{"id":5572,"date":"2023-01-17T08:45:04","date_gmt":"2023-01-17T13:45:04","guid":{"rendered":"https:\/\/www.bu.edu\/bump\/?post_type=profile&p=5572"},"modified":"2023-01-17T12:05:28","modified_gmt":"2023-01-17T17:05:28","slug":"ilana-jacobs","status":"publish","type":"profile","link":"https:\/\/www.bu.edu\/bump\/people\/ilana-jacobs\/","title":{"rendered":"Ilana Jacobs"},"content":{"rendered":"

\"\"<\/p>\n

I hold a joint appointment at the Woods Hole Oceanographic Institute and Harvard University. I study ciliat<\/span><\/span>es found in anoxic and micro-oxic environments. Many eukaryotes in these conditions are known to be anaerobic respirators; Their\u00a0<\/span>Mitochondria have evolved to produce energy through pyruvate oxidation and substrate level phosphorylation (Rotterov\u00e1 et al., 2020), and produce hydrogen efficiently using [Fe-Fe]-hydrogenase via bacterial endosymbionts. We describe this type of Mitochondria as the Mitochondrial Related Organelle (MRO). In Dr. Colleen Cavanaugh’s lab, I am developing CRISPR\/Cas9- based genome editing protocol for an anaerobic ciliate, Heterometopus palaeformis<\/i> strain RAJCA, to better understand the functions of the MRO as well as identifying genes involved in the metabolic interdependency between the ciliate host and their endosymbionts. I am running transfection experiments and genotyping the transformed cells through DNA and RNA analysis (PCR, RT-PCR, transcriptome data preparation and analysis).<\/span>\u00a0This project is sponsored by Dr. Virginia Edgcomb and Dr. Fatma Gomaa at the Woods Hole Oceanographic Institute.<\/p>\n","protected":false},"author":2970,"template":"","_links":{"self":[{"href":"https:\/\/www.bu.edu\/bump\/wp-json\/wp\/v2\/profile\/5572"}],"collection":[{"href":"https:\/\/www.bu.edu\/bump\/wp-json\/wp\/v2\/profile"}],"about":[{"href":"https:\/\/www.bu.edu\/bump\/wp-json\/wp\/v2\/types\/profile"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/bump\/wp-json\/wp\/v2\/users\/2970"}],"version-history":[{"count":1,"href":"https:\/\/www.bu.edu\/bump\/wp-json\/wp\/v2\/profile\/5572\/revisions"}],"predecessor-version":[{"id":5574,"href":"https:\/\/www.bu.edu\/bump\/wp-json\/wp\/v2\/profile\/5572\/revisions\/5574"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/bump\/wp-json\/wp\/v2\/media?parent=5572"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}