Proteomics and Imaging Core Facility

The Proteomics and Imaging Core Facility is a multi-user core facility that while being part of the Biology Department serves the entire university.  The facility operates primarily as training and supervised usage facility rather than product on demand.   This is an overview of what we have and can do divided into three broad categories, Genomics, Proteomics and Imaging.  While there is a lot of overlap, it seems that this is the easiest way to subdivide.  All of our equipment requires initial training and where applicable is scheduled through the university core scheduling system.  Links to the scheduler and rates can be found on the instrument pages.

Click here for information on getting set up on the scheduler

Proteomics:

Our Proteomics section is currently more efficient with sample preparation but is a work in progress.  There is a wealth of information compiled originally by Dr John Tullai (thanks, John).

We have 2 mass spectrometers. An API 2000 MS/MS System (Manufacturer, Applied Biosystems/MDS Sciex, )

This instrument is a triple quadrupole mass spectrometer configured for small molecule analysis proteomics and is used for general training and collaborative projects.  It is especially useful for monitoring MRM transitions.

LCQ Advantage/ESI IT/MS ion trap mass spectrometer (Manufacturer, Thermo Finnigan,)

This instrument is configured for proteomic analysis of excised gel plugs via direct infusion.  It has an API stack and ESI source.

None of this equipment schedules online and it requires extensive training, or assisted operation.  Please contact us about any interest you have.

Imaging:

We have four rigs for Confocal Microscopy, a Transmission Electron Microscope, fluoresecent scanner, multi-mode fluorescent plate reader, slide scanner, and a BD FACSCalibur flow cytometer.

Genomics:

Our genomics instruments consist of real-time pcr cyclers, and a GenePix array scanner.