Imaging

The facility has numerous microscopes with different capabilities located throughout the Imaging suite and the department.

LSEB 448- Confocal imaging suite

This suite currently houses 4 confocal microscope rigs, 2 data analysis stations   as well as our transmission electron microscope .

ALL OF THIS EQUIPMENT REQUIRES TRAINING PRIOR TO USE

The first bay houses an inverted Olympus IX70 inverted microscope with epi-fluorescence and a ccd camera.  As well as a data analysis work station

neither of these are on the core scheduler at this time nor is there a per use charge.

The second system is a Fluoview 300 confocal laser scanning microscope located in the second bay(from the left) of 448

In the core scheduler this is Fluoview 1

This system bills at a rate of $9.00 hr internal academic/$15.00 hr external academic

SOP and information for Fluoview 1(coming soon)

The third bay is home to an Olympus DSU spinning disk confocal.

In the core scheduler it is Olympus DSU(LSEB)

This system bills at a rate of $9.00 hr internal academic/$15.00 hr external academic

SOP and information for DSU(coming soon)

The fourth bay is home to our newest confocal, an Olympus Fluoview FV10i inverted self-contained confocal laser scanning system.

It is excruciatingly popular and in the core scheduler it is FV10i

This system bills at a rate of $9.00 hr internal academic/$15.00 hr external academic

Data generated on the 10i can be analyzed with an offline Olympus viewer (download)

The fourth confocal is a Zeiss LSM510 NLO that is located in an interior lab (LSEB 450).

This systems currently is single photon only and schedules as Zeiss LSM510 (LSEB)

This system bills at a rate of $24.00 hr

SOP and information for the 510(coming soon)

Also located in an interior lab off of the confocal suite is LSEB 449 which house our JEOL JEM 2010 Transmission electron microscope.

This system schedules under the name JEOL 2010 TEM

Imaging related but not microscopes

LSEB 452-Wet lab for preparation of specimens.  NOTE: please provide your own consumables

This is also the location of the ultramicrotome for cutting epoxy sections

LSEB 448- This is home to our BD FACScalibur flow cytometer which is fine for cell analysis but unable to sort. ( For Sorting, please contact the BUMC Flow Cytometry Core Facility)

Our FACS is on the core scheduler as FACSCalibur (LSEB)FACS

This system has a service contract and its use is proportionally split.

SOP and information for FACScalibur(coming soon)

The departmental multimodal imager is a typhoon trio.  It is available on the scheduler as Typhoon Trio (LSEB)

Typhoon Trio

This system is currently not charged.

The Genepix 4000B is also housed in 448.GenePix

Users proportionately split the cost of repairs.

Finally, our fluorescent plate reader,  is available on the scheduler as Perkin Elmer Victor III (LSEB)

This system is currently not charged.