BU ROHP provides prevention, diagnosis, and treatment for work-related injuries and illnesses for the employees of Boston University in compliance with OSHA, CDC, and other regulatory requirements. The following information may be useful to researchers in assessing individual risk and avoiding potential health problems.<\/p>\n
Exposure to reproductive hazards can affect fetal development at all stages of pregnancy, with the first trimester being the most sensitive stage where all organs are developed. Lab workers are urged to review the guidance provided at the link below as soon as they begin planning for pregnancy or believe they are pregnant.<\/span><\/p>\n View Guidance<\/a><\/div>\n Researchers should notify their health care provider of the type of work they are doing should they have any unexplained illness. If a researcher develops any concern about a lab acquired illness or other infectious disease concern they should call Research Occupational Health Program 617-414-7647.<\/p>\n Please remember to consider obtaining the seasonal influenza vaccine annually to protect yourself and others.<\/p>\n Most workers who develop allergic reactions to laboratory animals will do so within the first twelve months of working with them. Infrequently, reactions only occur after working with animals for several years. Initially, the symptoms are present within minutes of the worker\u2019s exposure to the animals. Approximately half of allergic workers will have their initial symptoms subside and then recur three or four hours following the exposure.<\/div>\n<\/div>\n\n If additional respiratory protection is required, the worker should contact the Environmental Health and Safety Department, at (617) 638\u20138830 on Boston University Medical Campus (BUMC) or (617) 353\u20134094 on Charles River Campus (CRC). You must also complete an EHS ROHP OSHA Respiratory Medical Evaluation Questionnaire<\/a>. If you have any known or suspected animal allergies, please complete the Animal Allergy Screening form<\/a>. Completed forms should be submitted it to the Research Occupational Health Program (ROHP) at 72 E. Concord Street, Evans Building, 8th floor, Room 825.<\/p>\n A 2001 article by D.J. Harrison explores ways to control exposure to laboratory animal allergens.<\/a><\/div>\n<\/div>\n\n There are four increasing levels of containment that are called biosafety levels 1 through 4. Infectivity, severity of disease, transmissibility, and the nature of the work being conducted all play a role in how the CDC determines the level. An overview of each level is outlined on the CDC website.<\/a><\/p>\n Bites, scratches, and splashes of blood and body fluids from nonhuman primates pose numerous problems. Bacterial infections are the most frequent complication of a nonhuman primate bite. Cercopithecine Herpesvirus-1 or B Virus is a member of the herpes family occurring naturally in Macaque and possibly other old world monkeys. Most have no evidence of infection or others may have small blisters or ulcerations in the mouth, face, lips, genitalia, and\/or eye. Transmission to humans occurs by exposure to contaminated monkey saliva, secretions, or tissues. The most likely route is bites, scratches or splashes. The B Virus has never been cultured in blood. Injuries involving contact with nonhuman primate blood are potentially hazardous. Simian Immunodeficiency Virus (SIV) commonly persists without clinical manifestations in nonhuman primates. Type D Retroviruses (Simian retroviruses [SRV]) may infect some nonhuman primates. However, there is no current evidence of humans being infected with the type D retroviruses.<\/p>\n NHP Cage Changing<\/span><\/p>\n Goggles and surgical mask<\/p>\n Safety glasses, face shield and surgical mask<\/p>\n NHP Dental Cleaning<\/span><\/p>\n Goggles and surgical mask<\/p>\n n\/a<\/p>\n NHP Live Animal Transport or Removal to Transport Cage<\/span><\/p>\n Goggles and surgical mask<\/p>\n Safety glasses, face shield and surgical mask<\/p>\n Anesthetized NHP Animal transport<\/span><\/p>\n Goggles and surgical mask<\/p>\n Safety glasses, face shield and surgical mask<\/p>\n NHP Surgery on Anesthetized Animal using Surgical Microscope<\/span><\/p>\n Safety Glasses (prescription if needed) with Surgical Loupes, Surgical Mask for Lead Surgeon. All surgical assistants in room should wear goggles and surgical mask .<\/p>\n Lead surgeon currently wearing prescription eyeglasses with side shields and surgical loupes over the glasses with a surgical mask. Alternatively, surgical assistants can wear safety glasses, face shield, and surgical mask.