{"id":1036,"date":"2021-04-16T13:35:55","date_gmt":"2021-04-16T17:35:55","guid":{"rendered":"https:\/\/www.bu.edu\/bif\/?p=1036"},"modified":"2021-04-16T13:35:55","modified_gmt":"2021-04-16T17:35:55","slug":"c2-confocal-recent-issue-and-warning","status":"publish","type":"post","link":"https:\/\/www.bu.edu\/bif\/2021\/04\/16\/c2-confocal-recent-issue-and-warning\/","title":{"rendered":"C2 Confocal -recent issue and WARNING"},"content":{"rendered":"\r\n

The results of your feedback and a bit of forensic analysis have led to the following post-issue response. Background: on 4\/11 two of the insert securing screws sheared off during operation.
Although in the past, using an incorrect technique when changing inserts stripped the screws, that was not involved in the current issue. The screws behaved as expected in a safety role. The 40x water immersion objective was striking the stage (see below).
Please make certain that you are not trying to focus beyond the capability of the system. Slides must be coverslip down and any specialized chambers should be closely monitored to make sure there is no contact. Checking the reported Z height when focusing is a good way to make sure that you are moving in the correct direction and you aren\u2019t beyond the safe travel range.

\"\"<\/a> \"\"<\/a> \"\"<\/a> \"\"<\/a><\/p>\r\n","protected":false},"excerpt":{"rendered":"

The results of your feedback and a bit of forensic analysis have led to the following post-issue response. Background: on 4\/11 two of the insert securing screws sheared off during operation.Although in the past, using an incorrect technique when changing inserts stripped the screws, that was not involved in the current issue. The screws behaved […]<\/p>\n","protected":false},"author":3474,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/posts\/1036"}],"collection":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/users\/3474"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/comments?post=1036"}],"version-history":[{"count":2,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/posts\/1036\/revisions"}],"predecessor-version":[{"id":1043,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/posts\/1036\/revisions\/1043"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/media?parent=1036"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/categories?post=1036"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/tags?post=1036"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}