{"id":27,"date":"2014-06-12T15:25:24","date_gmt":"2014-06-12T19:25:24","guid":{"rendered":"https:\/\/www.bu.edu\/sil\/?page_id=27"},"modified":"2020-11-09T17:54:23","modified_gmt":"2020-11-09T22:54:23","slug":"sample-size-requirements","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/sil\/sample-size-requirements\/","title":{"rendered":"Sample Size Requirements"},"content":{"rendered":"

<\/center>For those of you weighing your own samples, PLEASE\u00a0note the maximum sample sizes!<\/span><\/p>\n

Not all IRMS laboratories are the same, and mass spectrometers are tuned differently.<\/p>\n

We tune for maximum sensitivity, so we require smaller sample sizes for preloaded samples. Amounts listed where the lab will load the samples allow us to run duplicates and rerun samples if necessary. All remaining material will be returned to the submitter.<\/p>\n

Minimum Sample Sizes Needed for\u00a0Analysis<\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

Isotopes<\/span><\/strong><\/p>\n<\/td>\n

Sample Matrix<\/span><\/strong><\/center><\/td>\n
Volume\/Mass<\/span><\/strong><\/center><\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
Doubly-labeled Water (metabolic studies):<\/span><\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\n

18O<\/span><\/p><\/blockquote>\n<\/td>\n


\nbody fluid<\/span><\/center><\/td>\n

\n200 \u03bcl*<\/span><\/center><\/td>\n<\/tr>\n
\n

HD<\/span><\/p><\/blockquote>\n<\/td>\n


\nbody fluid<\/span><\/center><\/td>\n

\n10 \u03bcl*<\/span><\/center><\/td>\n<\/tr>\n
Other enriched water studies<\/span><\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\n

18O<\/span><\/p><\/blockquote>\n<\/td>\n


\nplant or soil fluid<\/span><\/center><\/td>\n

\n200 \u03bc<\/span>l*<\/span><\/center><\/td>\n<\/tr>\n
\n

HD<\/span><\/p><\/blockquote>\n<\/td>\n


\nplant or soil fluid<\/span><\/center><\/td>\n

\n200 \u03bc<\/span>l*<\/span><\/center><\/td>\n<\/tr>\n
Natural Abundance Fluid\/Water:<\/span><\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\n

