PUBLICATIONS NOT IN PUB MED: 

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Arps, P.J., Marvel, C.C., Rubin, B.C., Tolan, D.R., Penhoet, E.E. and Winkler, M.E. (1985) Structural features of the hisT operon of Escherichia coli K-12.  Nucleic Acids Research  14, 5297-5315.
 
Barr, P.J., Thayer, R., Laybourne, P., Najarian, R.C., Seela, F. and Tolan, D.R. (1986) 7-Deaza-2'-deoxy-guanosine- 5'-triphosphate:  Enhanced resolution in M13 dideoxy sequencing.  BioTechniques  4, 428-432.
 
Funari, V.A., Leyfer, D. and Tolan, D.R. (2000) Expression Profiling using the Expressed Sequence Tag (EST) Database for Comparative Physiology and Metabolism. In Recent Research Developments in Comparative Biochemistry & Physiology Vol.1 (S.G. Pandalai, Ed.) Transworld Research Network, Kerala, India, pp. 13-30.
 

Barshop, B.A., Nyhan, W.L., Steenhout, P.H., Endres, W., Tolan, D.R., Clemens, R.A. (2003) Fructo-oligosaccharide tolerance in patients with hereditary fructose intolerance. A preliminary nonrandomized open challenge short-term study.  Nutrition Research  23, 1003-1011.
 
Tolan, D.R. (2008) Biochemistry Laboratory Manual.  Copley Publishing  Acton MA, 332 pp.

Tolan, D.R. (2012) Biochemistry: Laboratory Manual, 2nd Edition. XanEdu/Copley Press, Boston MA, pp. 355. [ISBN-13: 978-1-58152-889-3]

Coffee, E.M. and Tolan, D.R. (2014) Chapter 3: Gluconeogenesis, In Inborn Errors in Metabolism: From Neonatal Screening to Metabolic Pathways. Lee, B. & Scaglia, F., eds. (Oxford University Press, New York), 68-91 of 384p. [ISBN: 9780199797585].

PUBLISHED ABSTRACTS:
(underline indicates presenting author)

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1A.    Tolan, D.R., Hershey, J.W.B. and Traut, R.R. (1979) Cross-linking of eukaryotic initiation factor eIF-3 to the 40S ribosomal subunit of rabbit reticulocytes.  XIth International Congress of Biochemistry, Toronto, CANADA  8-13 July; Abstracts XIth International Congress of  Biochemistry, p. 129.

2A.    Cowgill, C., Tolan, D.R. and Traut R.R. (1981) Probing neighborhoods in eukaryotic ribosomes inferred from cross-linking with 2-iminothiolane.  XIIth International Congress of Biochemistry and III Pan-American Biochemistry Congress, Mexico City, MEXICO  22-27 June; Abstracts  International Congress of  Biochemistry, p. 269.

3A.    Tolan, D.R., Rottmann, W.H. and Penhoet, E.E. (1984)  The human aldolase B gene:   Studies toward understanding hereditary fructose intolerance. Proceedings of the 16th Miami Winter Symposium, Miami, FL  16-20 January; ICSU Short reports:  Advances in Gene Technology-Human Genetic Disorders 1, 260-261.

4A.    Tolan, D.R., Niclas, J., Lebo, R.V. and Penhoet, E.E. (1986)  Chromosomal locations of human aldolase isozymes:  Mapping a nonspherocytic hemolytic anemia disorder to chromosome 16 by spot-blot analysis. UCLA Symposium of Molecular and Cellular Biology; Amino acids in health and disease. Keystone, CO  30 May - 4 June; Journal Cellular Biochemistry  Suppl. 31, p 22.

5A.    Dazzo, C., Cox, T.M. and Tolan, D.R. (1988) Molecular evidence for heterozygosity of aldolase alleles in a fructose intolerant individual.  39th Meeting of the American Society of Human Genetics, New Orleans, LA  12-15 October; American Journal of Human Genetics  43, A5.

6A.  Podlisny, M.B., Tolan, D.R. and Selkoe, D. (1988) The monkey homolog of the beta--amyloid precursor protein of Alzheimer's Disease:  Isolation of cDNAs and detection of precursor protein. Society for Neuroscience, Toronto, CANADA  13-18 November; Abstracts  Neurosciences Meeting  14, 896.

7A.  Tolan, D.R. and Dazzo, C. (1989) Cloning and characterization of two different alleles in an individual with hereditary fructose intolerance. American Society for Biochemistry and Molecular Biology,  San Francisco, CA  29 January - 2 February; Journal of Cell Biology  107, 403a.

8A.  Podlisny, M., Gronbeck, A., Tolan, D., Oltersdorf, T. and Selkoe, D.  (1989)  Studies of the processing of beta--amyloid precursor protein in human, monkey and rodent brain and in cDNA-transfected cells.  2nd International Symposium on Familial Alzheimer's Disease,  Seattle, WA  14-17 May; Alzheimer Disease  3, 38.

9A.  Podlisny, M., Gronbeck, A., Tolan, D., Oltersdorf, T. and Selkoe, D.  (1989)  Studies of the processing of beta-amyloid precursor protein in human, monkey and rodent brain and in cDNA-transfected cells.  American Association of Neuropathologists, Dallas, TX  15-18 June; Journal of Neuropathology and Experimental Neurology  48, 353.

10A.  Podlisny, M.B., Tolan, D.R. and Selkoe, D.J. (1990) Complete homology of amyloid precursor protein in monkey and human-support for a primate model of beta--amyloidosis. May-June; Journal of Neurobiology of Aging 11, 329.

