PI – Jag Bhawan, MD
|JungEun (Joanna) Leefirstname.lastname@example.org|
Dermatopathology Fee Schedule
|Embedding formalin-fixed skin||Sectioning paraffin block||H&E Stain||IHC Stain|
|Human sample associated with MRSS/clinical data;
sample remainder donated to core for future use.
|No cost||Up to 5 unstained slides
at no cost
|Up to 1 at
|Up to 1 at
|Human sample not associated with MRSS/clinical data;
not being donated for future use.
Antibodies Currently Available for Immunohistochemical (IHC) Staining
|Smooth Muscle Actin||1A4||Dako|
|Von Willebrand Factor||F8/86||Dako|
|P300||C-20||Santa Cruz Biotechnology|
We can also work with any other antibodies not listed to develop staining protocols.
This core will provide uniform processing of skin samples from various investigators. We have a well established method of preparing 8 skin samples in one block to save costs as well as avoid variability of staining between samples. We have state of the art automated equipment for routine histopathology and immunopathology needs. The lab is equipped with a photomicroscope with a digital camera which can make excellent photomicrographs. Our image analysis system can evaluate various parameters in an objective manner.
In addition to routine histopathology, immunostaining with any antibody can be performed. The director has tremendous experience with various antibodies including SMA, CD31, lymphocyte markers, CD34, and cathepsin k, most relevant to this field.
a. Process and stain pathologic specimens. 8-sample skin arrays analyzed by histochemistry or IHC for novel proteins, and connective tissues, vascular and immune markers per request of Core Investigators.
b. Evaluate and interpret staining.
Histochemical or immunohistochemical staining intensity will be evaluated using semi-quantitative methods or by image analysis for quantification of staining. Other relevant clinical information including autoantibodies, duration of disease, etc. will be available and used for correlation studies.
Develop a database of staining. A database of staining patterns (cell numbers or intensities) will be developed for correlating changes across studies
Develop new technologies. We will develop microdissection followed by microarray
Click here to view the Skin Pathology Standard Operating Procedures