexes using a fluorescent photo-cross-linker and mass spectrometry. Anal. Chem. 74, 1939-1945
51 Denhez, F. et al. (2002) Syndesmos, a syndecan-4 cytoplasmic domain interactor, binds to the focal adhesion adaptor proteins paxillin and hic-5. J Biol Chem 277, 12270-12274
52 Brown, M. C. and Turner, C. E. (2002) Roles for the tubulin- and PTP-PEST-binding paxillin LIM domains in cell adhesion and motility. Int. J. Biochem. Cell Biol. 34, 855-863
53 Turner, C. E. et al. (1990) Paxillin: a new vinculin-binding protein present in focal adhesions. J Cell Biol 111, 1059-1068
54 Turner, C. E. and Miller, J. T. (1994) Primary sequence of paxillin contains putative SH2 and SH3 domain binding motifs and multiple LIM domains: identification of a vinculin and pp125Fak-binding region. J Cell Sci 107, 1583-1591
55 Wood, C. K. et al. (1994) Characterization of the paxillin-binding site and the C-terminal focal adhesion targeting sequence in vinculin. J Cell Sci 107, 709-717
56 Brown, M. C. et al. (1996) Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding. J Cell Biol 135, 1109-1123
57 Nikolopoulos, S. N. and Turner, C. E. (2000) Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion. J Cell Biol 151, 1435-1448
58 Hildebrand, J. D. et al. (1995) Paxillin, a tyrosine phosphorylated focal adhesion-associated protein binds to the carboxyl terminal domain of focal adhesion kinase. Mol Biol Cell 6, 637-647
59 Salgia, R. et al. (1996) The related adhesion focal tyrosine kinase forms a complex with paxillin in hematopoietic cells. J Biol Chem 271, 31222-31226
60 Nikolopoulos, S. N. and Turner, C. E. (2001) Integrin-linked kinase (ILK) binding to paxillin LD1 motif regulates ILK localization to focal adhesions. J Biol Chem 276, 23499-23505
61 Weng, Z. et al. (1993) Detection of Src homology 3-binding proteins, including paxillin, in normal and v-Src-transformed Balb/c 3T3 cells. J Biol Chem 268, 14956-14963
62 Turner, C. E. et al. (1999) Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: a role in cytoskeletal remodeling. J Cell Biol 145, 851-863
63 West, K. A. et al. (2001) The LD4 motif of paxillin regulates cell spreading and motility through an interaction with paxillin kinase linker (PKL). J Cell Biol 154, 161-176
64 Hashimoto, S. et al. (2001) Interaction of paxillin with p21-activated Kinase (PAK). Association of paxillin alpha with the kinase-inactive and the Cdc42-activated forms of PAK3. J Biol Chem 276, 6037-6045
65 Sabe, H. et al. (1994) Analysis of the binding of the Src homology 2 domain of Csk to tyrosine-phosphorylated proteins in the suppression and mitotic activation of c-Src. Proc Natl Acad Sci USA 91, 3984-3988
66 Schaller, M. D. and Parsons, J. T. (1995) pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for Crk. Mol Cell Biol 15, 2635-2645
67 Cote, J. F. et al. (1999) Intact LIM 3 and LIM 4 domains of paxillin are required for the association to a novel polyproline region (Pro 2) of protein-tyrosine phosphatase-PEST. J Biol Chem 274, 20550-20560
68 Woods, A. J. et al. (2002) Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells. J Biol Chem 277, 6428-6437
69 Thomas, S. M. et al. (1999) Characterization of a focal adhesion protein, Hic-5, that shares extensive homology with paxillin. J Cell Sci 112, 181-190
70 Fujita, H. et al. (1998) Interaction of Hic-5, a senescence-related protein, with focal kinase. J Biol Chem 273, 26516-26521
71 Ishino, K. et al. (2000) Specific decrease in the level of Hic-5, a focal adhesion protein, during immortalization of mouse embryonic fibroblasts, and its association with focal adhesion kinase. J Cell Biochem 76, 411-419
72 Matsuya, M. et al. (1998) Cell adhesion kinase b forms a complex with a new member, Hic-5, of proteins localized at focal adhesions. J Biol Chem 273, 1003-1014
73 Ishino, M. et al. (2000) Phosphorylation of Hic-5 at tyrosine 60 by CAKb and Fyn. FEBS Lett 474, 179-183
74 Nishiya, N. et al. (1999) Hic-5, a paxillin homologue, binds to the protein-tyrosine phosphatase PEST (PTP-PEST) through its LIM 3 domain. J Biol Chem 274, 9847-9853
75 Nishiya, N., Shirai, T., Suzuki, W., Nose, K. (2002) Hic-5 Interacts with GIT1 with a different binding mode from paxillin. J Biochem (Tokyo) 132, 279-289
76 Lipsky, B. P. et al. (1998) Leupaxin is a novel LIM domain protein that forms a complex with PYK2. J Biol Chem 273, 11709-11713
77 Fernandez-Valle, C., Tang, Y., Ricard, J., Rodenas-Ruano, A., Taylor, A., Hackler, E., Biggerstaff, J., Iacovelli, J. (2002) Paxillin binds schwannomin and regulates its density- dependent localization and effect on cell morphology. Nat Genet 31, 354-362
78 Carneiro, A. M., Ingram, S. L., Beaulieu, J. M., Sweeney, A., Amara, S. G., Thomas, S. M., Caron, M. G., Torres, G. E. (2002) The multiple LIM domain-containing adaptor protein Hic-5 synaptically colocalizes and interacts with the dopamine transporter. J Neurosci 22, 7045-7054
79 Wang, Y. (2002) A molecular and cellular characterization of Trip6 (thyroid hormone receptor interacting protein 6), a LIM domain protein that moves between focal adhesion plaques and the nucleus. PhD thesis, Boston University
