{"id":2211,"date":"2019-10-09T10:10:28","date_gmt":"2019-10-09T14:10:28","guid":{"rendered":"https:\/\/www.bu.edu\/neurophotonics\/?page_id=2211"},"modified":"2025-06-23T11:01:37","modified_gmt":"2025-06-23T15:01:37","slug":"instruments","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/neurophotonics\/core-facility\/instruments\/","title":{"rendered":"Instruments"},"content":{"rendered":"<p>The core facility is home to a variety of imaging instruments ranging from advanced laser scanning microscopes to ultrasound imaging systems. Though our central focus is supporting and using these instruments in neuroscience research, these techniques can be applied towards other aspects of biomedical imaging. On this page, you will find technical information about the various imaging systems that is available for core users.<\/p>\n<hr \/>\n<div class=\"bu_collapsible_container \" aria-live=\"polite\" data-customize-animation=\"false\"><h3 class=\"bu_collapsible\" aria-expanded=\"false\"tabindex=\"0\" role=\"button\">fNIRS<\/h3><div class=\"bu_collapsible_section\" style=\"display: none;\"><\/p>\n<h5><img loading=\"lazy\" src=\"\/neurophotonics\/files\/2020\/05\/fNIRS-636x343.png\" alt=\"\" width=\"636\" height=\"343\" class=\"alignnone size-medium wp-image-3182\" srcset=\"https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/fNIRS-636x343.png 636w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/fNIRS-768x415.png 768w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/fNIRS-1024x553.png 1024w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/fNIRS.png 1391w\" sizes=\"(max-width: 636px) 100vw, 636px\" \/><\/h5>\n<p><a href=\"https:\/\/www.bu.edu\/neurophotonics\/research\/fnirs\/\">Functional Near Infrared Spectroscopy (fNIRS)<\/a> is a wearable brain monitoring system that uses near-infrared optical light to measure changes in the cortical hemodynamic response.<\/p>\n<ul><\/ul>\n<p><b><span lang=\"EN\">Systems and parts<\/span><\/b><\/p>\n<ul>\n<li><b><span lang=\"EN\">fNIRS System I:<\/span><\/b><span lang=\"EN\"> CW-NIRS System utilizing laser diodes at 690 and 830nm (TechEn Inc. MA, USA). The TechEn system is a multichannel continuous wave optical imager consisted of 32 frequency-encoded lasers (half of them at 690 and the other half at 830 nm) and 32 avalanche photo-diode detectors.<\/span><\/li>\n<li><b><span lang=\"EN\">fNIRS System II: <\/span><\/b><span lang=\"EN\">NinjaNIRS is a BU home-built modular and fully scalable wearable NIRS device utilizing optodes with of one dual wavelength LED (730\/850 nm) and one photodiode<\/span><\/li>\n<li><b><span lang=\"EN\">fNIRS Head Caps:<\/span><\/b><span lang=\"EN\"> 3D printing of NinjaCaps<\/span><\/li>\n<li><b><span lang=\"EN\">Fibers:<\/span><\/b><span lang=\"EN\"> Source\/detector optical fibers for fNIRS System I<\/span><\/li>\n<\/ul>\n<p><b><span lang=\"EN\">fNIRS Assistance<\/span><\/b><\/p>\n<ul>\n<li><b><span lang=\"EN\">fNIRS Data Collection:<\/span><\/b><span lang=\"EN\"><a href=\"https:\/\/openfnirs.org\/training\/\"> Training<\/a> and supervision on fNIRS data collection with both fNIRS System I and II.<\/span><\/li>\n<li><b><span lang=\"EN\">fNIRS Data Analysis:<\/span><\/b><span lang=\"EN\"><a href=\"https:\/\/openfnirs.org\/training\/\"> Training<\/a> and supervision on fNIRS data analysis using <a href=\"https:\/\/github.com\/BUNPC\/Homer3\/wiki\">HOMER3<\/a>\u00a0and <a href=\"https:\/\/github.com\/BUNPC\/AtlasViewer\/wiki\">AtlasViewer<\/a>.<\/span><\/li>\n<\/ul>\n<p><strong>Questions &#8211;\u00a0<\/strong>Please fill out <a href=\"https:\/\/forms.gle\/mYN4oUKTueCq8erp7\">this form<\/a> with any questions you may have.