11A.  Podlisny, M.B., Ostaszewski, B., Tolan, D.R. and Selkoe, D.J. (1990) Complete homology of amyloid precursor protein in monkey and human supports a primate model of beta--amyloidosis.  American Association of Neuropathologists, San Francisco, CA  4-8 June; Journal of Neuropathology and Experimental Neurology  49, 308.

12A.  Tolan, D.R., Shaw-Lee, R. and Sullivan, D.T. (1991) Generation of three isozymes of fructose bisphosphate aldolase in Drosophila melanogaster by differential splicing.  American Society for Biochemistry and Molecular Biology,  Atlanta, GA  21-25 April; FASEB Journal   5, A1532.

13A.  Morris, A. and Tolan, D.R. (1991) Active site mutagenesis of rabbit muscle fructose 1,6 bisphosphate aldolase.  American Society for Biochemistry and Molecular Biology,  Atlanta, GA  21-25 April; FASEB Journal  5, A787.

14A.  Meighan-Mantha, R.L. and Tolan, D.R. (1991)  Cloning of discrete cDNAs of the chicken aldolase isozymes using polymerase chain reaction. American Society for Biochemistry and Molecular Biology,  Atlanta, GA  21-25 April; FASEB Journal  5, A1549.

15A.  Brooks, C.C., Tolan, D.R., Buist, N. and Tuerck, J.  (1991) Identification of a splice site mutation in the aldolase B gene from an individual with hereditary fructose intolerance.  American Society for Biochemistry and Molecular Biology,  Atlanta, GA  21-25 April; FASEB Journal  5, A440.

16A.  Brooks, C.C., Tolan, D.R., Buist, N. and Tuerck, J.  (1991) New mutations in two American patients with hereditary fructose intolerance.  8th International Congress of Human Genetics, Washington D.C.  6-11 October; American Journal of Human Genetics; Suppl.  49, 400.

17A.  Podlisny, M., Stephenson, D., Frosch, M., Tolan, D., Liederburg, I., Clemens, J. and Selkoe, D. (1991) In vivo effects of beta--amyloid protein in primates:  Microinjection study. Society for Neuroscience,  New Orleans, LA  13-18 November; Abstracts  Neurosciences Meeting  17,  1446.

18A.  Meighan-Mantha, R.L. and Tolan, D.R. (1991)  Cloning of chicken aldolase cDNAs using PCR for transcriptional analysis during myogenesis in primary cultures.  American Society for Cell Biology, Boston, MA  10-14 December; Journal of Cell Biology  115 (part 2), 454a.

19A.  Podlisny, M.B., Ostaszewski, B.L., Abrams, E., Tolan, D.R. and Selkoe, D.J. (1992) Search for mutations in the beta--amyloid precursor protein (beta-APP) by denaturing gradient-gel electrophoresis. American Association of Neuropathologists, Journal of Neuropathology and Experimental Neurology 51, 370.

20A.  Podlisny, M.B., Ostaszewski, B.L., Abrams, E., Tolan, D.R. and Selkoe, D.J.  (1992) Search for mutations in the beta--amyloid precursor protein (beta-APP) by denaturing gradient gel electrophoresis.  Third International Conference on Alzheimer's Disease & Related Disorders, Padova, ITALY  12-17 July; Neurobiology of Aging.

21A.  Podlisny, M.B., Ostaszewski, B.L., Abrams, E., Tolan, D.R. and Selkoe, D.J.  (1992) Search for mutations in the beta--amyloid precursor protein (beta-APP) by denaturing gradient gel electrophoresis. Society for Neurosciences, Anaheim, CA  25-30 October; Abstracts  Neurosciences Meeting  18, 764.

22A.  Phillips, J.L., Tolan, D.R. and Hausman, R.E. (1993) Retina-specific expression of R-cognin, a protein related to the multifunctional protein in embryonic chicken.  The American Society for Cell Biology, New Orleans, LA  11-15 December; Molecular Biology of the Cell  4, 77a.

23A.  Beernink, P.T. and Tolan, D.R. (1994) Construction of a monomeric rabbit aldolase A by site-directed mutagenesis at both subunit interfaces.  Eighth Symposium of the Protein Society   San Diego, CA  9-13 July; Protein Science, Abstracts [received Honorable Mention in Best Student Poster competition].

24A.  Tolan, D.R. and Beernink, P.T. (1995) Site-directed mutagenesis disrupts the quaternary structure of rabbit aldolase A:  Evidence for active monomers.  American Society for Biochemistry and Molecular Biology,  San Francisco, CA  21-25 May; FASEB Journal  9, A1431.

25A.  Beernink, P.T. and Tolan, D.R. (1995) Site-directed mutagenesis disrupts the quaternary structure of rabbit aldolase A:  Evidence for active monomers.  First European Symposium of the Protein Society,  Davos, SWITZERLAND  28 May - 1 June;  Protein Science, Suppl.,  4, 104.

26A.  Hausman, R.E., Phillips, J.L., Pariser, H., Ben-Shaul, Y., Tolan, D.R. and Kawamura, Y. (1995) R-cognin and cell signaling activity in retina development.  Association for Research in Vision and Ophthalmology  Ft. Lauderdale, FL  13-20 May; Investigative Ophthalmology and Vision Science 6, S776.

27A.  Phillips, J.L., Tolan, D.R. and Hausman, R.E. (1995) Antisense inhibition of retina cognin affects cell differentiation of retinal neurons in vitro.  American Society of Cell Biology, Washington D.C.  9-13 December; Molecular Biology of the Cell  6, S98A.

28A.  Wang, J., Morris, A.J., Tolan, D.R. and Pagliaro, L. (1995) The molecular nature of the F-actin binding activity of aldolase revealed with site-directed mutants.  American Society of Cell Biology, Washington D.C.  9 - 13 December; Molecular Biology of the Cell 6, S140A.

