BUMC: Personal Protective Equipment

I. Purpose

This standard operating procedure (SOP) defines the requirements for personal protective equipment (PPE) and protective clothing.

Functions:

  • Protect personnel who enter animal facilities and/or perform animal procedures from exposure to animal dander, hair, secretions, and excretions that may cause allergic respiratory and skin responses, or that may cause illness.
  • Prevent exposure (to animal dander, hair, secretions, and excretions) of persons by contact with persons who have been in animal facilities or have conducted animal procedures.
  • Minimize the risk of possible ocular, oral, or dermal exposure to chemical disinfectants.
  • Minimize the transmission of disease agents among animals.
  • To protect personnel working with macaques or macaque tissues from the risk of Herpes B virus infection and other naturally occurring infectious diseases of macaques.

II. Scope/Responsibility

This procedure applies to anyone entering an animal or procedure room occupied by one or more animals.

III. Equipment/Materials

  • Scrub suit: This is clothing, typically referred to as “scrubs,” worn in place of street clothing when handling potentially contaminated, and/or mildly messy, materials to avoid contamination of street clothing. Scrubs are usually a lightweight, loose-fitting cotton shirt and pants. Typically, scrubs are provided and laundered by the institution.
  • Cloth lab coat: Cotton fabric coat that covers the torso and arms, usually worn over street clothing. Laboratory coats minimize contamination of clothing and skin beneath the gown by particulates and mists. Should be removed once contaminated.
  • Disposable lab coat: This is a lightweight fabric that covers the torso and arms, usually worn over scrubs or street clothing. Laboratory coats minimize contamination of clothing and skin beneath the gown by particulates and mists. Should be removed once contaminated.
  • Face mask: A variety of masks are used in the animal facilities. For the purpose of this SOP, a face mask refers to a surgical mask or cone mask.
  • Face mask and eye splash shield combination: This protects the user’s mouth and eyes from potential splashes or particulates.
  • Bouffant cap: This protects the wearer’s hair from particulate contamination and minimizes contamination of the environment by the wearer.
  • Disposable exam gloves: Gloves typically made of latex, nitrile, synthetic material, or vinyl, that protect the wearer’s hands from contamination. Exam gloves are important in minimizing contamination from animal to animal or from area to area, provided they are removed and replaced appropriately.
  • Shoe covers: Lightweight fabric or plastic booties worn over shoes to prevent contamination of the shoes and to prevent contamination of the environment by material that may be on the shoes. Shoe covers are especially important in minimizing the spread of contamination from area to area, provided they are removed and replaced appropriately.
  • Waterproof shoe covers: These are booties worn over shoes that are resistant to water.
  • Face shield: This is eye protection that provides splash protection from the sides and top. The top portion of the shield is composed of soft foam that rests against the wearer’s forehead, creating a protective barrier.
  • Safety glasses: Hard plastic eye protection worn to protect the wearer’s eyes from splashes and particulates.
  • Tyvek® or equivalent jumpsuit: This is made of a lightweight and water-resistant fabric that covers legs, torso, and arms.
  • Sleeve covers: These are lightweight, water-resistant fabric covers for the lower arms.
  • Rubber boots: These are waterproof boots made from lightweight rubber.
  • N-95 respirator: This is a respirator whose filtering efficiency has been determined to be at least 95% for the most penetrating sized particle (~0.3 um). These minimize exposure to rodent dander and allergens. They are available to those who choose to use them. Respiratory fit testing by EHS is required for use.

IV. Procedures

A. ALL ANIMAL AREAS
The following must be worn when entering the animal corridors on W8, R8, and M9:

  • Cloth lab coat

The following must be worn when entering the animal corridors on W7 and W9:

  • Disposable lab coat
  • Shoe covers

These items must be removed as you exit across the threshold demarcated by the checkered strip on the floor.

B. RODENT ANIMAL AREAS
The following must be worn to enter the W9 Barrier Area rodent housing rooms:

  • Disposable lab coat
  • Face shield or safety glasses
  • Face mask (or face mask and face shield combination)
  • Shoe covers
  • Gloves—these must be changed between racks (if handling animals)
  • Sleeve covers (if handling animals)—these must be changed between racks
  • Bouffant cap

The following must be worn to enter the W9 Barrier Area rodent procedure rooms:

  • Disposable lab coat
  • Shoe covers
  • Bouffant cap
  • Face shield or safety glasses (if handling animals)
  • Face mask (if handling animals)
  • Gloves (if handling animals)
  • Sleeve covers (if handling animals)
  • The following must be worn to enter W8, R8, and M9 rodent housing rooms:
  • Cloth lab coat
  • Face shield or safety glasses
  • Face mask (or face mask and face shield combination)
  • Gloves—these must be changed between racks (if handling animals)
  • Sleeve covers (if handling animals)—these must be changed between racks
  • The following must be worn to enter any rodent procedure rooms:
  • Cloth lab coat
  • Face shield or safety glasses (if handling animals)
  • Face mask (if handling animals)
  • Gloves (if handling animals)
  • Sleeve covers (if handling animals)
  • The following must be worn to enter the 670 Albany Street Rodent Barrier Facility:
  • Surgical scrub suit
  • Coveralls
  • Shoe covers
  • Bouffant cap
  • Gloves

The following must be worn to enter the 670 Albany Street Rodent Barrier Facility animal housing rooms in addition to items 5a-e:

  • Face shield or safety glasses
  • Face mask
  • Sleeve covers (if handling animals)

C. MACAQUE ANIMAL AREAS
The following must be worn by LASC staff or research staff to enter a housing room or hold a macaque:

  • Scrubs and a disposable lab coat or Tyvek or equivalent jumpsuit (if wearing street clothes)
  • Bouffant cap
  • Waterproof shoe covers—shoe covers are removed upon leaving the room
  • Face shield (the mask-face shield combination is not acceptable). The face shield must provide splash protection from the sides and top.
  • Face mask
  • Two pairs of gloves
  • Sleeve covers

The following must be worn by LASC staff during a cage change in a macaque holding room:

  • Scrubs and a disposable Tyvek or equivalent jumpsuit
  • Bouffant cap
  • Waterproof shoe covers—shoe covers are removed upon leaving the room
  • Face shield (the mask-face shield combination is not acceptable). The face shield must provide splash protection from the sides and top.
  • Face mask
  • Two pairs of gloves
  • Sleeve covers

D. CAT/CHINCHILLA/RABBIT/PIG ANIMAL AREAS
The following must be worn to enter a room housing or holding a cat/chinchilla/rabbit/pig or a procedure room to handle or observe the animals:

  • Cloth or disposable lab coat
  • Shoe covers (not required in cat areas)
  • Gloves

The following must be worn to enter a room housing a cat/chinchilla/rabbit/pig to perform physical work (such as washing pens):

  • Disposable lab coat
  • Waterproof shoe covers/rubber boots (not required in cat areas)
  • Gloves
  • Face shield

E. CAGE WASH AREAS
The following must be worn to enter the clean side of the cage wash:

  • Scrubs
  • Disposable lab coat
  • Bouffant cap
  • Shoe covers
  • Face shield/safety glasses
  • Gloves

The following must be worn to enter the dirty side of the cage wash:

  • Scrubs
  • Disposable lab coat
  • Bouffant cap
  • Face shield/safety glasses
  • Face mask (or face mask and splash shield combination)
  • Rubber boots/waterproof shoe covers
  • Gloves