{"id":563,"date":"2017-05-11T14:55:38","date_gmt":"2017-05-11T18:55:38","guid":{"rendered":"https:\/\/www.bu.edu\/bif\/?page_id=563"},"modified":"2025-06-18T14:43:12","modified_gmt":"2025-06-18T18:43:12","slug":"sony-sh800-cell-sorter","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/bif\/imaging\/sony-sh800-cell-sorter\/","title":{"rendered":"Sony SH800 Cell Sorter"},"content":{"rendered":"

We have a Sony SH800 cell sorter that can accept 1.5mL microfuge tubes, 5mL FACS tubes, 15mL centrifuge tube samples and sort cells into 5mL FACS tubes, 15mL tubes centrifuge\u00a0tubes, 96 and 384 well plates.\u00a0 The system bills at the rate of \u00a0$90\/hour (BU\/BMC), $120\/hour (external academic) and $160\/hour (external commercial). Currently, there is only staff operation. \u00a0 Sorting is not performed on demand and must be scheduled in advance. Email (blute\u00a0<\/strong>at bu<\/strong> dot edu<\/strong>)\u00a0or call 617 353-2638<\/p>\n

 <\/p>\n

\"Capturesh800spectra\"<\/p>\n

 <\/p>\n

Specs – Spreadsheet4<\/a><\/p>\n\n\n\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Optics<\/td>\nExcitation lasers<\/td>\n488 nm, 405 nm, 638 nm and 561 nm laser<\/td>\n<\/tr>\n
Output power<\/td>\n30 mW (max.) optical fiber output<\/td>\n<\/tr>\n
Beam alignment<\/td>\nCollinear optical system<\/td>\n<\/tr>\n
Detection Parameters<\/td>\n6 fluorescent + 2 scatter<\/td>\n<\/tr>\n
Analog-to-digital converters (ADC)<\/td>\n8-channel 20-bit, 110MHz<\/td>\n<\/tr>\n
Pulse measurement<\/td>\nHeight, Area, Width<\/td>\n<\/tr>\n
Fluidics<\/td>\nSample tube<\/td>\nSingle, auto-loading tube<\/td>\n<\/tr>\n
Tube types<\/td>\n\u00a01.5 ml, 5 ml and 15 ml tubes<\/td>\n<\/tr>\n
Sort Devices<\/td>\n2-way tube, multiwell plates,<\/td>\n<\/tr>\n
Temperature control<\/td>\n5\u00b0C, 37\u00b0C (Electric Cooling Method)<\/td>\n<\/tr>\n
Agitation unit<\/td>\nEccentric rotation<\/td>\n<\/tr>\n
Magnetic drive<\/td>\n300 rpm speed<\/td>\n<\/tr>\n
Sorting chip size<\/td>\n70 \u03bcm, 100 \u03bcm and 130 \u03bcm<\/td>\n<\/tr>\n
Sort Performance<\/td>\nEvent rate<\/td>\n100,000 eps<\/td>\n<\/tr>\n
Sorting Speed<\/td>\nUsing the 70 \u03bcm sorting chip at 50 kHz an average threshold of 12,000 events per second can be achieved with >95% purity and >80% yield. The threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson\u2019s statistics.<\/td>\n<\/tr>\n
Scatter resolution<\/td>\n0.5\u03bcm<\/td>\n<\/tr>\n
Fluorescence resolution<\/td>\n< 2.5% Half-peak coefficient of variation (HPCV)<\/td>\n<\/tr>\n
Fluorescence sensitivity<\/td>\nFITC 120 MESF, PE 110 MESF<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\n
\n
\n
\n
\n
\n

\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

We have a Sony SH800 cell sorter that can accept 1.5mL microfuge tubes, 5mL FACS tubes, 15mL centrifuge tube samples and sort cells into 5mL FACS tubes, 15mL tubes centrifuge\u00a0tubes, 96 and 384 well plates.\u00a0 The system bills at the rate of \u00a0$90\/hour (BU\/BMC), $120\/hour (external academic) and $160\/hour (external commercial). Currently, there is only […]<\/p>\n","protected":false},"author":3474,"featured_media":0,"parent":141,"menu_order":7,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages\/563"}],"collection":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/users\/3474"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/comments?post=563"}],"version-history":[{"count":12,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages\/563\/revisions"}],"predecessor-version":[{"id":600,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages\/563\/revisions\/600"}],"up":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/pages\/141"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/bif\/wp-json\/wp\/v2\/media?parent=563"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}