29A.  Hausman, R.E., Holdengreber, V., Phillips, J.L., Ben-Shaul, Y. and Tolan, D.R. (1996) Developmental expression of the R-cognin transcript.  Association for Research in Vision and Ophthalmology  Ft. Lauderdale, FL  21-26 April;  Investigative Ophthalmology and Vision Science 37, S149.

30A.  Wang, J., Tolan, D.R. and Pagliaro, L. (1996) Actin-binding activity localizes aldolase in living cells.  American Society of Cell Biology  Anaheim, CA  10-14 December; Molecular Biology of the Cell  7, 380a.

31A.  Berardini, T.Z. and Tolan, D.R. (1997) Aldolase gene family and isozyme evolution.  Molecular  Mechanisms of Evolution:  Structure, Function, Expression and Regulation of Genes and Proteins Keystone Symposia, Santa Fe, NM  16-21  February; Keystone Symposia on Molecular and Cellular Biology C5, 10.

32A.  Tolan, D.R., Mayr, J. and Procious, S.L. (1997) A conformational change detected by fluorescence spectroscopy is correlated to the function of fructose 1,6-bisphosphate aldolase.  Second European Symposium of the Protein Society Cambridge, UNITED KINGDOM  12-16 April; Protein Science 6, S111.

33A.  Tolan, D.R., Schuler, B., Beernink, P.T. and Jaenicke, R. (1997) Quantitative analysis of the dissociation of aldolase into active monomers using analytical ultracentrifugation.  17th International Congress of Biochemistry and Molecular Biology San Francisco, CA  24-29 August; FASEB Journal 11, A913.

34A.  Baron, C.B., Tolan, D.R. and Coburn, R.F. (1998) Ins(1,4,5)P3 binding to aldolase A mutants and interaction with Fru 1,6-P2.  FASEB -Experimental Biology '98,  San Francisco, CA  18-22 April; FASEB Journal 12, 447, A2598.

35A.  Baron, C.B., Coburn, R.F. and Tolan, D.R. (1998) Inositol (1,4,5) trisphosphate binding domain in aldolase A is not a plekstrin homology domain.  American Society of Cell Biology  San Francisco, CA  12-16 December; Molecular Biology of the Cell  8.

36A.  Malay, A.D., Procious, S.L. and Tolan, D.R. (1999) The major mutant form of aldolase B that causes hereditary fructose intolerance, A149P, has partial catalytic activity and altered quaternary structure.  The  Thirteenth Symposium of the Protein Society Boston, MA  24-28 July; Protein Science Suppl.1, 8, 534S.

37A.  Choi, H.K., Morris, A.J., Tolan, D.R. and Allen, K.N. (1999) Evidence for two alternative C6-phosphate binding sites in fructose-1,6-bis(phosphate) aldolase.  The  Thirteenth Symposium of the Protein Society Boston, MA  24-28 July; Protein Science Suppl.1, 8, 537S.

38A.  Hopkins, C.E. and Tolan, D.R. (1999) Measurement of pKa fluctuations of essential lysines in the active site of fructose 1,6-bis(phosphate) aldolase.  The  Thirteenth Symposium of the Protein Society Boston, MA  24-28 July; Protein Science Suppl.1, 8, 540S.

39A.  Funari, V.A., Leyfer, D. and Tolan, D.R. (2000) Expression profiling using a public EST database (dbEST): quantitative validation using a semi-automated probe-algorithm.  Keystone 2000:  Fulfilling the Promises of Genomic Research Taos, NM  12-14 March;

40A.  Hopkins, C.E. and Tolan, D.R. (2000) Determination of pKa of active-site lysines in rabbit muscle fructose 1,6-bisphosphate aldolase (RAMA) to elucidate their role in catalysis.  American Society for Biochemistry and Molecular Biology,  Boston, MA  4-8 June; FASEB Journal  14, A1417.

41A.  Malay, A.D., Procious, S.L. and Tolan, D.R. (2000) Structural and kinetic characterization of the aldolase B mutant A149P, the major cause of hereditary fructose intolerance.  American Society for Biochemistry and Molecular Biology,  Boston, MA  4-8 June; FASEB Journal  14, A1452.

42A.  Funari, V.A., Leyfer, D. and Tolan, D.R. (2000) A semi-automated tool for the molecular biologist: comparative in silico transcriptional profiling to identify tissues and genes involved in fructose metabolism.  American Society for Biochemistry and Molecular Biology,  Boston, MA  4-8 June; FASEB Journal  14, A1469.

43A.   Tolan, D.R., Malay, A.D. and Procious, S.L. (2001) The temperature dependence of activity and structure for the most prevalent mutant aldolase B associated with hereditary fructose intolerance:  A149P-aldolase B.  Fourth European Symposium of the Protein Society  Paris, FRANCE  18-22 April; Protein Science 10, Suppl 1, A332, p. 136.

44A.  Leyfer D., Funari V., Berwick R., Haverty P., Frith M., Tolan D.R. (2002) A Novel Algorithm for in-silico EST Expression Profiling.  3rd International Conference on Bioinformatics of Genome Regulation and Structure - BGRS 2002.  Akademgorodok, Novosibirsk, RUSSIA  14-20 July 2002; Institute of Cytology and Genetics, Novosibirsk 2, pp 186-188.

45A.  Pezza, J.A., Allen, K.N. and Tolan, D.R. (2003) Identifying unlikely determinants for isozyme specificity in fructose 1,6-bisphosphate aldolase.  Experimental Biology 2003: American Society for Biochemistry and Molecular Biology, San Diego, CA  11-15 April; FASEB Journal 17, A154.

