RNA and DNA Extraction Protocols and Papers

This page will contain resources and open source links to RNA and DNA extraction methods for research. Will update regularly. This page will contain standard wet lab methods, kit protocols, and manuscripts describing novel and older techniques. Email catherin@bu.edu with additions/corrections.

Klapperich Lab Papers

Other Protocols

  • Chomczynski and Sacchi: Hard to spell, easier to do! Start here if you are thinking of trying out Boom on your own (Boom is what the Qiagen kits are based on). Chomczynski, P., Sacchi, N. The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nat Protoc 1, 581–585 (2006). https://doi.org/10.1038/nprot.2006.83 pdf
  • TRIzol, tried but true, for those who like efficient, don’t mind smelly, and are careful with pellets!
  • BOOM! The Boom Method. Can basically use any silica solid substrate for this one (including glass wool) – What Chomczynski is all about. open access link “Rapid and simple method for purification of nucleic acids.” Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J., J Clin Microbiol. 1990 Mar;28(3):495-503.
  • Phenol/Chloroform. For those who don’t want to pay full freight retail for TRIzol.



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