Laboratory Animal Science Center (BUMC)
Rodent Health Surveillance & Bioexclusion Profile
List of excluded agents: (A= annual testing; 2X/yr; Q = quarterly testing)
| Agent Type | Agent Name | Rats | Mice |
|---|---|---|---|
| Viruses | Ectromelia virus (ECTRO) of mice | 2X/yr | |
| Epizootic diarrhea of infant mice (EDIM) | Q | ||
| GD VII (TMEV) | A | Q | |
| Hantaan virus (HANT) | A | 2X/yr | |
| Kilham rat virus (KRV) | Q | ||
| Lactate Dehydrogenase-elevating virus (LDV) | A | ||
| Lymphocytic choriomeningitis virus (LCMV) | A | 2X/yr | |
| Minute virus of mice (MVM) | Q | ||
| Mouse adenovirus (MAV 1&2) | A | 2X/yr | |
| Mouse cytomegalovirus (MCMV) | 2X/yr | ||
| Mouse hepatitis virus (MHV) | Q | ||
| Mouse norovirus (MNV) | Q | ||
| Mouse parvovirus (MPV) | Q* | ||
| Mouse pneumonitis (K virus) | 2Xyr | ||
| Mouse thymic virus (MTLV) | 2X/yr | ||
| Parvovirus NS1 | Q | Q | |
| Pneumonia virus of mice (PVM) | Q | Q | |
| Polyoma virus (POLY) | 2X/yr | ||
| Rat minute virus (RMV) | Q | ||
| Rat parvovirus (RPV) | Q | ||
| Rat respiratory virus (RRV - histology) | 2x/yr | ||
| Reovirus (REO) | A | Q | |
| Sendai virus (SEN) | Q | Q | |
| Sialodacryoadenitis virus (SDA/RCV) | Q | ||
| Toolan's H-1 virus (H-1) | Q | ||
| Parasites | Syphacia muris | 2x/yr | |
| Syphacia obvelata | 2x/yr | ||
| Aspiculuris tetraptera | 2x/yr | ||
| Encephalitozoon cuniculi (ECUN) | A | A | |
| Fur mites | 2x/yr | 2x/yr | |
| Lice | 2x/yr | 2x/yr | |
| Bacteria | CAR bacillus | A | 2X/yr |
| Citrobacter freundii | A | A | |
| Corynebacterium kutscheri | A | A | |
| Salmonella spp. | A | A | |
| Streptobacillus moniliformis | A | A | |
| Mycoplasma pulmonis (MPUL) | Q | Q | |
| Gross Pathological Examination | A | A | |
Agents that are present in existing colonies, but that we preferentially exclude from incoming shipments (we request agent-free animals as the first choice, but will accept animals infected with these agents:
- Bordetella bronchiseptica
- Selected Helicobacter species (excluded from 670 and W9 barrier facilities, permitted in other facilities)
- Klebsiella pneumonia and K. oxytoca
- Pasteurella. species
Agents that we do not exclude from incoming vendors:
- Pasteurella spp (other than P. multocida and P. pneumotropica)
- Pseudomonas aeruginosa
- Staphylococcus aureus
- Streptococcus pneumoniae
- Beta Strep spp (including Group B and Group G)
Testing of immunodeficient rodents Immunodeficient rodents require adjustments in testing, since they do not develop serum antibodies. These may include use of contact sentinels and virus testing.
*Testing during enzootics The LASC reserves the right to test more frequently than stated above during enzootics at the discretion of the Veterinary Staff. For example, during the present MPV enzootic, mice are tested for MPV every 6 weeks.
Note: We restrict severely immunodeficient animals (scids, nudes) to barriers free of all the above agents plus Pneumocystis carinii.