{"id":480,"date":"2016-10-04T14:09:23","date_gmt":"2016-10-04T18:09:23","guid":{"rendered":"https:\/\/www.bu.edu\/bif\/?page_id=480"},"modified":"2025-06-20T11:32:27","modified_gmt":"2025-06-20T15:32:27","slug":"other-equipment","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/bif\/other-equipment\/","title":{"rendered":"Other Equipment"},"content":{"rendered":"

This is the landing page for other equipment that could benefit from a schedule. \u00a0It will also be a repository for pertinent methods, protocols, and songs of the old ones.<\/p>\n

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Agilent 2100 BioAnalyzer<\/h2>\n

The Windows 10 64 bit system has ample RAM and video memory for most tasks. The credentials used for access are posted on the monitor.\u00a0 In the future, the system may transition to individual Kerberos logins but for now please use the local ‘BioAnalyzer’ account.<\/p>\n

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There is a NanoDrop OneC (c is for cuvette) in LSE 632<\/p>\n

Here is the complete Users guideNanoDrop One_User_Guide _for v1.3 sw_ENGLISH<\/a> for this very versatile spectrophotometer<\/p>\n

Also a performance verification white paper<\/a> on A205 nm methods<\/p>\n

The NanoDropOneC viewer software for offline analysis of your data can be downloaded from this link<\/a><\/p>\n

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Leica Sliding microtome in LSE 452(below)<\/p>\n

\"SlidingLeica SM 2000R freezing stage sliding microtome<\/strong> has been added to the schedule as ‘sliding microtome’.<\/p>\n

General notes on it are as follows;<\/p>\n

NEVER dispose of dry ice in the sink. \u00a0It can destroy the pipes. \u00a0Just wait it out it will sublimate on its own. \u00a0Also, alcohol cannot be disposed of in the sink. \u00a0There is a large red carboy on the floor next to the fume hood for disposing of unwanted ethanol. \u00a0 The blades are EXTREMELY sharp. \u00a0Always exercise caution when loading, removing and cleaning the blade. \u00a0While they are expensive if you drop it, get away and LET IT FALL. \u00a0There are numerous permanent blades that you may use. \u00a0If you find\u00a0a blade that is dull, dirty or damaged please let me know so that it can be addressed. \u00a0You are welcome to bring your own blades or use one that we provide. \u00a0The manual for it can be downloaded from\u00a0Leica_SM2000R_Manual<\/a>. \u00a0Please note that at this time due to high demand the SM2000R is only for cutting frozen material. \u00a0Any Paraffin workflow are cut on the Leica RM2145 rotary microtome<\/p>\n

A rotary microtome for sectioning paraffin and JB4 blocks is available in LSE 452B<\/p>\n

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The Leica rotary microtome RM2145 is on the scheduler here. \u00a0The users manual for this particular wax microtome can be found here<\/a><\/p>\n

The Leica RM2145<\/b> is a semi-motorized rotary microtome manufactured especially for paraffin specimens and\u00a0is equipped with an automated coarse specimen advance feature. \u00a0Along with a single-handed quick release specimen clamping system and programmable specimen retraction, the Leica RM2145 microtome<\/b> offers a clear LED display of section thickness and section count. Additionally, this Leica microtome has a motorized coarse advance operation using a rocker button, a fast and safe specimen orientation with anti-tilt function, and a universal knife holder.<\/p>\n

Here is a good video reminder of the basics of sectioning.<\/p>\n