{"id":52,"date":"2011-09-28T13:52:39","date_gmt":"2011-09-28T17:52:39","guid":{"rendered":"https:\/\/www.bu.edu\/sscores\/?page_id=52"},"modified":"2016-01-26T14:28:31","modified_gmt":"2016-01-26T19:28:31","slug":"skin","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/sscores\/cores\/skin\/","title":{"rendered":"Dermatopathology Core"},"content":{"rendered":"

The mission of the SScores Dermatopathology Core is to provide a full array of skin pathology services related to research questions in human systemic sclerosis and murine models, including routine histological stains, provision of existing and development of new immunohistochemical stains, and application of emerging technical advances, to submitted and existing biosamples in the Core.<\/em><\/h4>\n

\u00a0PI – Jag Bhawan, MD<\/h4>\n\n\n\n\n
Contact<\/strong><\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Salma Goummih<\/td>\n617.638.5569<\/td>\nsgoummih@bu.edu<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Dermatopathology Fee Schedule<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n
<\/td>\nEmbedding formalin-fixed skin<\/strong><\/td>\nSectioning paraffin block<\/strong><\/td>\nH&E Stain<\/strong><\/td>\nIHC Stain<\/strong><\/td>\nSpecial Stain<\/strong><\/td>\n<\/tr>\n
Human sample associated with MRSS\/clinical data;
\nsample remainder donated to core for future use.<\/td>\n		No cost<\/td>\nUp to 5 unstained slides
\nat no cost<\/td>\n		Up to 1 at
\nno cost<\/td>\n		Up to 1 at
\nno cost<\/td>\n		Up to 1 at no cost<\/td>\n<\/tr>\n
Human sample not associated with MRSS\/clinical data;
\nnot being donated for future use.<\/td>\n		$5\/sample<\/td>\n$2\/slide<\/td>\n$2\/slide<\/td>\n$15\/slide<\/td>\n$7.50\/slide<\/td>\n<\/tr>\n
Mouse sample<\/td>\n$5\/sample<\/td>\n$2\/slide<\/td>\n$2\/slide<\/td>\n$15\/slide<\/td>\n$7.50\/slide<\/td>\n<\/tr>\n
<\/td>\nEmbedding tissue into frozen block<\/strong><\/td>\nSectioning frozen block<\/strong><\/td>\nH&E Stain<\/strong><\/td>\nIHC Stain<\/strong><\/td>\nSpecial Stain<\/strong><\/td>\n<\/tr>\n
Human sample associated with MRSS\/clinical data;
\nsample remainder donated to core for future use.<\/td>\n		No cost<\/td>\nUp to 5 unstainedslides at no cost<\/td>\nUp to 1 at no cost<\/td>\nUp to 1 at no cost<\/td>\nUp to 1 at no cost<\/td>\n<\/tr>\n
Human sample not associated with MRSS\/clinical data;
\nnot being donated for future use.<\/td>\n		$10\/sample<\/td>\n$20\/slide<\/td>\n$5\/slide<\/td>\n$25\/20 slide<\/p>\n

(In house control\/
\nControl provided by investigator)<\/p>\n

 <\/td>\n

$18 slide<\/td>\n<\/tr>\n
Mouse sample<\/td>\n$10\/sample<\/td>\n$20\/slide<\/td>\n$5\/slide<\/td>\n$15\/slide<\/td>\n$7.50\/slide<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Click here to download the\u00a0Dermatopathology Request Form<\/a>
\nFirst-time Request Forms should be accompanied by a User – Core Interaction Agreement<\/a>
\nClick here to learn how to prepare fresh frozen tissue to fixed tissue sections.<\/a><\/p>\n

When submitting samples, biopsies should be immediately fixed in winterized<\/em> formalin.<\/strong> This can be purchased commercially, http:\/\/www.statlab.com\/product\/neutral-buffered-formalin-5-alcohol<\/a>\u00a0or made\u00a0(2700 mL of 36.5% formaldehyde, 1500 mL alcohol, 1500 mL water).<\/o:p><\/p>\n

Antibodies Currently Available for Immunohistochemical (IHC) Staining<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Antibody<\/strong><\/td>\nClone<\/strong><\/td>\nManufacturer <\/strong><\/td>\n<\/tr>\n
Smooth Muscle Actin<\/a><\/td>\n1A4<\/td>\nDako<\/td>\n<\/tr>\n
Von Willebrand Factor<\/a><\/td>\nF8\/86<\/td>\nDako<\/td>\n<\/tr>\n
CD163<\/a><\/td>\n10D6<\/td>\nNovocastra<\/td>\n<\/tr>\n
P300<\/td>\nC-20<\/td>\nSanta Cruz Biotechnology<\/td>\n<\/tr>\n
THY-1\/CD90<\/td>\nEPR3132<\/td>\nAbcam<\/td>\n<\/tr>\n
CD68<\/td>\nKP1<\/td>\nDako<\/td>\n<\/tr>\n
CD31<\/td>\nJC70A<\/td>\nDako<\/td>\n<\/tr>\n
PDGFR-B<\/td>\n28E1<\/td>\nCell Signaling<\/td>\n<\/tr>\n
P75\/NGFR<\/td>\nNGFR5<\/td>\nAbcam<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

We can also work with any other antibodies not listed to develop staining protocols.<\/em><\/p>\n

\"Dermpath2\"<\/a>
\n<\/em><\/p>\n

This core will provide uniform processing of skin samples from various investigators. We have a well established method of preparing 8 skin samples in one block to save costs as well as avoid variability of staining between samples. We have state of the art automated equipment for routine histopathology and immunopathology needs. The lab is equipped with a photomicroscope with a digital camera which can make excellent photomicrographs. Our image analysis system can evaluate various parameters in an objective manner.<\/p>\n

In addition to routine histopathology, immunostaining with any antibody can be performed. The director has tremendous experience with various antibodies including SMA, CD31, lymphocyte markers, CD34, and cathepsin k, most relevant to this field.<\/p>\n

Stain and interpret human and murine skin sections\"Picture2\"<\/a><\/h4>\n