{"id":316,"date":"2012-06-19T15:57:42","date_gmt":"2012-06-19T19:57:42","guid":{"rendered":"https:\/\/www.bu.edu\/sscores\/?page_id=316"},"modified":"2012-07-05T16:58:21","modified_gmt":"2012-07-05T20:58:21","slug":"serum-sample-collection-shipping-procedures","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/sscores\/cores\/proteomics\/serum-sample-collection-shipping-procedures\/","title":{"rendered":"Serum Sample Collection & Shipping Procedures"},"content":{"rendered":"

Principal<\/strong>
\nRBM Multi-Analyte (MAP) Technology can be applied to serum or plasma samples. To permit comparison across studies, the P30 Proteomics Core kindly requests that only serum samples be utilized.<\/p>\n

Materials<\/strong>
\n1) Serum separator tube (Options include: Red-top, Gold-top, or Grey\/red-top SST)
\n2) Transfer pipette
\n3) Cryovials (suggest using 1.0 mL or 1.8 mL)
\n4) Disposable gloves
\n5) Standard centrifuge<\/p>\n

Safety Implications<\/strong>
\nPlease observe proper precautions for biohazardous materials.<\/p>\n

Collection Procedure<\/strong>
\n1) Collect blood in appropriate serum separator tube.
\n2) Gently invert the tube 8-10 times, and let tube sit in an upright position for at least 15-30 minutes at room temperature to allow the blood to clot.
\n3) Label sample with appropriate study code.
\n4) *** Within 2 hours from time of collection***, <\/strong>spin the tubes at 1000 G using a standard room temperature centrifuge for 10-15 minutes.
\n5) Recover serum with transfer pipette.
\n6) Place ~0.5 mL of serum into each cryovial tube.
\n7) Clearly label the amount of serum in each tube.
\n8) Label each aliquot with appropriate study code and date of collection.
\nNo identifying information <\/strong>should be included on the label.
\n9) Freeze serum at -80\u00b0C until ready to ship.<\/p>\n

Both a Material Transfer Agreement and\u00a0Proteomics Core Submission Sheet<\/a> and<\/strong>
\nMaterial Transfer Agreement<\/a>\u00a0 must be recieved and approved\u00a0Proteomics Core at Northwestern prior to sending samples. Submit completed forms to Mary Carns at<\/strong>
\nm-carns@northwestern.edu<\/a>. Contact Mary by email or at (312) 503-1137 for inquiries or questions.<\/strong><\/p>\n

Shipping Procedure<\/strong>
\n1) Contact Sofia Podlusky at s-podlusky@northwestern.edu<\/a>\u00a0or (312) 503-1824 prior to shipment to ensure that the samples will be properly received.
\n2) Ship samples on dry ice using overnight shipping (ship only Monday through Thursday), and email the tracking number to Sofia Podlusky at s-podlusky@northwestern.edu.
\nIt is important that the serum does not thaw during transport.<\/strong>
\n3) A shipping log, detailing sample information for each cryovial shipped (study code, date collected, aliquot amount) should be included with the shipment and emailed to Sofia Podlusky at
\ns-podlusky@northwestern.edu<\/a>\u00a0for verification purposes.
\n4) Ship samples to:
\nAttn: Sofia Podlusky
\nRheumatology, Scleroderma Proteomics Core
\nNorthwestern University
\n745 N Fairbanks Ct
\nMcGaw Pavilion, M-230
\nChicago, IL 60611-3009<\/p>\n","protected":false},"excerpt":{"rendered":"

Principal RBM Multi-Analyte (MAP) Technology can be applied to serum or plasma samples. To permit comparison across studies, the P30 Proteomics Core kindly requests that only serum samples be utilized. Materials 1) Serum separator tube (Options include: Red-top, Gold-top, or Grey\/red-top SST) 2) Transfer pipette 3) Cryovials (suggest using 1.0 mL or 1.8 mL) 4) […]<\/p>\n","protected":false},"author":4558,"featured_media":0,"parent":56,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/sscores\/wp-json\/wp\/v2\/pages\/316"}],"collection":[{"href":"https:\/\/www.bu.edu\/sscores\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bu.edu\/sscores\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/sscores\/wp-json\/wp\/v2\/users\/4558"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/sscores\/wp-json\/wp\/v2\/comments?post=316"}],"version-history":[{"count":7,"href":"https:\/\/www.bu.edu\/sscores\/wp-json\/wp\/v2\/pages\/316\/revisions"}],"predecessor-version":[{"id":397,"href":"https:\/\/www.bu.edu\/sscores\/wp-json\/wp\/v2\/pages\/316\/revisions\/397"}],"up":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/sscores\/wp-json\/wp\/v2\/pages\/56"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/sscores\/wp-json\/wp\/v2\/media?parent=316"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}