{"id":199,"date":"2011-02-08T09:56:54","date_gmt":"2011-02-08T14:56:54","guid":{"rendered":"https:\/\/www.bu.edu\/nf-kb\/reagents\/"},"modified":"2014-03-26T09:16:03","modified_gmt":"2014-03-26T13:16:03","slug":"reagents","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/nf-kb\/other-resources\/reagents\/","title":{"rendered":"Reagents"},"content":{"rendered":"

NF-kB Reagents<\/strong><\/p>\n

This page provides information about reagents that may be valuable to researchers studying the NF-kB signaling pathway.\u00a0\u00a0This information is based on our experience (or that of others), and we do not swear to its accuracy (especially since different lots of antisera may vary).\u00a0 We welcome comments that will help us keep the information accurate. \u2028\u00a0 \u2028ANTIBODIES <\/strong>(see below for abbreviations)<\/p>\n

IKKgamma\/NEMO:\u00a0 Cell Signaling Technology<\/strong><\/a> (#2685; Hu)<\/p>\n

IKKbeta: Cell Signaling Technology<\/strong><\/a> (#2684, Hu); Upstate<\/strong><\/a> (#05535; Hu)<\/p>\n

IKKalpha:\u00a0Imgenex<\/strong><\/a> (IMG-5477; Hu, Ra, Mo)<\/p>\n

Anti-RelA\/p65: Santa Cruz<\/strong><\/a> (sc-372x; Hu\/Mo; SS)<\/p>\n

Anti-RelB: Santa Cruz<\/strong><\/a> (C-19; Hu; WB)<\/p>\n

Anti-p50\/p105: Santa Cruz<\/strong><\/a> (H-119; Hu; WB) (sc-7187x; Hu; SS) (sc-114x; Mo; SS)<\/p>\n

Anti-p52\/p100: Santa Cruz<\/strong><\/a> (K27; Hu; WB) (sc-298x; SS)<\/p>\n

Anti-IkBalpha:\u00a0 Santa Cruz<\/strong><\/a> (C15, Hu; WB)<\/p>\n

Anti-phospho-IkBalpha:\u00a0 Cell Signaling Technology<\/strong><\/a> (#9241; Hu, Mo; WB)<\/p>\n

Anti-IkBbeta: Santa Cruz<\/strong><\/a> (sc-946; Hu; WB)<\/p>\n

Anti-IkBe: Santa Cruz<\/strong><\/a> (M-121; Hu; WB)<\/p>\n

REPORTER GENE PLASMIDS<\/strong><\/p>\n

3xkB-Luc:\u00a0 3 kB sites from the MHC enhancer, upstream of luciferase gene (Starczynowski et al, 2005<\/strong><\/a>)<\/p>\n

HIV-CAT:\u00a0 the HIV-1 LTR enhancer with 2 kB sites upstream of the CAT gene (Richardson et al, 1990<\/strong><\/a>)<\/p>\n

GAL4-luc:\u00a0 GAL4 DNA-binding sites upstream of the luciferase gene (Starczynowski et al, 2005<\/strong><\/a>)<\/p>\n

cDNA EXPRESSION <\/strong>PLASMIDS<\/strong><\/p>\n

pcDNA3-based vectors are available upon request from us for the expression of the following full-length cDNAs:\u00a0 human, mouse, chicken c-rel; or v-rel; FLAG-human IKKbeta; FLAG-human IKKalpha; FLAG-human IKKgamma (NEMO).<\/p>\n

pSG424-based vectors for the expression of GAL4 fusion proteins are availabe from us for the expression of fusion proteins to:\u00a0 the transactivation domains of human, mouse, and chicken c-Rel; human p65; v-Rel; chicken and mouse RelB.<\/p>\n

Abbreviations:<\/p>\n

Hu, Human; Mo, Mouse<\/p>\n

WB, Western blot; SS, supershift<\/p>\n","protected":false},"excerpt":{"rendered":"

NF-kB Reagents This page provides information about reagents that may be valuable to researchers studying the NF-kB signaling pathway.\u00a0\u00a0This information is based on our experience (or that of others), and we do not swear to its accuracy (especially since different lots of antisera may vary).\u00a0 We welcome comments that will help us keep the information […]<\/p>\n","protected":false},"author":4258,"featured_media":0,"parent":331,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/nf-kb\/wp-json\/wp\/v2\/pages\/199"}],"collection":[{"href":"https:\/\/www.bu.edu\/nf-kb\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bu.edu\/nf-kb\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/nf-kb\/wp-json\/wp\/v2\/users\/4258"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/nf-kb\/wp-json\/wp\/v2\/comments?post=199"}],"version-history":[{"count":4,"href":"https:\/\/www.bu.edu\/nf-kb\/wp-json\/wp\/v2\/pages\/199\/revisions"}],"predecessor-version":[{"id":856,"href":"https:\/\/www.bu.edu\/nf-kb\/wp-json\/wp\/v2\/pages\/199\/revisions\/856"}],"up":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/nf-kb\/wp-json\/wp\/v2\/pages\/331"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/nf-kb\/wp-json\/wp\/v2\/media?parent=199"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}