Our group has never used the FCCF

Create a billing account for your lab or company First, your lab/group must be registered with the Cores Equipment Scheduler. Your PI or your grant administrator needs to create a “Funding Profile” for the source of funding that you will use with the FCCF. The Cores Equipment Scheduler manages all reservations and payments.
Commercial/corporate research groups are welcome to contact us (flowcore@bu.edu) to create a service agreement and establish an account with us.

Our group has a new billing account

Adding or updating a billing account for your lab or company If you want to start using a new billing account, this procedure has to be initiated by you group since FCCF staff has no control over your group’s financial accounts. Your PI or your grant administrator needs to create a “Funding Profile” for each new source of funding before you can use it in your scheduler profile. If you try to add the source to your scheduler profile before a funding profile is created, you will receive the “account not in database” error. Also, if the account you have been using is marked as “Expired” but in fact is still valid, the same person can edit the expiration date on the existing funding profile. Please notify the Cores financial administrator by emailing ngreen2@bu.edu if you adjust the expiration date on your funding profile.

I have never used the FCCF myself

Create a personal user profile If you have never booked instruments and services in other Cores, you need to create a profile in the Cores Equipment Scheduler. During profile creation, use the account number(s) that your PI has specified when creating Funding Profile(s). You can create a profile without specifying account numbers, however, you will not be able to make reservations.

Contact the FCCF Please complete the FCCF New User Form. A FCCF staff member will contact you after your submission has been received.

I need to perform flow cytometry analysis

Familiarize yourself with basic flow cytometry concepts We ask all new users to complete the mandatory online training which includes the “Introduction to Flow Cytometry” course offered by BD Biosciences and a brief quiz. The online training is provided through the BU Blackboard, and you will be enrolled in this training once you complete the New User form.
Attend a FCCF training session After you receive a passing grade on the quiz, you may attend one of our first round training sessions. There are separate training sessions for the BD LSR II SORP, BD FACSCANTO and the BD FACSCalibur. Generally, each of the training sessions is provided biweekly at 650 Albany St, room X320. You will receive the details about the upcoming training dates in the email. This round of training is free of charge. If a new user of our Facility has significant experience on their instrument of choice, this first round training session may be waived.
Perform your first experiment with our assistance After the first training session, you will be granted access to all FCCF premises and will be able to reserve the instrument of choice. If you need help with experimental design, you are welcome to request a consulting session. Once you are ready to perform your first flow cytometry experiment, contact us and book the instrument through the scheduler. Make sure you are using FCCF-approved tubes. We highly recommend use of capture compensation beads for your single stain controls. You will run the instrument while observed by a FCCF staff member. If proficiency on the instrument is observed, your training will be complete and you will receive full access to the “Cell Analysis” reservation scheduler on the instrument. However, at any time if you wish to run another experiment with assistance, feel free to book an “Assisted” session, and one of our staff members will help you with the acquisition of your data.
Safety Training Requirements Make sure that you are up to date with your BSL1 and BSL2 lab safety training (included in the Research Safety Training that is mandatory for all researchers on campus).
If you are planning to use the UV laser on the BD LSR II analyzer, please complete the Laser Safety Training at the EHS website (scroll down for laser safety training option).

I only need to sort cells

Prepare your samples for sorting You are not required to complete our training program (above) to use our sorting services on the MoFlo Astrios or BD ARIA II SORP. However, we recommend that all our sorting users complete the “Introduction to Flow Cytometry” course offered by BD Biosciences if they are not experienced in flow cytometry. Also, please contact the FCCF and describe your sorting plans. Our sorting specialists will discuss with you which machine is optimal for your sort, the required controls that you must bring to the sort, and how much time you need to reserve for your first session. It is essential that our sorting users follow the correct biosafety practices at all times, as described here.

I need help planning my experiments

Request a consulting session We can help you to design your flow experiments (provide staining protocols, help you choose the best antibodies/other reagents to order, etc). Please email us if you want to meet and discuss your flow needs. To schedule a consulting session, please make a reservation.

I need access to the facility

If you need to obtain card access to FCCF (650 Albany St., room X320), please contact us, and we will update the security list.