46A.  Pezza, J.A., Allen, K.N. and Tolan, D.R. (2003) Identifying unlikely determinants for isozyme specificity in fructose 1,6-bisphosphate aldolase.  5th European Protein Symposium of the Protein Society, Florence, ITALY 29 March – 2 April; Protein Science Suppl.1, 12, A295.

47A. Pirani, A., Allen, K.N., Tolan, D.R., Craig, R., Hatch, V. and Lehman, W.H. (2004) EM and 3D Reconstruction of Dimeric Aldolase on F-Actin. Biophysical Society Meeting Long Beach CA, 12-16 February, Biophysics Journal, Abstracts Book 349Pos, B173

48A. Malay, A.D., Allen, K.N. and Tolan, D.R. (2004) The Structure and Function of the Most Prevalent Mutant Form of Aldolase B Associated with Hereditary Fructose Intolerance. 1st Pacific-Rim International Conference on Protein Science, Yokohama, JAPAN  14-18 April, New Approaches to Structure and Function of Proteins in Pacific-Rim, P17/18-072, pp 54.

49A.  Pezza, J.A., Allen, K.N. and Tolan, D.R. (2004) Conformational flexibility and distance effects determine kinetic distinctions among aldolase isozymes. American Society for Biochemistry and Molecular Biology, Boston, MA  12 - 16 June; FASEB Journal 18, C193, A78.29. [Selected as a talk]

50A.  Tolan, D.R., Funari, V.A. and Voevodski, K. (2006) Using genomic expression data for discerning tissue-specific patterns of sugar metabolism: The resourcefulness of neurons.  232nd American Chemical Society National Meeting, Division of Biological Chemistry, San Francisco, CA, 10-14 September, Chemical & Engineering News paper number 178. [Selected as a talk]

51A. Rago, F. and Tolan, D.R. (2009) Enzyme dynamics involved in substrate specificity: Evidence for differential conformational changes distant from the active site during the aldolase catalyzed reaction.The Twenty-third Symposium of the Protein Society Boston, MA 26-29 July; #104, Protein Science, 18(Suppl. 1), 86. [Selected as a talk]

52A. Stopa, J.D. and Tolan, D.R. (2009) Stabilization of a Disordered Aldolase Variant that Causes Hereditary Fructose Intolerance with Small Zwitterionic Osmolytes. The Twenty-third Symposium of the Protein Society Boston, MA 26-29 July; #157, Protein Science, 18 (Suppl. 1), 107.

53A. Ritterson Lew, C. and Tolan, D.R. (2009) Moonlighting Functions for the Glycolytic Enzyme Aldolase: Implications for Cytoskeleton Dynamics. The Twenty-third Symposium of the Protein Society Boston, MA 26-29 July; #217 Protein Science, 18 (Suppl. 1), 130-131.

54A. Coffee, E.M. and Tolan, D.R. (2009) Identification of Novel Mutations in Key Regulatory Regions of the Aldolase B Gene that cause Hereditary Fructose Intolerance. The 59th Annual Meeting of the American Society of Human Genetics. Honolulu, Hawaii, 20-24 October; #3057, The American Society of Human Genetics, Supplement, p 226.

55A. Ritterson Lew, C. and Tolan, D.R. (2009) Insights into the Warburg Effect: Aldolase Knockdown Inhibits the Growth of Ras-Transformed NIH-3T3 Cells. The 49th Annual Meeting of the American Society of Cell Biology San Diego, CA, 5-9 December, #1623, The American Society of Cell Biology, Supplement, p 119.

ABSTRACTS OF PRESENTATIONS:
(Underlined author was presenter)

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1.    Marvel, C.C., Tolan, D.R. and Nonet, M.L. (1987)  Identification of additional genes on the hisT operon.   12th International Workshop on tRNA,  Umea, SWEDEN  3-9 July.

2.    Tolan, D.R., Niclas, J., Bruce, B. and Lebo, R.V. (1987) Evolutionary relationships of the human aldolase A, B, C, and pseudo-gene chromosome locations.  XII International Meeting of the Society for Analytical Cytology,  Cambridge, UK  9-15 August.

3.    Tolan, D.R., Cross, N.P.C. and Cox, T.M. (1988) A single point mutation in human liver aldolase is responsible for hereditary fructose intolerance.  IIIICN-UCLA Symposium,  San Diego CA  17-20 March.

4.    Tolan, D.R., Cox, T.M. and Dazzo, C. (1988) Molecular evidence for heterozygosity of aldolase alleles in a fructose intolerant individual.  Abstracts XIVth International Congress of Biochemistry, Prague, CZECHOSLOVAKIA,  10-15 July.

5.  Berthiaume, L., Berardi, S., Beaudry, D., Tolan, D.R. and Sygusch, J. (1989) Probing isoenzyme catalysis and substrate specificity. UCLA Symposium, Park City, UT  20-22 January.

6.  Tamarin, R.H., Foley, M., Tolan, D.R. and Sheridan, M.  (1989) The use of DNA fingerprinting to determine paternity in voles.  6th Annual Meeting of the American Society of Mammalogists, Fairbanks, AK, 11-15 June.

7.  Berthiaume, L., Berardi, S., Beaudry, D., Tolan, D.R. and Sygusch, J.  (1989) Molecular basis of aldolase isozyme catalysis using site-directed mutagenesis.  Canadian Federation of Biological Sciences, Vancouver, CANADA  12-15 June.

8.  Berthiaume, L., Sygusch, J.  and Tolan, D.R. (1989)  Implications of the FDP aldolase carboxyl-terminus in product release.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  4-9 July.

