Imaging

The facility has numerous microscopes with different capabilities located throughout the Imaging suite and the department.

LSEB 448- Confocal imaging suite-

This suite currently houses 4 confocal microscope rigs, 2 data analysis stations as well as our transmission electron microscope*

*(The Jeol 2010 is offline and in need of some repairs that are not in the current budget.  If you have a funded project that needs TEM, we would LOVE to hear from you)

ALL OF THIS EQUIPMENT REQUIRES TRAINING PRIOR TO USE

The first bay (curtains at far left) houses an inverted Olympus IX70 inverted microscope with epi-fluorescence and a CCD camera. As well as the Nikon Eclipse system.

Image of the Nikon Eclipse workstation in LSE448 Bay#1The first bay of the imaging suite is home to shared widefield microscope (with epifluorescence) .  It is a Nikon Eclipse microscope with Nikon Elements Advanced research software for control and image analysis.  (NiE Specifications)

There is a nominal charge for use of $4.00/hr.  It is on the core scheduler as Nikon Eclipse NiE (LSEB).  All use must be recorded and some labs have priority of use.  If you are unfamiliar with this or unsure to whom to defer just ask.

The second set of curtains from the left is the Second bay with a Fluoview 300 confocal laser scanning microscope.  In the core scheduler, this is

In the core scheduler, this is Fluoview 1.

This system bills at a rate of $9.00 hr internal academic/$15.00 hr external academic

SOP and information for Fluoview 1

The third bay is home to an Olympus DSU spinning disk confocal.

In the core scheduler, it is Olympus DSU(LSEB)

This system bills at a rate of $9.00 hr internal academic/$15.00 hr external academic

SOP and information for DSU(coming soon)

The fourth bay is home to an Olympus Fluoview FV10i DOC inverted self-contained confocal laser scanning system.

It is excruciatingly popular and in the core scheduler it is FV10i

This system bills at a rate of $9.00 hr internal academic/$15.00 hr external academic

Data generated on the 10i can be analyzed with an offline Olympus viewer (download)

 

LSEB 449 (enter through LSE448)- Transmission Electron Microscope-

Located in an interior lab off of the confocal suite is LSEB 449 which houses our JEOL JEM 2010 Transmission electron microscope*.

When active this system schedules under the name JEOL 2010 TEM.

*(The Jeol 2010 is offline and in need of some repairs that are not in the current budget.  If you have a funded project that needs TEM, we would love to hear from you)

LSEB 450 (enter through LSE448)- Live Imaging Spectral Laser Scanning Microscope-

Located in an interior lab off of the main confocal suite is LSEB 450 which houses our new Nikon C2+ Si spectral laser scanning microscope. The system has an environmental chamber with gas, humidity and temperature control.
This system schedules under the name Nikon C2Si.  This system bills at a rate of $18.00 hr internal academic/$36.00 hr external academic.

Imaging related and support

LSE 448 outside bay 4-there is a full featured image analysis system running SlideBook 5 (intelligent imaging innovations) with full ratiometric and deconvolution modules for data analysis.  While the system is seamless for analyzing Olympus DSU data streams it will work with any data stream that is in a conventional image format.

LSEB 452-Wet lab for preparation of specimens

If you have a workflow and need lab space near the suite, we can arrange short and intermediate term accommodations.  After completion of any pilot studies, please provide your own consumables or provide an account number to the core to source supplies.

This is also the location of the ultramicrotome for cutting epoxy sections. The rotary microtome for cutting paraffin embedded samples, and the freezing stage microtome for cutting frozen tissues. There is also fume hood supporting histological staining workflows available upon arrangement.

 

LSEB 444 (enter through LSE452)

This is home to our BD FACScalibur flow cytometer which is fine for cell analysis but unable to sort. For Sorting, please contact the BUMC Flow Cytometry Core Facility

Our FACS analyzer is on the core scheduler as FACSCalibur (LSEB)Image of the FACSCalibur workstation in LSE448

This system bills at a rate of $25.00 per hour billed in half hours

SOP and information for FACScalibur

The departmental multimodal imager is a typhoon trio.  It is available on the scheduler as Typhoon Trio (LSEB)

Image of the Typhoon Trio workstation in LSE448

This system is currently not charged.

LSEB 632

Our cell sorter (SONY SH800) is located in LSE632 and samples are run by staff by appointment only. The cell sorter is $90/hr (in 15 minute increments).

 

Our fluorescent plate reader is available on the scheduler as Perkin Elmer Victor III (LSEB)

This can read fluorescence or luminescence in multiwell plate formats from above or below. It has conventional epifluorescence filters for GFP and RFP as well as DAPI.  This system is currently not charged but all use must be recorded.