{"id":757,"date":"2019-05-28T15:27:07","date_gmt":"2019-05-28T19:27:07","guid":{"rendered":"https:\/\/www.bu.edu\/bif\/?page_id=757"},"modified":"2025-06-18T14:43:13","modified_gmt":"2025-06-18T18:43:13","slug":"sapphire-specs-and-methods","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/bif\/imaging\/sapphire-specs-and-methods\/","title":{"rendered":"Sapphire specs and methods"},"content":{"rendered":"<h1>Fluorescence Mode<\/h1>\n<p>Our system has the ability to image fluorescence excited with 488nm 520nm 658nm or 784nm laser lines<\/p>\n<p>Any signal emitted from the sample can be collected either simultaneously or sequentially (NEW!) on four PMT channels<\/p>\n<p style=\"padding-left: 30px;\">Note : filters are listed as the center of passband and width in nm so 518bp 22 would allow 509nm- 529nm to pass through to the detector<\/p>\n<ul>\n<li>\n<h2><span style=\"color: #00ffff;\">blue<\/span> 518BP22<\/h2>\n<\/li>\n<li>\n<h2><span style=\"color: #339966;\">green<\/span> 565BP24<\/h2>\n<\/li>\n<li>\n<h2><span style=\"color: #ff0000;\">Red<\/span> 710BP40<\/h2>\n<\/li>\n<li>\n<h2>IR 823BP37<\/h2>\n<\/li>\n<\/ul>\n<h1>Storage Phosphor Mode<\/h1>\n<p>Dedicated laser and PMT settings to activate and detect signals stored in a phosphor screen that was exposed to a radiolabeled sample<\/p>\n<p>&nbsp;<\/p>\n<h1>Chemiluminescence Imaging<\/h1>\n<p>A cooled CCD camera is used to detect ECL, Luminol, or other chemiluminescent substrates.\u00a0 This can be used in place of x-ray film and XOMAT development.<\/p>\n<p>Note that only the top half of the scanner platen can be imaged with the CCD camera.<\/p>\n<h1>Visible<\/h1>\n<p>The area of the platen marked with the blue stripe and &#8216;chemi&#8217; is also appropriate for Visible imaging of colorimetric signals in gels and blots. Silver stains, <span class=\"module__title__link\">Coomassie, ponceau red etc.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h1>Appropriate samples<\/h1>\n<p>Multiple types of samples can be imaged.\u00a0 The sample type pull down corresponds to some common types of samples<\/p>\n<ul>\n<li>Membrane (0 mm)\u00a0 The focal plane is on the glass<\/li>\n<li>Gel +0.5mm\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 0.5mm above the glass<\/li>\n<li>Plate +3.0mm<\/li>\n<li>Slide +1 mm\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 1.0mm above the glass for CS imaging<\/li>\n<li>Custom\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 User-selectable through 4mm of travel<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>The customized user manual for our Sapphire scanner can be downloaded here: <a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/PICFSapphire-User-Manual.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">PICFSapphire User Manual<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>Are you looking for the calendar for the sapphire? Here<\/p>\n<h1>Methods and Notes<\/h1>\n<ol>\n<li><a href=\"\/bif\/files\/2019\/05\/Azure-Western-Blotting-Guidebook.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">A complete overview of the offerings and options for Westerns: Western-Blotting-Guidebook.pdf<\/a><\/li>\n<li class=\"attachment\"><a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/Wet-or-Dry-app_3.pdf\">Wet-or-Dry-app_3<\/a><\/li>\n<li class=\"attachment\"><a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/Three-Color-Western-Blots-with-AzureSpectra-Antibodies.pdf\">Three-Color-Western-Blots-with-<\/a><\/li>\n<li class=\"attachment\"><a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/Improve-Chemi-Blots-app-note_3.pdf\">Improve-Chemi-Blots-app-note_3<\/a><\/li>\n<li class=\"attachment\"><a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/Three-Color-Western-Blots-with-AzureSpectra-Antibodies.pdf\">AzureSpectra-Antibodies<\/a><\/li>\n<li class=\"attachment\"><a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/Improve-Fluorescent-Blots-app-note_3.pdf\">Improve-Fluorescent-Blots-app-note_3<\/a><\/li>\n<li class=\"attachment\"><a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/App-Note-Total-Protein-Normalization-with-AzureRed_10-002.pdf\">App-Note-Total-Protein-Normalization-with-AzureRed_10-002<\/a><\/li>\n<li class=\"attachment\"><a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/App-Note-Phosphor-Imaging-with-Sapphire-Biomolecular-Imager.pdf\">App-Note-Phosphor-Imaging-with-Sapphire-Biomolecular-Imager<\/a><\/li>\n<li class=\"attachment\"><a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/App-Note-Blocking_1.pdf\">App Note-Blocking_1<\/a><\/li>\n<li class=\"attachment\"><a href=\"https:\/\/www.bu.edu\/bif\/files\/2019\/05\/App-Note-In-cell-Western-blotting_4.pdf\">App-Note-In-cell-Western-blotting_4<\/a><\/li>\n<li class=\"post-title\"><a href=\"https:\/\/www.bu.edu\/bif\/app-note-increasing-assay-efficiency-with-four-color-detection_4\/\">App-Note-Increasing-Assay-Efficiency-with-Four-Color-Detection_4<\/a><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Fluorescence Mode Our system has the ability to image fluorescence excited with 488nm 520nm 658nm or 784nm laser lines Any signal emitted from the sample can be collected either simultaneously or sequentially (NEW!) on four PMT channels Note : filters are listed as the center of passband and width in nm so 518bp 22 would [&hellip;]<\/p>\n","protected":false},"author":3474,"featured_media":0,"parent":141,"menu_order":9,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages\/757"}],"collection":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/users\/3474"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/comments?post=757"}],"version-history":[{"count":8,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages\/757\/revisions"}],"predecessor-version":[{"id":1070,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages\/757\/revisions\/1070"}],"up":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages\/141"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/media?parent=757"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}