Sony SH800 Cell Sorter
We have a Sony SH800 cell sorter that can accept 1.5mL microfuge tubes, 5mL FACS tubes, 15mL centrifuge tube samples and sort cells into 5mL FACS tubes, 15mL tubes centrifuge tubes, 96 and 384 well plates. The system bills at the rate of $90/hour (BU/BMC), $120/hour (external academic) and $160/hour (external commercial). Currently, there is only staff operation. Sorting is not performed on demand and must be scheduled in advance. Email (blute at bu dot edu) or call 617 353-2638
Specs – Spreadsheet4
| Optics |
Excitation lasers |
488 nm, 405 nm, 638 nm and 561 nm laser |
| Output power |
30 mW (max.) optical fiber output |
| Beam alignment |
Collinear optical system |
| Detection Parameters |
6 fluorescent + 2 scatter |
| Analog-to-digital converters (ADC) |
8-channel 20-bit, 110MHz |
| Pulse measurement |
Height, Area, Width |
| Fluidics |
Sample tube |
Single, auto-loading tube |
| Tube types |
1.5 ml, 5 ml and 15 ml tubes |
| Sort Devices |
2-way tube, multiwell plates, |
| Temperature control |
5°C, 37°C (Electric Cooling Method) |
| Agitation unit |
Eccentric rotation |
| Magnetic drive |
300 rpm speed |
| Sorting chip size |
70 μm, 100 μm and 130 μm |
| Sort Performance |
Event rate |
100,000 eps |
| Sorting Speed |
Using the 70 μm sorting chip at 50 kHz an average threshold of 12,000 events per second can be achieved with >95% purity and >80% yield. The threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics. |
| Scatter resolution |
0.5μm |
| Fluorescence resolution |
< 2.5% Half-peak coefficient of variation (HPCV) |
| Fluorescence sensitivity |
FITC 120 MESF, PE 110 MESF |
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