He Lab
Jin He is a professor of physics at Florida International University. He received his PhD in biophysics from Arizona State University.
The He Lab studies nanoscale materials and systems, ranging from nanostructured materials to small organic molecule, biomolecules and polymers, individually or at small quantity. He probes or applies these nanoscale materials in controlled and confined nanoscale environments and/or in complex cellular and tissue environments. One goal of the research is to discover and understand new physics, chemistry or biology phenomena, and properties and functions at nanoscale. The other goal is to transfer the research discoveries into practical devices and tools, and to new applications that are relevant to promoting health and combating disease. To achieve the goal, the lab builds and develops sophisticated instruments to integrate electrical, electrochemical and optical techniques. They also utilize various top-down and bottom-up micro/nano fabrication techniques to fabricate novel devices.
Projects
Fabrication and Characterization of SERS Active Nanopipette for Intracellular Sensing (2019)
PROJECT DESCRIPTION
This project will fabricate gold nanorod (GNR) coated nanopipette to perform intracellular analysis using surface enhanced Raman spectroscopy(SERS).The specific research goals were to:
1. Synthesize gold nanorod (GNR) with proper aspect ratio to have adsorption peak around 780 nm.
2. Successful surface modification of GNR, including silica coating and chemical modification.
3. Fabrication functional GNR modified nanoipette for SERS measurement.
4. Work with the graduate student to perform intracellular biosensing using nanopiptte based SERS sensing.
LABORATORY MENTOR
Jing Guo
LEARNING GOALS
1. The synthesis of nanomaterials.
2. The fabrication of functional nano device
3. Learn low noise current measurement.
4. Learn Raman spectroscopy
5. Learn basic data analysis methods
6. Learn basic knowledge of nanosensor and cellular biology of cardiomyocyte.
In situ synthesis of Au nanoparticle film on PDMS for SERS substrate (2018)
ABSTRACT
Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical technique, which can tell us the chemical structure of examine sample, and it is widely used in biosensing. The advantage of SERS for biosensing is: chemically specific, non-destructive and high sensitivity. However, a challenge in SERS is the fabrication of uniform, reproducible and high enhancement substrate. There are e-beam lithography, nanosphere lithography methods used to solve this problem. Unlike the complicated technique mentioned above, we propose a very simple, large scale and affordable chemical synthesis method. We used gold nanoparticle film (AuNF) on Polydimethylsiloxane (PDMS) film as a substrate to enhance Raman signal. By using AuNF on PDMS, the H2O2 was detected at a concentration of 10 mM.
CONCLUSION
In summary, we developed a flexible, reproductive, and reliable SERS substrate with a high sensitivity at a low cost and can easily be replicable. The SERS substrate can be widely used in biological applications, not only to detect H2O2 but to detect other chemicals.
