{"id":38914,"date":"2022-12-01T12:23:02","date_gmt":"2022-12-01T16:23:02","guid":{"rendered":"https:\/\/www.bu.edu\/cise\/?post_type=research&#038;p=38914"},"modified":"2023-05-16T12:29:38","modified_gmt":"2023-05-16T16:29:38","slug":"bond-selective-intensity-diffraction-tomography","status":"publish","type":"research","link":"https:\/\/www.bu.edu\/cise\/research\/bond-selective-intensity-diffraction-tomography\/","title":{"rendered":"Bond-selective intensity diffraction tomography"},"content":{"rendered":"<div class=\"abstract\" id=\"abstract\">\n<div class=\"abstract-content selected\" id=\"eng-abstract\">\n<p>Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed a computational mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on a low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra and bond-selective 3D refractive index maps from intensity-only measurements. High-fidelity infrared fingerprint spectra extraction is validated. Volumetric chemical imaging of biological cells is demonstrated at a speed of ~20 s per volume, with a lateral and axial resolution of ~350 nm and ~1.1 \u00b5m, respectively. BS-IDT&#8217;s application potential is investigated by chemically quantifying lipids stored in cancer cells and volumetric chemical imaging on Caenorhabditis elegans with a large field of view (~100 \u00b5m x 100 \u00b5m).<\/p>\n<p>For more information, click <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36522316\/\" target=\"_blank\" rel=\"noopener noreferrer\">here<\/a>.<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Recovering molecular information remains a grand challenge in the widely used holographic and computational imaging technologies. To address this challenge, we developed a computational mid-infrared photothermal microscope, termed Bond-selective Intensity Diffraction Tomography (BS-IDT). Based on a low-cost brightfield microscope with an add-on pulsed light source, BS-IDT recovers both infrared spectra and bond-selective 3D refractive index [&hellip;]<\/p>\n","protected":false},"featured_media":0,"template":"","format":"standard","_links":{"self":[{"href":"https:\/\/www.bu.edu\/cise\/wp-json\/wp\/v2\/research\/38914"}],"collection":[{"href":"https:\/\/www.bu.edu\/cise\/wp-json\/wp\/v2\/research"}],"about":[{"href":"https:\/\/www.bu.edu\/cise\/wp-json\/wp\/v2\/types\/research"}],"version-history":[{"count":1,"href":"https:\/\/www.bu.edu\/cise\/wp-json\/wp\/v2\/research\/38914\/revisions"}],"predecessor-version":[{"id":38915,"href":"https:\/\/www.bu.edu\/cise\/wp-json\/wp\/v2\/research\/38914\/revisions\/38915"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/cise\/wp-json\/wp\/v2\/media?parent=38914"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}