sizereqs

Isotopes	Sample Matrix	Volume/Mass

Doubly-labeled Water (metabolic studies):
¹⁸O	body fluid	200ul*
HD	body fluid	10ul*
Other enriched water studies
¹⁸O	plant or soil fluid	200ul*
HD	plant or soil fluid	10ul*
Natural Abundance Fluid/Water:
¹⁸O	fluid	200ul
HD	fluid	10ul
C and N	plant tissue	5-10 mg (C)
		30-50 mg (N)
	dried animal tissue	2-5 mg (C)
		2-10 mg (N)
	sediment (marine, freshwater)	2-10 mg(C)
		50 mg (N)
	soil (varies with %C)	20-1000 mg
	water for NH₄, NO₃	500-1000 ml
	water samples for DIC	200 ml
	blood for bicarbonate	1 ml
	S dried animal tissue	50 mg
	dried plant tissue	100 mg
	sediment and soil	100 mg
	water samples	1 liter

* a total of 210 ul of fluid is needed. 18O for smaller samples is under development. For one analysis, 100 ul is currently required.