{"id":866,"date":"2013-06-02T23:34:23","date_gmt":"2013-06-03T03:34:23","guid":{"rendered":"https:\/\/www.bu.edu\/flow-cytometry\/?page_id=866"},"modified":"2020-12-16T10:21:46","modified_gmt":"2020-12-16T15:21:46","slug":"resources","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/flow-cytometry\/resources\/","title":{"rendered":"Resources"},"content":{"rendered":"
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<\/a><\/a>Instruments and services<\/h4>\n\n\n\n\n\n
BD LSRII SORP<\/td>\nBD LSR II User’s Guide<\/a> (by BD)
“Getting Started with BD FACSDiva Software”<\/a> (by BD)
“FACSDiva Reference Manual”<\/a> (by BD)
\nBD LSRII SORP data export\/import tutorial (FCCF)<\/a>
\nBiolegend Legendplex setup for the BD LSRII and BD FACSCanto<\/a><\/p>\n

BD LSRII SORP configuration (FCCF)<\/a>
\nLSRII Fluorophore laser\/filter\/PMT table<\/a><\/td>\n<\/tr>\n

Cytek Aurora 5L<\/td>\nEnabling and Optimizing High Quality Spectral Cytometry Analysis (BU Blackboard login required)<\/a>
\nCytek Aurora User’s Guide<\/a>
\nSpectraFlo Video Tutorials<\/a>
Cytek Biosciences resources page<\/a>\n<\/td>\n<\/tr>\n
BD FACSCalibur<\/td>\nBD FACSCalibur Guide<\/a> (by BD)
CELLQuest Pro User’s Guide<\/a> (BD)
CELLQuest Pro Acquisition Tutorial<\/a> (BD)
CELLQuest Pro Data Analysis Tutorial<\/a> (BD) <\/td>\n<\/tr>\n
Sorting<\/td>\n Practical Cell Sorting – A Guide for Investigators (UNC School of Medicine)<\/a>
Which data files should an investigator request after the sort? (FCCF)<\/a>\n<\/td>\n<\/tr>\n<\/table>\n

Ordering supplies<\/h4>\n\n\n\n\n\n\n
FCCF list of recommended tubes and reagents<\/td>\n<\/tr>\n
BD Biosciences<\/a><\/td>\nAntibodies, buffers and other flow cytometry reagents. BU users can order BD products through Fisher catalog to receive 10% discount and free shipping (don’t expect overnight shipping).<\/td>\n<\/tr>\n
Biolegend<\/a><\/td>\nAntibodies, buffers and other flow cytometry reagents. BU\/BMC users are eligible for up to 25-35% discount, please email us<\/a> for the quote code.<\/td>\n<\/tr>\n
Thermo Fisher (now owns Life Technologies\/Invitrogen\/Molecular Probes\/eBioscience)<\/a><\/td>\nFluorescent dyes, antibodies and other flow cytometry reagents. BU\/BMC users should order products through Terrier Marketplace\/Life Technologies catalog (NOT Fisher Scientific) to receive a discount (varies, 10-30%).<\/td>\n<\/tr>\n
Beckman Coulter<\/a><\/td>\nAntibodies<\/td>\n<\/tr>\n<\/table>\n

Online tutorials and courses<\/h4>\n\n\n\n\n\n\n
BD “Introduction to Flow Cytometry” <\/a><\/td>\nThis course is a prerequisite for FCCF New User Training<\/a><\/td>\n<\/tr>\n
BD Webinars<\/a><\/td>\nArchive of webinar recordings<\/td>\n<\/tr>\n
Cytek Biosciences<\/a><\/td>\nWebinars on spectral cytometry<\/td>\nWebinars on spectral cytometry<\/td>\n<\/tr>\n
Molecular Probes\/Invitrogen Webinars<\/a><\/td>\nArchive of webinar recordings<\/td>\n<\/tr>\n
FlowJo video tutorials<\/a>
FlowJo pdf tutorials and datasets<\/a><\/td>\n		Basic and advanced tutorials and sample data sets<\/td>\n<\/tr>\n<\/table>\n

Flow cytometry e-books<\/h4>\n\n\n\n\n
\n“Flow Cytometry – a Basic Introduction” by Michael G Ormerod (free e-book)<\/a><\/td>\n<\/tr>\n
“Practical Flow Cytometry” by Howard Shapiro (free e-book)<\/a><\/td>\n<\/tr>\n
Current Protocols In Cytometry (BU login required)<\/a><\/td>\n<\/tr>\n<\/table>\n

Web tools, spectrum viewers and fluorchrome charts<\/h4>\n\n\n\n\n\n\n\n
BD Biosciences Spectrum Viewer<\/a><\/td>\nFCCF’s favorite Spectrum Viewer for antibody-conjugated fluorochromes and common dyes and fluorescent proteins<\/td>\n<\/tr>\n
Thermo Fisher Spectraviewer<\/a> <\/td>\nIn addition to common conjugates, this viewer shows a great variety of dyes, proteins, pigments and probes<\/td>\n<\/tr>\n
Cytek Full Spectrum Viewer<\/a> <\/td>\nSpectral panel design tools including Similarity and Complexity Index calculator<\/td>\n<\/tr>\n
BD Relative Fluorochrome Brightness<\/a> <\/td>\nLists most recent BD dyes including Brilliant Violet and Brilliant UV Sirigen fluorochromes<\/td>\n<\/tr>\n
Fluorochrome chart<\/a> <\/td>\nLists most common fluorochromes, their excitation and emission and optimal filters sorted by brightness intensity<\/td>\n<\/tr>\n
Biolegend Instrument Guide\/Fluorophore Selector<\/a> <\/td>\nA great tool for listing colors for particular instruments\/configurations. <\/td>\n<\/tr>\n<\/table>\n

X3 Conference room<\/h4>\n\n\n
Room reservation calendar<\/a> (please email us<\/a> to have your event added)<\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Instruments and services BD LSRII SORP BD LSR II User’s Guide (by BD)”Getting Started with BD FACSDiva Software” (by BD)”FACSDiva Reference Manual” (by BD) BD LSRII SORP data export\/import tutorial (FCCF) Biolegend Legendplex setup for the BD LSRII and BD FACSCanto BD LSRII SORP configuration (FCCF) LSRII Fluorophore laser\/filter\/PMT table Cytek Aurora 5L Enabling and […]<\/p>\n","protected":false},"author":6872,"featured_media":0,"parent":0,"menu_order":6,"comment_status":"closed","ping_status":"closed","template":"blank.php","meta":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/flow-cytometry\/wp-json\/wp\/v2\/pages\/866"}],"collection":[{"href":"https:\/\/www.bu.edu\/flow-cytometry\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bu.edu\/flow-cytometry\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/flow-cytometry\/wp-json\/wp\/v2\/users\/6872"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/flow-cytometry\/wp-json\/wp\/v2\/comments?post=866"}],"version-history":[{"count":50,"href":"https:\/\/www.bu.edu\/flow-cytometry\/wp-json\/wp\/v2\/pages\/866\/revisions"}],"predecessor-version":[{"id":1751,"href":"https:\/\/www.bu.edu\/flow-cytometry\/wp-json\/wp\/v2\/pages\/866\/revisions\/1751"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/flow-cytometry\/wp-json\/wp\/v2\/media?parent=866"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}