18O<\/span><\/p><\/blockquote>\n<\/td>\n


\nfluid<\/span><\/center><\/td>\n

\n200 \u03bc<\/span>l<\/span><\/center><\/td>\n<\/tr>\n
\n

HD<\/span><\/p><\/blockquote>\n<\/td>\n


\nfluid<\/span><\/center><\/td>\n

\n200 \u03bc<\/span>l<\/span><\/center><\/td>\n<\/tr>\n
\n
\n

13C and 15N<\/span><\/p>\n<\/blockquote>\n<\/td>\n


\nplant tissue<\/span><\/center><\/td>\n

\n5-10 mg (C)<\/span><\/center><\/td>\n<\/tr>\n
<\/td>\n<\/td>\n

\n30-50 mg (N)<\/span><\/center><\/td>\n<\/tr>\n
<\/td>\n

\ndried animal tissue<\/span><\/center><\/td>\n

\n2-5 mg (C)<\/span><\/center><\/td>\n<\/tr>\n
<\/td>\n<\/td>\n

\n2-10 mg (N)<\/span><\/center><\/td>\n<\/tr>\n
<\/td>\n

\nsediment (marine, freshwater)<\/span><\/center><\/td>\n

\n2-10 mg(C)<\/span><\/center><\/td>\n<\/tr>\n
<\/td>\n<\/td>\n

\n50 mg (N)<\/span><\/center><\/td>\n<\/tr>\n
<\/td>\n

\nsoil (varies with %C)<\/span><\/center><\/td>\n

\n20-1000 mg<\/span><\/center><\/td>\n<\/tr>\n
15N<\/td>\n

\nwater for NH4, NO3<\/span><\/center><\/td>\n

\n500-1000 ml<\/span><\/center><\/td>\n<\/tr>\n
13C<\/td>\n

\nwater samples for DIC<\/span><\/center><\/td>\n

\n200 ml<\/span><\/center><\/td>\n<\/tr>\n
13C<\/td>\n

\nblood for bicarbonate <\/span><\/center><\/td>\n

\n1 ml<\/span><\/center><\/td>\n<\/tr>\n
S<\/td>\n

\nS dried animal tissue<\/span><\/center><\/td>\n

\n50 mg<\/span><\/center><\/td>\n<\/tr>\n
<\/td>\n

\ndried plant tissue<\/span><\/center><\/td>\n

\n100 mg<\/span><\/center><\/td>\n<\/tr>\n
<\/td>\n

\nsediment and soil <\/span><\/center><\/td>\n

\n100 mg<\/span><\/center><\/td>\n<\/tr>\n
<\/td>\n

\nwater samples<\/span><\/center><\/td>\n

\n1 liter<\/span><\/center><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

* a total of 400 \u03bc<\/span>l of fluid is needed. 18O for smaller samples is under development. For one analysis, 100 \u03bc<\/span>l is currently
\nrequired. <\/span><\/p>\n

Preloaded Sample Sizes<\/span><\/span><\/h3>\n\n\n\n\n\n\n\n\n\n\n\n\n
\n
Material<\/span><\/strong><\/div>\n<\/td>\n
\n
Amount<\/span><\/strong><\/div>\n<\/td>\n<\/tr>\n
\n
Plant material for C and N<\/span><\/div>\n<\/td>\n
\n
2 mg<\/span><\/div>\n<\/td>\n<\/tr>\n
Animal material for C and N<\/span><\/td>\n\n
1 mg<\/span><\/div>\n<\/td>\n<\/tr>\n
Soils\/sediments (will vary with %OM)**<\/span><\/td>\n\n
2-14 mg<\/span><\/div>\n<\/td>\n<\/tr>\n
DIN filters (N on filters)<\/span><\/td>\n\n
2-10 \u03bc<\/span>moles<\/span><\/div>\n<\/td>\n<\/tr>\n
POM filters<\/span><\/td>\n\n
call<\/span><\/div>\n<\/td>\n<\/tr>\n
Small organic samples<\/span><\/td>\n\n
call<\/span><\/div>\n<\/td>\n<\/tr>\n
Maximum amount of nitrogen per sample<\/span><\/td>\n\n
210 \u03bc<\/span>g<\/span><\/div>\n<\/td>\n<\/tr>\n
Maximum amount of carbon per sample<\/span><\/td>\n\n
1032 \u03bc<\/span>g<\/span><\/div>\n<\/td>\n<\/tr>\n
**Please contact lab manager to confirm soil size requirements.<\/span><\/td>\n\n
We are not responsible for lost data due to incorrectly weighed samples.<\/span><\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

For those of you weighing your own samples, PLEASE\u00a0note the maximum sample sizes! Not all IRMS laboratories are the same, and mass spectrometers are tuned differently. We tune for maximum sensitivity, so we require smaller sample sizes for preloaded samples. Amounts listed where the lab will load the samples allow us to run duplicates and […]<\/p>\n","protected":false},"author":8,"featured_media":0,"parent":0,"menu_order":6,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/sil\/wp-json\/wp\/v2\/pages\/27"}],"collection":[{"href":"https:\/\/www.bu.edu\/sil\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bu.edu\/sil\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/sil\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/sil\/wp-json\/wp\/v2\/comments?post=27"}],"version-history":[{"count":21,"href":"https:\/\/www.bu.edu\/sil\/wp-json\/wp\/v2\/pages\/27\/revisions"}],"predecessor-version":[{"id":134,"href":"https:\/\/www.bu.edu\/sil\/wp-json\/wp\/v2\/pages\/27\/revisions\/134"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/sil\/wp-json\/wp\/v2\/media?parent=27"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}