Photonics Forum: Dr. Charles Lin, MGH

Intravital microscopy (IVM) is an enabling tool for visualizing cellbiology in vivo without removing cells from their native environment. Itis ideally suited to research involving animal models of human diseasesand provides deeper insights into how diseases progress and respond totherapy than is possible with human studies. In particular, by imagingmodels in which specific pathways are targeted using genetic orpharmacologic manipulations, or imaging animals engineered to expressfluorescent proteins as reporters of specific activities, IVM has madefundamental contributions to the elucidation of key cellular andmolecular pathways. However, as the field is rapidly embracing highthroughput techniques (e.g. massively parallel single cell sequencing) toprofile systems of pathways rather than individual pathways, we need torethink the role of IVM and align it with the needs of the new researchparadigm. Here, Dr. Lin will describe his attempt at integrating thespatial and temporal information provided by IVM with the high contentmolecular information provided by single cell RNA sequencing. His newoptical platform enables spatially-resolved molecular profiling underimage guidance. Preliminary results profiling leukemia cells in the bonemarrow will be presented.Dr. Charles Lin is the Principal Investigator at the MGH Center forSystems Biology and the Wellman Center for Photomedicine. Hislaboratory focuses on the development of advanced optical techniquesfor in vivo cell tracking and molecular imaging studies, concentrating oncharacterizing the bone marrow microenvironment and trackinghematopoietic cells from their origin in the bone marrow to theperipheral circulation and the central nervous system. He received hisB.S. in Chemistry from Yale University and his Ph.D. in PhysicalChemistry from University of Chicago.

When 10:00 am to 11:30 am on Thursday, March 5, 2020
Location 9th Floor Room 901 Photonics Center, 8 Saint Mary’s Street