9.  Dazzo, C. and Tolan, D.R. (1989)  Mutational heterogeneity in hereditary fructose intolerance (HFI) detected by direct sequencing using polymerase chain reaction (PCR).  Clinical Applications of Molecular Genetics, Boston, MA  9-12 July.

10.  Meighan-Mantha, R.L., Tolan, D.R. and Hausman, R.E. (1990)  Aldolase gene expression in avian myogenesis.  Northeast Regional Developmental Biology Conference.  Woods Hole, MA  20-22 April.

11.  Tolan, D.R. (1990) Screening and detection of hereditary fructose intolerance alleles in newborn blood samples using polymerase chain reaction. Vth International Congress - Inborn Errors of Metabolism, Asilomar, CA  1-5 June. [Selected as a talk]

12.  Podlisny, M.B., Tolan, D.R. and Selkoe, D.J. (1990) Complete homology of the amyloid beta--protein precursor in monkey and human:  Support for a primate model of beta--amyloidosis.  2nd International Conference on Alzheimer's Disease and Related Disorders, Toronto, CANADA  14-18 July.

13.  Podlisny, M.B., Tolan, D.R. and Selkoe, D.J. (1990) Complete homology of amyloid precursor protein in monkey and human supports a primate model of beta--amyloidosis.  5th Annual Scientific Poster Session:  Massachusetts Alzheimer's DiseaseResearchCenter, Boston, MA  9 November.

14.  Podlisny, M.B., Tolan, D.R. and Selkoe, D.J. (1991) Complete homology of the amyloid precursor protein in monkey and human:  Support for a Primate Model of beta--amyloidosis.  VIth International Symposium on Amyloidosis, Oslo, NORWAY  12-16 August.

15.  Sygusch, J., Berthiaume, L. and Tolan, D.R. (1991) Substrate discrimination in carboxyl-terminal aldolase chimers.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  1-5 July.

16.  Tolan, D.R., Shaw-Lee, R. and Sullivan, D.T. (1991) Identification of the isozymes of fructose 1,6 bisphosphate aldolase in Drosophila melanogaster generated by differential splicing.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  1-5 July.

17.  Tolan, D.R. and Morris, A.J. (1991) Site-directed mutagenesis identifies a previously undetected aspartate-33 as an essential residue in the catalytic mechanism of rabbit aldolase A.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  1-5 July.

18.  Podlisny, M.B., Stephenson, D., Frosch, M., Tolan, D.R., Liederburg, I., Clemens, J. and Selkoe, D. (1991) In vivo effects of Amyloid protein in primates:  Microinjection study.  6th Annual Scientific Poster Session:  Massachusetts Alzheimer's DiseaseResearchCenter, Boston, MA  22 November.

19.  Tolan, D.R. and Morris, A.J. (1992) Site-directed mutagenesis identifies a previously undetected aspartate-33 as a critical residue in the catalytic mechanism of rabbit aldolase A:  Role in catalysis.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  29 June - 3 July.

20.  Morris, A.J. and Tolan, D.R. (1993) Mutagenic studies of the mechanism of fructose 1,6-bisphosphate aldolase.  13th Enzyme Mechanisms Conference, Key Largo, FL  6-9 January.

21.  Tolan, D.R. and Morris, A.J. (1993) The role of lysine-146 in the catalytic mechanism of rabbit muscle fructose 1,6-bisphosphate aldolase.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  18-23 July.

22. Tolan, D.R. and Morris, A.J. (1994) A proposed mechanism of catalysis for rabbit aldolase A based upon site-directed mutations.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  17-22 July.

23.  Reichert, T.Z. and Tolan, D.R. (1994) Vertebrate evolution by tetraploidization:  aldolase as a model.  New England Molecular Evolution Conference, Harvard University, Cambridge, MA  5 November.

24.  Tolan, D.R. and Morris, A.J. (1995) Catalysis of Schiff-base formation:  role of glutamate in rabbit muscle fructose 1,6 bis(phosphate) aldolase.  14th Enzyme Mechanisms Conference, Scottsdale, AZ  4-8 January.

25.  Phillips, J.L., Ben-Shaul, Y., Holdengreber, V., Tolan, D.R. and Hausman, R.E.,  (1995) Retina cognin expression during retinogenesis in the chick embryo. Northeast Regional Developmental Biology Conference.  Woods Hole, MA  31 March - 2 April.

26.  Reichert, T.Z., Drygas-Williams, M. and Tolan, D.R. (1995) Origins of the aldolase gene family.  New England Molecular Evolution Conference, Yale University, Storrs, CT  11 November.

27.  Reichert, T.Z., Drygas-Williams, M. and Tolan, D.R. (1996)  Origins of the aldolase gene family.  8th Boston Area Graduate Student Symposium in Biology, Boston University School of Medicine, Boston, MA  16 March; Abstract No. 16.

28.  Procious, S.L. and Tolan, D.R. (1996)  Purification and characterization of recombinant human aldolase B as a fusion to glutathione-S-transferase:  An investigation into the biochemical nature of hereditary fructose intolerance. Boston Area Graduate Student Symposium in Biology, Boston University School of Medicine, Boston, MA  16 March; Abstract No. 17.

29.  Lau, J. and Tolan, D.R. (1996)  Development of a reverse dot-blot for the detection of hereditary fructose intolerance. Boston Area Graduate Student Symposium in Biology, Boston University School of Medicine, Boston, MA  16 March; Abstract No. 22.

30.  Berardini, T.Z., Foster, C., Procious, S.L., Mazurkie, A., Utheza, D., Allen, K.N. and Tolan, D.R. (1997) Formation and reactivity of the Schiff-base intermediates in fructose 1,6-bisphosphate aldolase and acetoacetate decarboxylase.  15th Enzyme Mechanisms Conference, Naples Beach, FL  4-8 January; Abstract 52.