<br \/>\n<\/div>\n<\/div>\n\n<p>&nbsp;<\/p>\n<hr \/>\n<div class=\"bu_collapsible_container \" aria-live=\"polite\" data-customize-animation=\"false\"><h3 class=\"bu_collapsible\" aria-expanded=\"false\"tabindex=\"0\" role=\"button\">fUS\/PAT<\/h3><div class=\"bu_collapsible_section\" style=\"display: none;\"><br \/>\n<img loading=\"lazy\" src=\"\/neurophotonics\/files\/2019\/04\/ULM-vULM-636x243.jpg\" alt=\"\" width=\"529\" height=\"202\" class=\"alignnone wp-image-1970\" srcset=\"https:\/\/www.bu.edu\/neurophotonics\/files\/2019\/04\/ULM-vULM-636x243.jpg 636w, https:\/\/www.bu.edu\/neurophotonics\/files\/2019\/04\/ULM-vULM-768x294.jpg 768w, https:\/\/www.bu.edu\/neurophotonics\/files\/2019\/04\/ULM-vULM-1024x391.jpg 1024w\" sizes=\"(max-width: 529px) 100vw, 529px\" \/><\/p>\n<p><b><span lang=\"EN\">Verasonics<\/span><\/b><\/p>\n<ul>\n<li><span class=\"Apple-converted-space\">We have the 256 channel Vantage functional ultrasound system from <a href=\"http:\/\/verasonics.com\/\">Verasonics<\/a>. You can see some of the things we are doing with it <a href=\"https:\/\/www.bu.edu\/neurophotonics\/research-themes\/fus\/\">here<\/a>.<\/span><\/li>\n<li><span class=\"Apple-converted-space\">\u00a0fUS <a href=\"https:\/\/www.bu.edu\/neurophotonics\/files\/__restricted\/entire-bu-community\/Verasonics_userguide.pdf\">User Guide<\/a><\/span><\/li>\n<\/ul>\n<p><b><span lang=\"EN\">fUS\/PAT Assistance<\/span><\/b><\/p>\n<ul>\n<li>Training and experimental assistance is available through the core facility.<\/li>\n<\/ul>\n<p><strong>Questions &#8211;\u00a0<\/strong>Please fill out <a href=\"https:\/\/forms.gle\/mYN4oUKTueCq8erp7\">this form<\/a> with any questions you may have.<br \/>\n<\/div>\n<\/div>\n\n<p>&nbsp;<\/p>\n<hr \/>\n<div class=\"bu_collapsible_container \" aria-live=\"polite\" data-customize-animation=\"false\"><h3 class=\"bu_collapsible\" aria-expanded=\"false\"tabindex=\"0\" role=\"button\">2-Photon Microscopy<\/h3><div class=\"bu_collapsible_section\" style=\"display: none;\"><\/p>\n<p><div id='gallery-1' class='gallery galleryid-2211 gallery-columns-4 gallery-size-responsive_gallery'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='\/neurophotonics\/files\/2020\/05\/SingleImage-03292018-1118-095-Window1-Ch1-Ch2-8bit-Reference.jpg'><img width=\"512\" height=\"512\" src=\"\/neurophotonics\/files\/2020\/05\/SingleImage-03292018-1118-095-Window1-Ch1-Ch2-8bit-Reference.jpg\" class=\"attachment-responsive_gallery size-responsive_gallery\" alt=\"\" loading=\"lazy\" aria-describedby=\"gallery-1-3252\" srcset=\"https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/SingleImage-03292018-1118-095-Window1-Ch1-Ch2-8bit-Reference.jpg 512w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/SingleImage-03292018-1118-095-Window1-Ch1-Ch2-8bit-Reference-150x150.jpg 150w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/SingleImage-03292018-1118-095-Window1-Ch1-Ch2-8bit-Reference-511x512.jpg 511w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/SingleImage-03292018-1118-095-Window1-Ch1-Ch2-8bit-Reference-100x100.jpg 100w\" sizes=\"(max-width: 512px) 100vw, 512px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-3252'>\n\t\t\t\tReference image for calcium imaging \n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='\/neurophotonics\/files\/2020\/05\/MAX_ZSeries-09282018-TPM_Eliza-001.jpg'><img width=\"550\" height=\"550\" src=\"\/neurophotonics\/files\/2020\/05\/MAX_ZSeries-09282018-TPM_Eliza-001.jpg\" class=\"attachment-responsive_gallery size-responsive_gallery\" alt=\"\" loading=\"lazy\" aria-describedby=\"gallery-1-3249\" srcset=\"https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/MAX_ZSeries-09282018-TPM_Eliza-001.jpg 1024w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/MAX_ZSeries-09282018-TPM_Eliza-001-150x150.jpg 150w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/MAX_ZSeries-09282018-TPM_Eliza-001-636x636.jpg 636w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/MAX_ZSeries-09282018-TPM_Eliza-001-768x768.jpg 768w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/MAX_ZSeries-09282018-TPM_Eliza-001-700x700.jpg 700w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/MAX_ZSeries-09282018-TPM_Eliza-001-100x100.jpg 100w\" sizes=\"(max-width: 550px) 100vw, 550px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-3249'>\n\t\t\t\tin-vivo 2P Angiogram (FITC-Dextran)\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='\/neurophotonics\/files\/2020\/05\/FITCSR101.png'><img