{"id":740,"date":"2023-06-09T09:38:30","date_gmt":"2023-06-09T13:38:30","guid":{"rendered":"https:\/\/www.bu.edu\/becf\/?page_id=740"},"modified":"2023-11-03T14:21:08","modified_gmt":"2023-11-03T18:21:08","slug":"csu-w1-sora","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/becf\/micronano-imaging-mni-facility\/csu-w1-sora\/","title":{"rendered":"Nikon CSU-W1 SoRA Spinning Disk Confocal Microscope"},"content":{"rendered":"\n\n\n\n
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Invitrogen, BPAE Cells, DAPI, Alex488, Mitotracker Red cells imaged using (Left) 20x, 0.7 NA\u00a0objective and\u00a0(Right) 60x, 1.42 NA oil immersion lens with SoRa disk, 4x auxiliary magnification,\u00a0and Lucy-Richardson deconvolution.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

CSU-W1 SoRa is a NEWLY acquired dual camera spinning disk (inverted) confocal microscope from Nikon, located in the Life Science and Engineering Building<\/a> (LSEB 222). The confocal imaging platform uses two high-speed Yokogawa spinning disks with series of pinholes to reject out-of-focal plane background signal, providing high quality confocal images at frame rates up to 200 Hz. The\u00a0CSU-W1 disk has a new pinhole\u00a0design with optimal pinhole size and spacing, significantly reducing the pinhole crosstalk – an important feature when imaging thicker samples with multiple scattering. The\u00a0<\/span><\/span> super resolution SoRa disk has additional lenslet array. With high NA objectives and auxiliary magnification, higher quality images with spatial resolution 1.4\u00a0<\/span>the optical limit is possible. Using deconvolution, imaging\u00a0resolution of CSU-W1 SoRa system can be further increased up to 2x.<\/p>\n

Microscope features:<\/strong><\/h4>\n