<\/p>\n NHP Surgery on Anesthetized Animal using Surgical Loupes<\/span><\/p>\n Safety Glasses (prescription if needed) with Surgical Loupes, Surgical Mask for Lead Surgeon. All surgical assistants in room should wear goggles and surgical mask.<\/p>\n Lead surgeon currently wearing prescription eyeglasses with side shields and surgical loupes over the glasses with a surgical mask. Alternatively, surgical assistants can wear safety glasses, face shield, and surgical mask.<\/p>\n Tracheal Tube Insertion and Removal during the beginning and end of NHP Surgery<\/span><\/p>\n Goggles and surgical mask.<\/p>\n safety glasses (prescription if needed), face shield and surgical mask.<\/p>\n Perfusion of NHP at Termination Surgery<\/span><\/p>\n Completed in Fume Hood by Lead Surgeon: Involves slicing of heart ventricles, NHP anesthetized.<\/p>\n Completed in Fume Hood by Lead Surgeon: Currently wears prescription eyeglasses and surgical loupes and surgical mask.<\/p>\n Magnetic Resonance Imaging of NHP Anesthetized Animal<\/span><\/p>\n Anesthetized animals in sealed microisolator cage, safety glasses and surgical mask should be worn.<\/p>\n n\/a<\/div>\n<\/div>\n\n First aid for injuries related to exposure to nonhuman primates or their tissues is always handled as an emergency. <\/p><\/div><\/div> For puncture and lacerations with a contaminated instrument: Cleanse the wound immediately with a Betadine sponge scrub for 15 minutes. Rinse thoroughly. Notify the employee\u2019s supervisor and the veterinarian responsible for the animal. Report to ROHP, BUOHC, or the BMC Emergency Department after initiating treatment for the full 15 minutes.<\/p>\n For a splash of potentially contaminated fluid to the nose, mouth, or eyes: Flush the site for 15 minutes with water or saline solution. Notify the employee\u2019s supervisor and the veterinarian responsible for the animal. Report to ROHP, BUOHC, or the BMC Emergency Department immediately after flushing for the full 15 minutes.<\/p>\n NHP Cage Changing<\/span><\/p>\n Goggles and surgical mask<\/p>\n Safety glasses, face shield and surgical mask<\/p>\n NHP Dental Cleaning<\/span><\/p>\n Goggles and surgical mask<\/p>\n n\/a<\/p>\n NHP Live Animal Transport or Removal to Transport Cage<\/span><\/p>\n Goggles and surgical mask<\/p>\n Safety glasses, face shield and surgical mask<\/p>\n Anesthetized NHP Animal transport<\/span><\/p>\n Goggles and surgical mask<\/p>\n Safety glasses, face shield and surgical mask<\/p>\n NHP Surgery on Anesthetized Animal using Surgical Microscope<\/span><\/p>\n Safety Glasses (prescription if needed) with Surgical Loupes, Surgical Mask for Lead Surgeon. All surgical assistants in room should wear goggles and surgical mask .<\/p>\n Lead surgeon currently wearing prescription eyeglasses with side shields and surgical loupes over the glasses with a surgical mask. Alternatively, surgical assistants can wear safety glasses, face shield, and surgical mask.<\/p>\n NHP Surgery on Anesthetized Animal using Surgical Loupes<\/span><\/p>\n Safety Glasses (prescription if needed) with Surgical Loupes, Surgical Mask for Lead Surgeon. All surgical assistants in room should wear goggles and surgical mask.<\/p>\n Lead surgeon currently wearing prescription eyeglasses with side shields and surgical loupes over the glasses with a surgical mask. Alternatively, surgical assistants can wear safety glasses, face shield, and surgical mask.<\/p>\n Tracheal Tube Insertion and Removal during the beginning and end of NHP Surgery<\/span><\/p>\n Goggles and surgical mask.<\/p>\n Safety glasses (prescription if needed), face shield and surgical mask.<\/p>\n Perfusion of NHP at Termination Surgery<\/span><\/p>\n Completed in Fume Hood by Lead Surgeon: Involves slicing of heart ventricles, NHP anesthetized.<\/p>\n Completed in Fume Hood by Lead Surgeon: Currently wears prescription eyeglasses and surgical loupes and surgical mask.<\/p>\n Magnetic Resonance Imaging of NHP Anesthetized Animal<\/span><\/p>\n Anesthetized animals in sealed microisolator cage, safety glasses and surgical mask should be worn.<\/p>\n n\/a<\/div>\n<\/div>\n\n II. Introduction\/Background:<\/strong> A. Cercopithecine herpes virus 1 (CHV1), also known as B-virus, occurs naturally in macaque monkeys. The virus is enzootic among Old World macaques and usually causes minimal or undetectable morbidity in its natural host. Employees who have contact with these animals\u2019 tissues or secretions, may be exposed to B-virus through bites, scratches, cuts, contaminated needle-sticks and other contact of mucous membranes or broken skin with infected body fluids from macaques or with wet, unfixed tissues or primary cell culture material. In addition contaminated husbandry and research equipment can potentially spread B-virus.