{"id":22535,"date":"2021-01-12T09:02:00","date_gmt":"2021-01-12T14:02:00","guid":{"rendered":"https:\/\/www.bu.edu\/chemistry\/?p=22535"},"modified":"2021-01-12T09:02:00","modified_gmt":"2021-01-12T14:02:00","slug":"shu-wang-class-of-16-and-huijue-lyu-class-of-19","status":"publish","type":"post","link":"https:\/\/www.bu.edu\/chemistry\/2021\/01\/12\/shu-wang-class-of-16-and-huijue-lyu-class-of-19\/","title":{"rendered":"Shu Wang (Class of &#8217;16) and Huijue Lyu (Class of &#8217;19)"},"content":{"rendered":"<p><a href=\"https:\/\/doi.org\/10.1021\/acs.orglett.8b02332\">In Vitro Reconstitution of the Remaining Steps in Ovothiol A Biosynthesis: C\u2013S Lyase and Methyltransferase Reactions<\/a><\/p>\n<p style=\"text-align: justify;\"><span>Ovothiols are thiolhistidine derivatives. The first step of ovothiol biosynthesis is OvoA-catalyzed oxidative coupling between histidine and cysteine. In this report, the remaining steps of ovothiol A biosynthesis were reconstituted\u00a0<\/span><i>in vitro<\/i><span>. ETA_14770 (OvoB)\u00a0was reported as a PLP-dependent sulfoxide lyase, responsible for mercaptohistidine production. OvoA\u00a0was found to be a bifunctional enzyme, which mediates both oxidative C\u2013S bond formation and methylation of mercaptohistidine to afford ovothiol A. Besides reconstituting the whole biosynthetic pathway, two unique features proposed in the literature were also examined: a potential cysteine-recycling mechanism of the C\u2013S lyase (OvoB) and the selectivity of the \u03c0-<\/span><i>N<\/i><span>\u00a0methyltransferase.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>In Vitro Reconstitution of the Remaining Steps in Ovothiol A Biosynthesis: C\u2013S Lyase and Methyltransferase Reactions Ovothiols are thiolhistidine derivatives. The first step of ovothiol biosynthesis is OvoA-catalyzed oxidative coupling between histidine and cysteine. In this report, the remaining steps of ovothiol A biosynthesis were reconstituted\u00a0in vitro. ETA_14770 (OvoB)\u00a0was reported as a PLP-dependent sulfoxide lyase, [&hellip;]<\/p>\n","protected":false},"author":10766,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[9276,9277,9275,9274],"tags":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/posts\/22535"}],"collection":[{"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/users\/10766"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/comments?post=22535"}],"version-history":[{"count":1,"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/posts\/22535\/revisions"}],"predecessor-version":[{"id":22536,"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/posts\/22535\/revisions\/22536"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/media?parent=22535"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/categories?post=22535"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bu.edu\/chemistry\/wp-json\/wp\/v2\/tags?post=22535"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}