31.  Berardini, T.Z. and Tolan, D.R. (1997) Aldolase gene family and isozyme evolution.  Boston Area Graduate Student Symposium in Biosciences  Harvard Medical School, Boston, MA  12 April.

32.  Berardini, T.Z. and Tolan, D.R. (1997) Aldolase gene family and isozyme evolution.  Science Day Boston University Graduate School, Boston University, Boston, MA  9 April.

33.  Procious S.L., Kreuder, J. and Tolan, D.R. (1997) Purification and characterization of the major mutant aldolase isozymes A and B that are responsible for the genetic disorders:  non-spherocytic hemolytic anemia (NSHA) and fructose intolerance (HFI). XVth International Congress - Inborn Errors of Metabolism, Vienna, AUSTRIA  21-25 May; Abstracts p. 181, P174.

34.  Pappas, D.J., Roy, C.C. and Tolan, D.R. (1997) Identification and characterization of a unique insertion mutation in a French-Canadian family with hereditary fructose intolerance. XVth International Congress - Inborn Errors of Metabolism, Vienna AUSTRIA  21-25 May; Abstracts, addendum, P274.

35.  Tolan D.R., Procious, S.L. and Mayer, J. (1997) A conformational change detected by fluorescence spectroscopy is correlated to the function of Fru 1,6-P2 aldolase. Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  13-18 July.

36.  Funari, V.A. and Tolan, D.R. (1997) An assessment of the EST databases for virtual cDNA cloning and expression analysis. 11th International Mouse Genome Conference. St. Petersburg, FL  12-16 October; Abstract No. 62.

37.  Choi, K.H., Mazurkie, A.S., Morris, A.J., Utheza, D., Tolan, D.R. and Allen, K.N.  (1999)  Structure of a Fructose-1,6-bis(phosphate) Aldolase liganded to its natural substrate in a cleavage defective mutant at 2.3 Å.  16th Enzyme Mechanisms Conference Napa, CA  4-8 January; Abstract 52.

38.  Coburn, R.F., Tolan, D.R., Choi, K.H. and Baron, C.B. (1999) Aldolase A-inositol 1,4,5-trisphosphate binding domains.  6th Joint American Society for Cell Biology/EMBO H. Dudley Wright on Membrane Trafficking and the Cytoskeleton.  Mario Negri del Sud, ITALY  26-30 June.

39.  Tolan, D.R., Allen, K.N., Choi, K.H. and Morris, A.J.  (1999) Structural and kinetic evidence for two alternative C6-phosphate binding sites in fructose-1,6-bis(phosphate) aldolase.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  11-16 July.

40.  Funari, V., Leyfer, D. and Tolan, D.R. (2000) A Semi-automated tool for the molecular biologist:  comparative in silico transcriptional profiling to identify tissues and genes involved in fructose metabolism.  Boston University Science Day, Boston University, Boston MA  29 March #97.  [This poster won the CAS Dean's Award for the best poster from the College of Arts and Sciences]

41.  Hopkins, C.E., Allen, K.N. and Tolan, D.R. (2000) Measurement of pKa of active-site lysines in fructose-1,6-bisphosphate aldolase. Boston University Science Day, Boston University, Boston MA  29 March #99.

42.  Unger, D.J. and Tolan, D.R. (2000) Creation of an aldolase temperature sensitive strain of Escherichia coli as a tool for understanding protein quaternary structure.  Boston University Science Day, Boston University, Boston MA  29 March #104.

43.  Malay, A. and Tolan, D.R. (2000) Structural and kinetic characterization of the major cause of hereditary fructose intolerance: aldolase B mutant A149P. Boston University Science Day, Boston University, Boston MA  29 March #105.

44.  Choi, K.H., Allen, K.N. and Tolan, D.R. (2000) The structure of fructose-1,6-bisphosphate aldolase complexed with the competitive inhibitor, fructose-6-phosphate: Why isn't it a substrate?  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  16-21 July.

45.  Tolan, D.R., Choi, K.H., Foster, C. and Allen, K.N. (2000) Presteady-state kinetics determine catalysis of ring-opening and involvement of Glu-187 in Schiff base formation in fructose 1,6-bisphosphate aldolase.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  16-21 July.

46.  Malay, A.D. and Tolan, D.R. (2001)  Structure as a function of temperature in AP-aldolase:  Implications for hereditary fructose intolerance.  Boston University Science Day, Boston University, Boston MA  27 March #50.

47.  Tolan, D.R., Choi, K.H., Shi, J., Hopkins, C.E. and Allen, K.N. (2001) Snapshots of catalysis: the structure of fructose 1,6-bisphosphate aldolase covalently bound to the substrate dihydroxyacetone phosphate.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  22-27 July.

48.  Leyfer, D., Funari, V.A., Haverty, P., Frith, M., Berwick, R. and Tolan, D.R. (2001) In-silico expression profiling using EST databases.  Biophysics and Bioinformatics Workshop Berlin 2001, Berlin, Germany  22-25 August.

49.  Pezza, J. and Tolan, D.R. (2002)  Fructose 1,6-bisphosphate aldolase contains clusters of isozyme-specific residues distal to the active site that confer isozyme specificity.  Boston University Science Day, Boston University, Boston MA  26 March #26.

50.  Malay, A.D. and Tolan, D.R. (2002)  Protein stability and disease:  characterization of a thermolabile aldolase involved in hereditary fructose intolerance.  Boston University Science Day, Boston University, Boston MA  26 March #79.