width=\"467\" height=\"466\" src=\"\/neurophotonics\/files\/2020\/05\/FITCSR101.png\" class=\"attachment-responsive_gallery size-responsive_gallery\" alt=\"\" loading=\"lazy\" aria-describedby=\"gallery-1-3260\" srcset=\"https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/FITCSR101.png 467w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/FITCSR101-150x150.png 150w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/FITCSR101-100x100.png 100w\" sizes=\"(max-width: 467px) 100vw, 467px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-3260'>\n\t\t\t\tMulti-channel Fluorescent Images of FITC and SR101\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='\/neurophotonics\/files\/2020\/05\/IMG_0034-e1589990745866.jpg'><img width=\"454\" height=\"550\" src=\"\/neurophotonics\/files\/2020\/05\/IMG_0034-e1589990745866.jpg\" class=\"attachment-responsive_gallery size-responsive_gallery\" alt=\"\" loading=\"lazy\" aria-describedby=\"gallery-1-3251\" srcset=\"https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/IMG_0034-e1589990745866.jpg 2993w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/IMG_0034-e1589990745866-525x636.jpg 525w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/IMG_0034-e1589990745866-768x931.jpg 768w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/IMG_0034-e1589990745866-845x1024.jpg 845w\" sizes=\"(max-width: 454px) 100vw, 454px\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-1-3251'>\n\t\t\t\t2P device\n\t\t\t\t<\/figcaption><\/figure>\n\t\t<\/div>\n<\/p>\n<p><b><span lang=\"EN\">Bruker System<\/span><\/b><\/p>\n<ul>\n<li>We have a Bruker <a href=\"https:\/\/www.bruker.com\/products\/fluorescence-microscopes\/ultima-multiphoton-microscopy\/ultima-investigator\/overview.html\">Ultima Investigator<\/a> Two-Photon Microscope with fluorescence and phosphorescence lifetime capabilities.<\/li>\n<li>Bruker 2-photon <a href=\"https:\/\/www.bu.edu\/neurophotonics\/files\/2019\/02\/Bruker-Two-Photon-Microscopy-Guide.pdf\">user guide<\/a><\/li>\n<\/ul>\n<p><strong>Questions &#8211;\u00a0<\/strong>Please fill out <a href=\"https:\/\/forms.gle\/mYN4oUKTueCq8erp7\">this form<\/a> with any questions you may have.<br \/>\n<\/div>\n<\/div>\n\n<hr \/>\n<div class=\"bu_collapsible_container \" aria-live=\"polite\" data-customize-animation=\"false\"><h3 class=\"bu_collapsible\" aria-expanded=\"false\"tabindex=\"0\" role=\"button\">Speckle\/Spectral\/SFDI Imaging<\/h3><div class=\"bu_collapsible_section\" style=\"display: none;\"><\/p>\n<p><div id='gallery-2' class='gallery galleryid-2211 gallery-columns-3 gallery-size-responsive_gallery'><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon portrait'>\n\t\t\t\t<a href='\/neurophotonics\/files\/2020\/05\/Imag2.jpg'><img width=\"275\" height=\"538\" src=\"\/neurophotonics\/files\/2020\/05\/Imag2.jpg\" class=\"attachment-responsive_gallery size-responsive_gallery\" alt=\"\" loading=\"lazy\" aria-describedby=\"gallery-2-3272\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-2-3272'>\n\t\t\t\tResting state functional connectivity maps\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='\/neurophotonics\/files\/2020\/05\/Imag3.jpg'><img width=\"438\" height=\"338\" src=\"\/neurophotonics\/files\/2020\/05\/Imag3.jpg\" class=\"attachment-responsive_gallery size-responsive_gallery\" alt=\"\" loading=\"lazy\" aria-describedby=\"gallery-2-3273\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-2-3273'>\n\t\t\t\tFunctional activation during forepaw stimulation\n\t\t\t\t<\/figcaption><\/figure><figure class='gallery-item'>\n\t\t\t<div class='gallery-icon landscape'>\n\t\t\t\t<a href='\/neurophotonics\/files\/2020\/05\/Imag4.jpg'><img width=\"550\" height=\"399\" src=\"\/neurophotonics\/files\/2020\/05\/Imag4.jpg\" class=\"attachment-responsive_gallery size-responsive_gallery\" alt=\"\" loading=\"lazy\" aria-describedby=\"gallery-2-3274\" \/><\/a>\n\t\t\t<\/div>\n\t\t\t\t<figcaption class='wp-caption-text gallery-caption' id='gallery-2-3274'>\n\t\t\t\tChange in scattering coefficient after stroke\n\t\t\t\t<\/figcaption><\/figure>\n\t\t<\/div>\n<\/p>\n<p><span>This is a home built wide-field microscope that can simultaneously obtain relative changes to cerebral blood flow using laser speckle contrast imaging and changes to oxy- and deoxy-hemoglobin using intrinsic optical signal imaging. Details of the combined speckle and spectral imaging system is described <\/span><u><a href=\"https:\/\/www.bu.edu\/neurophotonics\/research-themes\/mslsi\/\">here<\/a><\/u><span>. This system has also been integrated with a spatial frequency domain imaging setup based on the designs provided by Professor Roblyer\u2019s group as described <\/span><u><a href=\"http:\/\/opensfdi.org\/\">here<\/a><\/u><span>.