<\/p>\n B. Persons exposed to contact with blood, tissue or secretions of nonhuman primates are at risk for B-virus infection through:<\/p>\n III. Pre-exposure Requirements for Employees<\/strong><\/p>\n IV. Post-exposure Procedure Recommendations for Employees<\/strong><\/strong><\/p>\n Stop, Wash, Report<\/em><\/strong><\/p>\n A. Stop:<\/strong> If injured or exposed STOP<\/strong> what you are doing.<\/p>\n B. Wash:<\/strong><\/p>\n V. First Aid\/Post Exposure Prophylaxis<\/strong><\/p>\n Perform one of the following actions:<\/p>\nEliminating\/Reducing Workplace Risks<\/h2>\n
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\nInfection Control<\/h2>\n
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\nExposure to Laboratory Animals<\/h2>\n
Animal Allergy<\/h3>\n
What is an allergy?<\/h4>
What are the symptoms of allergic reactions to laboratory animals and when do they occur?<\/h4>
What laboratory animals are associated with allergic reactions?<\/h4>
What are the chances that a worker will develop an allergic reaction to laboratory animals?<\/h4>
Are there factors that are associated with an increased risk for developing an allergic reaction to laboratory animals?<\/h4>
What can be done to reduce the chance that a worker will develop an allergic reaction to laboratory animals?<\/h4>
CDC Summary of Animal Biosafety Levels (ABSL)<\/h3>\n
Working with Nonhuman Primates<\/h3>\n
Working with Nonhuman Primates<\/h4>
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\nGloves are worn to protect contamination of the skin. In the circumstances when awake nonhuman primates will be handled, thick leather gloves specially designed for protecting against bites and scratches should be worn.<\/li>\nDescription of Common Non-Human Primate Procedures at BUMC and Required Eye\/Face Personal<\/h5>\n
Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Working with Nonhuman Primate Tissue<\/h4>
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\nTissue, blood, and body fluids from nonhuman primates pose numerous problems. Cercopithecine Herpes virus-1 or B Virus is a member of the herpes family occurring naturally in macaque, rhesus, cynomolgus, and possibly other old world monkeys. It can be found in neurologic tissue, CSF, ocular, oral, or genital fluid secretions. B virus has never been cultured in blood. Due to the extreme morbidity and mortality of this infection in humans, special effort is taken to ensure prompt evaluation and first aid following potential exposure to B Virus.
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\nSimian Immunodeficiency Virus (SIV) commonly persists without clinical manifestations in nonhuman primates.
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\nType D Retroviruses (Simian retroviruses [SRV]) may infect some nonhuman primates. However, there is no current evidence on humans being infected with the type D retroviruses. Injuries involving contact with nonhuman primate blood are potentially hazardous. Each researcher working with NHP or their tissues is required to store serum for future reference.<\/p>\n
\nFirst aid should be performed before seeking medical care unless the injury is severe. See paragraphs below. Follow-up immediately with BU Research Occupational Health Program (ROHP) located at 72 E. Concord Street, Evans Building, 8th floor, room 825 and ROHP should be contacted immediately at (617) 414-7647. Hours are Monday through Friday from 8:00 am. \u2013 4:30 pm. On the Charles River Campus, contact and report to the BU Occupational Health Center (BUOHC) located at 930 Commonwealth Ave., West, Pleasant Street entrance, Tel: (617) 353-6630. On weekends, evenings, and holidays, report to the BMC Emergency Department, Harrison Avenue Campus, located at 840 Harrison Avenue, Menino Pavilion.<\/p>\nDescription of Common Non-Human Primate Procedures at BUMC and Required Eye\/Face Personal<\/h5>\n
Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
Alternative, if available:<\/h6>\n
Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Eye\/Face\/Mucous Membrane Protections:<\/h6>\n
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Post Exposure Procedures Following NHP Macaque Injuries (Standard Operating Procedure)<\/h4>
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