51.  Hopkins, C.E. and Tolan, D.R. (2002)  Interrogating the roles of active-site lysines in the aldolase mechanism.  Boston University Science Day, Boston University, Boston MA  26 March #80.

52.  Pezza, J.A., Berardini, T.Z., Beernink, P.T., Choi, K.H., Allen, K.N. and Tolan, D.R.  (2002) Spatial clustering of isozyme-specific residues in fructose 1,6-bisphosphate aldolase reveals potential sites of protein-protein interactions.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  16-21 July.

53.  Neufeld, E.J., Yao, D.C., Geva, A. and Tolan, D. R. (2002) Hemolytic anemia and severe rhabdomyolysis due to compound heterozygous mutations of the gene for erythrocyte/muscle isozyme of aldolase: ALDOA^{Arg303X/Cys338Tyr}.  44th Annual Meeting of the American Society of Hematology, Philadelphia, PA  6-10 December A847.

54.  Pezza, J., Berardini, T.Z., Allen, K.N. and Tolan, D.R. (2003)  Applying evolution to reveal unlikely determinants of enzyme specificity.  Boston University Science Day, Boston University, Boston MA  25 March #144.

55. Pezza, J.A., Pirani, A., Lehman, W., Tolan, D.R. and Allen, K.N.  (2003) Specificity and cellular roles of fructose-1,6-(bis)phosphate aldolase  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  13-18 July.

56. Pezza, J., Allen, K.N. and Tolan, D.R. (2004)  Protein splicing as a window to conformational flexibility in enzymes.  Boston University Science Day, Boston University, Boston MA  23 March #131.

57. Pirani, A., Allen, K., Tolan, D., Craig, R., Hatch, V. and Lehman, W. (2004) EM and 3D reconstruction of dimeric aldolase on F-actin.  Boston University Science Day, Boston University, Boston MA  23 March #105.

58. Tolan, D.R., Pezza, J.A. and Allen, K.N.  (2004) Conformational flexibility and distance effects determine kinetic distinctions among aldolase isozymes:  use of isozyme chimeras and intein-mediated protein-ligation.  Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Meriden, NH  18-23 July.

59.  Choi, K.H., Lew, V., Allen, K.N. and Tolan, D.R. (2005) Kinetic confirmation of a “catalytic dyad” involved in Schiff-base catalysis and its conservation among a superfamily of Schiff-base enzymes.  18th Enzyme Mechanisms Conference Asilomar, CA  4-9 January; Abstract 103.

60. Coffee, E., Herscovitch, M., Pezza, J.A., and Tolan, D.R. (2005) Human Diseases associated with mutations in aldolase genes.  Boston University Science Day, Boston University, Boston MA  29 March #93.

61. Stopa, J. and Tolan, D.R. (2005) Chemical Genetics Approach to HFI Treatment.  Boston University Science Day, Boston University, Boston MA  29 March #94.

62. Tolan, D.R., Allen, K.N., Choi, K.H., Lew, V., Foster, C., and Morris, A.J. (2005) Kinetic confirmation of a “catalytic dyad” involved in Schiff-base catalysis and its conservation among a superfamily of Schiff-base enzymes. Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Biddeford, ME  17-22 July; Abstract 56.
63. Tolan, D.R., Allen, K.N., and Pezza, J.A. (2006) Cooperative Interactions between Sets of ISR Patches:  Evidence for Conformational Changes in Aldolase during Catalysis. Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Biddeford, ME  18-22 July; Abstract 82.

64. Coyle, M. and Tolan, D.R. (2006) The manufacture and isolation of fructose 1-phosphate using mutant E. coli and ion-exchange chromatography. Eighth Beckman Scholar's Symposium, Newport beach CA, 27-29 July.

65.  McCorn-St. Fleur, M. and Tolan, D.R. (2006) The Genetic Characterization of Hereditary Fructose Intolerance (HFI).  Boston University UROP Undergraduate Research Symposium, Boston MA, 13 Oct, #35.

66.  Zee, T. and Tolan, D.R. (2006) A Multiplex Analysis of Genotypes for Human Inborn Errors in Fructose Metabolism. Boston University UROP Undergraduate Research Symposium, Boston MA, 13 Oct, #51.

67.  Coyle, M. and Tolan, D.R. (2006) The Manufacture and Isolation of Fructose 1-Phosphate Using Mutant E. coli Cells and Ion Exchange Chromatography. Boston University UROP Undergraduate Research Symposium, Boston MA, 13 Oct, #56.

68. Stopa, J. and Tolan, D.R. (2007) Curing Conformational Disease:  Drug Discovery using Chemical Genetics.  Boston University Science Day, Boston University, Boston MA  2 April #81.

69. Ritterson, C. and Tolan, D.R. (2007) Exploring the Moonlighting Functions of Aldolase through RNA Interference.  Boston University Science Day, Boston University, Boston MA  2 April #.

70. Coyle, M. and Tolan, D.R. (2007) Investigating substrate specificity in enzymes: mechanisms of fructose-1-phosphate cleavage by fructose-1,6-bisphosphate aldolase. Ninth Beckman Scholar’s Symposium, Newport beach CA, 26-28 July, #6.

71. Tolan, D.R. and Stopa, J. (2007) a chemical genetics approach for stabilizing enzymes derived from inborn errors in metabolism. Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Biddeford, ME 9-13 July; Abstract 55.

72. Shakourian, G. N., Liotta, L., and Tolan, D.R. (2007) Synthesis and biochemical characterization of pyrrolidine substrate analogues for fructose 1,6-(bis)phosphate. Boston University 10th Annual UROP Undergraduate Research Symposium, Boston MA, 19 Oct, #74.

