BME PhD Prospectus Defense - Shannon Tunney

  • Starts: 10:00 am on Tuesday, December 4, 2018

Title: “Construction of Molecular Tools through Post-Translation Protein Excision and Splicing”

Committee: John Ngo, PhD – BU BME (Advisor, Chair) Mary Dunlop, PhD – BU BME Allyson Sgro, PhD – BU BME Trevor Siggers, PhD – BU Biology

Abstract: Advances in analysis of protein structure have created a library of parts for synthetic biologists to use in non-native contexts to adapt natural functions for biotechnologies such as therapeutics. However, to achieve complex function, these pieces are often cleaved or combined post-translationally. Additionally, the common systems used for the purpose originate from mammalian systems, making them risky to use in human medicine. This work explores the use of drug-inhibitable viral protease and yeast splicing enzymes to post-translationally control protein domain association. Viral protease and small molecule inhibitors are used in a system for controlling the location of a transcription factor in a cell, and thereby its activity. Both protease and splicing enzymes are used in a system to control the strength of a transcription factor with high sensitivity to drug input and finally, in a system to confer tight control of protein activity under the regulation of three different inputs.

48 Cummington Mall (ERA), room B11

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