{"id":201,"date":"2019-05-06T11:00:39","date_gmt":"2019-05-06T15:00:39","guid":{"rendered":"https:\/\/www.bu.edu\/becf\/?page_id=201"},"modified":"2023-06-09T08:45:44","modified_gmt":"2023-06-09T12:45:44","slug":"fv3000-2","status":"publish","type":"page","link":"https:\/\/www.bu.edu\/becf\/micronano-imaging-mni-facility\/fv3000-2\/","title":{"rendered":"Olympus FV3000 Scanning Confocal Microscope"},"content":{"rendered":"

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Olympus FV3000 Inverted Confocal Microscope<\/td>\n

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Invitrogen, BPAE Cells, DAPI, Alex488, Mitotracker Red<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Olympus FV3000<\/strong> is laser scanning confocal microscope to\u00a0obtain high-resolution, high-contrast images of a sample. It scans samples point by point, resulting in precise 3D\u00a0fluorescence images.
\n– 4x Fluorescence detectors, two bi-metal alkali PMT and two high sensitivity GaAsP\/GaAs PMT detectors.
\n– 1x bi-metal Alkali PMT transmission detector for DIC Imaging
\n– High Efficiency Spectral Detection using Volume Holographic Gratings
\n– Conventional XY Glavo and High-speed Resonant Galvo-Galvo Scanning
\n– Motorized XYZ stage for easy transition between macroscopic to microscoping imaging
\n– 6x solid state diode lasers (405nm, 445nm, 488nm, 514nm, 561nm, 640nm)
\n– 6x Olympus Objectives
\ni.) PLAPON, 1.25x, 0.04 NA, 5mm WD
\nii.) UPLSAPO10X2, 10x, 0.4NA, 3mm WD
\niii.) UPLSAPO20X, 20x, 0.75NA, 0.6mm WD
\niv.) LUCPLFLN20X, 20x, 0.45NA, 6.60mm-7.80mm WD
\nv.)\u00a0PLAPON60XO, 60x, 1.42 NA, 0.15mm WD (oil dipped)
\nvi.)\u00a0UPLSAPO100XS, 100x, 1.35 NA, 0.2mm WD (Si oil dipped)<\/p>\n

– Fully enclosed environmental chamber for live cell imaging
\n– Z Drift Compensation for tracking focal plane shifts during long duration cell imaging trials
\n– Specialized holders for slides, wellplates, and petri dishes
\n– CellSense Deconvolution Solution for PSF deconvolution<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n

Acknowledgement Requirements<\/h2>\n

Users who have gathered data using this microscope\u00a0must<\/strong> include the following statement in their acknowledgement for any paper, poster and oral presentation.<\/p>\n

“Research reported in this publication was supported by the Boston University Micro and Nano Imaging Facility and the Office of the Director, National Institutes of Health of the National Institutes of Health under award Number S10OD024993. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Health.”<\/em><\/p>\n

Other Resources<\/strong><\/h2>\n

Quick-Start Guide<\/h3>\n

Click here to access the Olympus FV3000 Operating Manual Guide<\/a>. (BU Affiliated members Only)
\nClick here to access the FV3000 Training Notes REV AD<\/a>. (BU Affiliated members Only)<\/p>\n

Objective Reservation<\/h3>\n

Click here<\/a> to request access to the 60x or 100x objectives.<\/p>\n

Video Tutorials<\/h3>\n

Our friends at the UT Dallas has produced some very informative videos on the FV3000. Please visit them at here<\/a> and like their videos to recognize their work.<\/p>\n

Here are their direct links to Youtube.<\/p>\n