73. Kampert, S., Zhang, L., and Tolan, D.R. (2007) Identification and cloning of genetic mutations causing hereditary fructose intolerance. Boston University 10th Annual UROP Undergraduate Research Symposium, Boston MA, 19 Oct, #39.

74. Garcia, M., Coffee, E., and Tolan, D.R. (2007) Identifying novel mutations that cause hereditary fructose intolerance. Boston University 10th Annual UROP Undergraduate Research Symposium, Boston MA, 19 Oct, #25.

75. Coyle, M. and Tolan, D.R. (2007) The Molecular cloning of ketohexokinase and applications towards studying the substrate specificity of aldolase. Boston University 10th Annual UROP Undergraduate Research Symposium, Boston MA, 19 Oct, #17.

76. Tolan, D.R. and Stopa, J. (2008) Osmolytes and their effect on stabilizing enzymes derived from inborn errors in metabolism. Gordon Conference; Enzymes, Coenzymes and Metabolic Pathways, Biddeford, ME 13-18 July; Abstract #85.

77. Gibbons, R., Coffee, E., and Tolan, D.R. (2008) Investigating the mutations for hereditary fructose intolerance in non-European ethnic groups. Boston University 11th Annual UROP Undergraduate Research Symposium, Boston MA, 17 Oct, #55.

78. Rago, F. and Tolan, D.R. (2008) The role of enzyme dynamics in aldolase substrate specificity. Boston University 11th Annual UROP Undergraduate Research Symposium, Boston MA, 17 Oct, #7 [Selected as a student research talk].

79. Tolan, D.R., Allen, K.N., and Rago, F. (2009) Enzyme dynamics involved in substrate specificity: Evidence for differential conformational changes distant from the active site during the aldolase catalyzed reaction. 20th Enzyme Mechanisms Conference Tucson, AZ 3-7 January; Abstract #95.

80. Oppelt, S.A. and Tolan, D.R. (2009) Neurons: sweeter than we thought! Genes for fructose metabolism in the brain. Boston University Science Day, Boston University, Boston MA 31 March #152.

81. Coffee, E.M. and Tolan, D.R. (2009) Identification of Novel Mutations in Key Regulatory Regions of the Aldolase B Gene that cause Hereditary Fructose Intolerance. Genome Science Institute Symposium, Boston University Medical Center, Boston MA 8 October; Abstract #26.

82. Stopa, J.D. and Tolan, D.R. (2009) Stabilization of Disordered Aldolase Variant that Causes HFI with Small Zwitterionic Osmolytes. Genome Science Institute Symposium, Boston University Medical Center, Boston MA 8 October; Abstract #56.

83. Doyle, E.H. and Tolan, D.R. (2009) The Inhibition of Ketohexokinase as a Treatment for Hereditary Fructose Intolerance. Boston University 12th Annual UROP Undergraduate Research Symposium, Boston MA, 16 Oct, #42. p 61.

84. Perez-Eguia, A.M., Stopa, J.D., and Tolan, D.R. (2009) Using Osmolytes to Purify Unstable Proteins Arising from Hereditary Fructose Intolerance. Boston University 12th Annual UROP Undergraduate Research Symposium, Boston MA, 16 Oct, #93, p 130.

85. Rago, F. and Tolan, D.R. (2009) Enzyme dynamics involved in substrate specificity: Evidence for differential conformational changes distant from the active site during the aldolase catalyzed reaction. Boston University 12th Annual UROP Undergraduate Research Symposium, Boston MA, 16 Oct, #95, p 134 [Awarded First Place (of >130 posters)].

86. Shastri, N. and Tolan, D.R. (2009) Characterization of the Structure and Function of Human Aldolase A Mutants. Boston University 12th Annual UROP Undergraduate Research Symposium, Boston MA, 16 Oct, #116, p 147.

87. Rago, F. and Tolan, D.R. (2010) Dictating Substrate Specificity Through Differential Conformational Dynamics. New England Beckman Scholars Winterfest, Newton, MA, January 23.

88. Ritterson Lew, C. and Tolan, D.R. (2010) Cancer and the Warburg Effect: Aldolase Knockdown Inhibits the Growth of Transformed Cells. Boston University Science Day, Boston University, Boston MA 30 March #89.

89. Connors, E., Ritterson Lew, C., and Tolan, D.R. (2010) Biochemical Identification of Aldolase Isozymes from Crude Protein and cDNA Samples. Boston University 13th Annual UROP Undergraduate Research Symposium, Boston MA, 22 Oct, #25, p 49.

90. Hardwick, L. and Tolan, D.R. (2010) Complete Amplification of Aldolase B Gene in Order to Genetically Diagnose Hereditary Fructose Intolerance. Boston University 13th Annual UROP Undergraduate Research Symposium, Boston MA, 22 Oct, p 86.

91. Raj, M., Coffee, E.M., and Tolan, D.R. (2010) The Effects of Polymorphisms in the Enhancer and an Upstream Hypersensitive Site on Transcription of the Aldolase B Gene. Boston University 13th Annual UROP Undergraduate Research Symposium, Boston MA, 22 Oct, #106, p 141.

92. Hill, K., Ritterson Lew, C., and Tolan, D.R. (2011) Elucidation of Aldolases Actin- and WASp-binding Sites via Site-Directed Mutagenesis. Boston University 14th Annual UROP Undergraduate Research Symposium, Boston MA, 21 Oct, #71, p 99.

93. Raj, M., Coffee, E.M., and Tolan, D.R. (2011) The Effects of Polymorphisms in the Enhancer and an Upstream Hypersensitive Site on Transcription of the Aldolase B Gene. Boston University 14th Annual UROP Undergraduate Research Symposium, Boston MA, 21 Oct, #134, p 167.

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