{"id":2315,"date":"2023-06-21T15:02:00","date_gmt":"2023-06-21T19:02:00","guid":{"rendered":"https:\/\/www.bu.edu\/photonics-ret\/?page_id=2315"},"modified":"2023-06-21T17:37:48","modified_gmt":"2023-06-21T21:37:48","slug":"thomas-bifano-2023","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/photonics-ret\/thomas-bifano-2023\/","title":{"rendered":"High Throughput Screening of Cardiomyopathy in 3D Engineered Tissues"},"content":{"rendered":"

Project Description<\/span><\/strong><\/h4>\n

The project would involve using our 96 well-plate platform with microtugs to assess tissues from cells produced by the Adam Helms lab. We will compare results measured on our high throughput platform with those measured with Helms\u2019 2D micropatterning muscle bundle system (<\/span>https:\/\/www.nature.com\/articles\/s41467-021-26496-1<\/a>).<\/span><\/p>\n

The Helms group will thaw and transduce the iPSC-CMs at UM, re-freeze, then ship, so they’d be directly ready for making tissues<\/span><\/p>\n

Mentors<\/strong><\/span><\/h4>\n

Thomas Bifano & Francisco Sanchez<\/p>\n

Research Goals<\/h4>
There would be 2 main questions. The central question for the cardiomyopathy variants is whether we can detect differences in contraction and relaxation velocity in 3D structures measured using our 96 well platform. Robust functional assays capable of differentiating these effects would be useful. A large number of replicates will give us the ability to assess the variability well. The question is whether contractile effects of these variants are different across the \u00b5Tug platforms vs the Helms 2D micropatterned tissues. There could be advantages of the greater number of cells per tissue in the \u00b5Tugs as well as effects of being 3D tissues.<\/div>\n<\/div>\n\n

Learning Goals<\/h4>
\u2022 Learn to used 96 well platform
\n\u2022 Establish pipeline of material transfer between Helms lab and Bifano lab
\n\u2022 Design experiments to answer primary and secondary questions.<\/div>\n<\/div>\n\n

Project Timeline<\/strong><\/span><\/h4>\n

Week 1: Orientation (w\/ materials provided by Francisco Sanchez)<\/span>
\nWeek 2: Material transfer and tissue seeding at BU (w\/ Josh Lee)<\/span>
\nWeek 3: Preliminary testing<\/span>
\nWeek 4: Data analysis, additional testing<\/span>
\nWeek 5: Interpretation and presentation<\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Project Description The project would involve using our 96 well-plate platform with microtugs to assess tissues from cells produced by the Adam Helms lab. We will compare results measured on our high throughput platform with those measured with Helms\u2019 2D micropatterning muscle bundle system (https:\/\/www.nature.com\/articles\/s41467-021-26496-1). The Helms group will thaw and transduce the iPSC-CMs at […]<\/p>\n","protected":false},"author":19768,"featured_media":0,"parent":0,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/photonics-ret\/wp-json\/wp\/v2\/pages\/2315"}],"collection":[{"href":"https:\/\/www.bu.edu\/photonics-ret\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bu.edu\/photonics-ret\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/photonics-ret\/wp-json\/wp\/v2\/users\/19768"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/photonics-ret\/wp-json\/wp\/v2\/comments?post=2315"}],"version-history":[{"count":5,"href":"https:\/\/www.bu.edu\/photonics-ret\/wp-json\/wp\/v2\/pages\/2315\/revisions"}],"predecessor-version":[{"id":2351,"href":"https:\/\/www.bu.edu\/photonics-ret\/wp-json\/wp\/v2\/pages\/2315\/revisions\/2351"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/photonics-ret\/wp-json\/wp\/v2\/media?parent=2315"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}