{"id":207,"date":"2019-05-06T11:01:53","date_gmt":"2019-05-06T15:01:53","guid":{"rendered":"https:\/\/www.bu.edu\/becf\/?page_id=207"},"modified":"2023-08-31T11:24:25","modified_gmt":"2023-08-31T15:24:25","slug":"vs120-2","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/becf\/micronano-imaging-mni-facility\/vs120-2\/","title":{"rendered":"Olympus VS120 Virtual Slide Scanner"},"content":{"rendered":"<p style=\"padding-left: 30px;\">The VS120 is a high-throughput slide scanner that is capable of automatically imaging and stitching 100 slides in <strong>color bright field mode<\/strong> as well as<strong> fluorescence mode<\/strong>.<\/p>\n<p style=\"padding-left: 30px;\">The automated slide scanner is equipped with 10x, 20x, 40x objectives, a 3.2MP colored camera (AVT Pike F-505C) for color imaging, and a Hamamatsu Orca Flash 4.0 for fluorescence imaging with fluorescence emission\/excitation filter cubes for the following stains: DAPI, FITC, TRITC, CY5 and CY7 dyes. An X-Cite 120LED light engine from Excelitas is used for fluorescence excitation, to ensure minimum system maintenance.<\/p>\n<p>&nbsp;<\/p>\n<h2>Other Resources<\/h2>\n<h3 style=\"padding-left: 30px;\">Quick Start Guide<\/h3>\n<p style=\"padding-left: 30px;\">Here is an <a href=\"\/becf\/files\/__restricted\/entire-bu-community\/Olympus-VS120-Quick-Start-rev-1.0.pdf\">Olympus VS120 Quick Start (rev 1.0)<\/a> guide that the MNI Facility wrote for this instrument.<\/p>\n<p style=\"padding-left: 30px;\">Here is the manual for this <a href=\"\/becf\/files\/2020\/02\/olympus_vs_asw_imaging_software_manual.pdf\">Olympus VS120 Manual<\/a>.<\/p>\n<h3 style=\"padding-left: 30px;\">Video Tutorials<\/h3>\n<p style=\"padding-left: 30px;\">Our friends at UT Dallas has made excellent tutorial videos on how to operate the Olympus VS120. <a href=\"https:\/\/research.utdallas.edu\/researchers\/core-facilities\/imaging-and-histology-core\/microscopes\">Click here<\/a> to be redirected to their website to view their videos.<\/p>\n<p style=\"padding-left: 30px;\">Here are the direct Youtube videos.\u00a0Don&#8217;t forget to &#8220;like&#8221; their work.<\/p>\n<div class=\"responsiveVideo responsive-youtube\"><iframe loading=\"lazy\" title=\"Module 1 Introduction and Bright Field Imaging\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/KISaMwEophI?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" allowfullscreen><\/iframe><\/div>\n<div class=\"responsiveVideo responsive-youtube\"><iframe loading=\"lazy\" title=\"Module 2 Fluorescence Imaging\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/pDQpA99UYG8?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" allowfullscreen><\/iframe><\/div>\n<h3 style=\"padding-left: 30px;\">Olympus Image Format Plugin for ImageJ\/Fiji<\/h3>\n<p style=\"padding-left: 30px;\">If you wish to load your saved files into ImageJ, <a href=\"https:\/\/imagej.net\/OlympusImageJPlugin\">click here<\/a> to download the ImageJ\/Fiji plugin. Once you have downloaded it, please install it like any other plugin for ImageJ and reload ImageJ.<\/p>\n<h3 style=\"padding-left: 30px;\">Olympus Viewer 3<\/h3>\n<p style=\"padding-left: 30px;\">Olympus offers free viewing software for the VS120. Please visit\u00a0<a href=\"https:\/\/www.olympus-lifescience.com\/en\/support\/downloads\/\">https:\/\/www.olympus-lifescience.com\/en\/support\/downloads\/<\/a> and download the latest version of OlyVIA to view your slide images. You can find the OlyVIA software under VIRTUAL SLIDE MICROSCOPES. Please download the VS200 software, which is compatible with the image files generated by the VS120 and allow you to export the images into more common formats such as TIFF&#8217;s and JPG&#8217;s.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The VS120 is a high-throughput slide scanner that is capable of automatically imaging and stitching 100 slides in color bright field mode as well as fluorescence mode. The automated slide scanner is equipped with 10x, 20x, 40x objectives, a 3.2MP colored camera (AVT Pike F-505C) for color imaging, and a Hamamatsu Orca Flash 4.0 for [&hellip;]<\/p>\n","protected":false},"author":14292,"featured_media":0,"parent":24,"menu_order":7,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.bu.edu\/becf\/wp-json\/wp\/v2\/pages\/207"}],"collection":[{"href":"https:\/\/www.bu.edu\/becf\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bu.edu\/becf\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/becf\/wp-json\/wp\/v2\/users\/14292"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/becf\/wp-json\/wp\/v2\/comments?post=207"}],"version-history":[{"count":14,"href":"https:\/\/www.bu.edu\/becf\/wp-json\/wp\/v2\/pages\/207\/revisions"}],"predecessor-version":[{"id":797,"href":"https:\/\/www.bu.edu\/becf\/wp-json\/wp\/v2\/pages\/207\/revisions\/797"}],"up":[{"embeddable":true,"href":"https:\/\/www.bu.edu\/becf\/wp-json\/wp\/v2\/pages\/24"}],"wp:attachment":[{"href":"https:\/\/www.bu.edu\/becf\/wp-json\/wp\/v2\/media?parent=207"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}