|Familiarize yourself with basic flow cytometry concepts
||We ask all new users to complete the mandatory online training which includes the “Introduction to Flow Cytometry” course offered by BD Biosciences and a brief quiz. The online training is provided through the BU Blackboard, and you will be enrolled in this training once you complete the New User form.
|Attend a FCCF training session
||After you receive a passing grade on the quiz, you may attend one of our first round training sessions. There are separate training sessions for the BD LSR II SORP and the BD FACSCalibur/FACScan. Generally, each of the training sessions is provided biweekly at 9:30 AM on Wednesdays (650 Albany St, room X320). You will receive the details about the upcoming training dates in the email. This round of training is free of charge. If a new user of our Facility has significant experience on their instrument of choice, this first round training session may be waived.
|Perform your first experiment with our assistance
||After the first training session, you will be granted access to all FCCF premises and will be able to reserve the instrument of choice. If you need help with experimental design, you are welcome to request a consulting session. Once you are ready to perform your first flow cytometry experiment, contact us and book the instrument through the scheduler. Make sure you are using FCCF-approved tubes. We highly recommend use of capture compensation beads for your single stain controls. You will run the instrument while observed by a FCCF staff member. If proficiency on the instrument is observed, your training will be complete and you will receive full access to the “Cell Analysis” reservation scheduler on the instrument. However, at any time if you wish to run another experiment with assistance, feel free to book an “Assisted” session, and one of our staff members will help you with the acquisition of your data.
|Safety Training Requirements
||Make sure that you are up to date with your BSL1 and BSL2 lab safety training (included in the Research Safety Training that is mandatory for all researchers on campus).
If you are planning to use the UV laser on the BD LSR II analyzer, please complete the Laser Safety Training at the EHS website.