Professor DeRosa Presents at The Center for the Advancement of STEM Education Conference at Bridgewater University
Professor Don DeRosa is invited to present at The Center for the Advancement of STEM Education (CASE) Conference at Bridgewater University on Thursday, February 27. Professor DeRosa will lead a teacher workshop with Dr. Carla Romney of Boston University entitled Glow Sticks: Affinity Chromatography for Protein Purification.
Below is a brief description of the workshop:
Green fluorescent protein (GFP) is a popular choice for teaching about proteins and protein chemistry. While hydrophobic interaction chromatography is often used to purify GFP, Immobilized metal ion affinity chromatography (IMAC) is more efficient and results in purer yields. The workshop will highlight a modified IMAC protocol used in the CityLab SummerLab biotechnology program for high school students directed by DeRosa and Romney. In this hands-on workshop, participants will lyse E.coli bacteria that have been transformed to produce histidine-tagged GFP and learn how the histidine tag is used to purify GFP using IMAC.
Visit the CASE Conference website to learn more.