Familiarizes students with the theory and application of many biochemical techniques involved in protein purification and characterization, such as chromatography (HPLC, ion exchange, gel permeation, hydrophobic affinity), electrophoresis (followed by blotting techniques), and amino acid analysis. Students learn to think critically about methodology, design a purification scheme, scale it up, and troubleshoot an existing plan. Special problems with recombinant proteins are also covered.