<\/span><\/p>\n<p><strong>Questions &#8211;\u00a0<\/strong>Please fill out<a href=\"https:\/\/forms.gle\/mYN4oUKTueCq8erp7\"> this form<\/a> with any questions you may have.<br \/>\n<\/div>\n<\/div>\n\n<hr \/>\n<div class=\"bu_collapsible_container \" aria-live=\"polite\" data-customize-animation=\"false\"><h3 class=\"bu_collapsible\" aria-expanded=\"false\"tabindex=\"0\" role=\"button\">Optical Coherence Tomography<\/h3><div class=\"bu_collapsible_section\" style=\"display: none;\"><br \/>\n<img loading=\"lazy\" src=\"\/neurophotonics\/files\/2020\/05\/IMG_1693-636x477.jpg\" alt=\"\" width=\"354\" height=\"266\" class=\"alignnone wp-image-3156\" srcset=\"https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/IMG_1693-636x477.jpg 636w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/IMG_1693-768x576.jpg 768w, https:\/\/www.bu.edu\/neurophotonics\/files\/2020\/05\/IMG_1693-1024x768.jpg 1024w\" sizes=\"(max-width: 354px) 100vw, 354px\" \/><\/p>\n<p>We have a <a href=\"https:\/\/www.thorlabs.com\/newgrouppage9.cfm?objectgroup_id=12569\">Thorlabs Telesto OCT<\/a> system with an integrated laser speckle (LS) camera.<\/p>\n<p>More details about OCT can be found on our <a href=\"https:\/\/www.bu.edu\/neurophotonics\/research\/oct\/\">OCT research page<\/a>.<\/p>\n<p><strong>Questions &#8211;\u00a0<\/strong>Please fill out <a href=\"https:\/\/docs.google.com\/forms\/d\/e\/1FAIpQLSfFvQVTEG_6_mmXw_swXRZWNAqjQhfbZlM4S8uTTQVZolVwTw\/viewform\">this form<\/a> with any questions you may have.<br \/>\n<\/div>\n<\/div>\n\n<hr \/>\n<div class=\"bu_collapsible_container \" aria-live=\"polite\" data-customize-animation=\"false\"><h3 class=\"bu_collapsible\" aria-expanded=\"false\"tabindex=\"0\" role=\"button\">Microscope Imaging Assistance<\/h3><div class=\"bu_collapsible_section\" style=\"display: none;\"><br \/>\nInformation for assistance with the use of specific instruments can be found on the <a href=\"https:\/\/www.bu.edu\/neurophotonics\/core-instruments\/core-services\/\">Services page<\/a>.<br \/>\n<\/div>\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>The core facility is home to a variety of imaging instruments ranging from advanced laser scanning microscopes to ultrasound imaging systems. Though our central focus is supporting and using these instruments in neuroscience research, these techniques can be applied towards other aspects of biomedical imaging. On this page, you will find technical information about the [&hellip;]<\/p>\n","protected":false},"author":14840,"featured_media":0,"parent":3095,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/neurophotonics\/wp-json\/wp\/v2\/pages\/2211"}],"collection":[{"href":"https:\/\/www.bu.edu\/neurophotonics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bu.edu\/neurophotonics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/neurophotonics\/wp-json\/wp\/v2\/users\/14840"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/neurophotonics\/wp-json\/wp\/v2\/comments?post=2211"}],"version-history":[{"count":51,"href":"https:\/\/www.bu.edu\/neurophotonics\/wp-json\/wp\/v2\/pages\/2211\/revisions"}],"predecessor-version":[{"id":7827,"href":"https:\/\/www.bu.edu\/neurophotonics\/wp-json\/wp\/v2\/pages\/2211\/revisions\/7827"}],"up":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/neurophotonics\/wp-json\/wp\/v2\/pages\/3095"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/neurophotonics\/wp-json\/wp\/v2\/media?parent=2211"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}