BME PhD Prospectus Defense - Jiaxi Jessie Song

  • Starts: 9:00 am on Tuesday, September 25, 2018
Title: "Single-Molecule Protein Identification Using Electro-Optical Sensing in Solid-State Nanopores" Committee: Prof. Mark W. Grinstaff – BU BME (Advisor, Chair) Prof. Amit Meller – BU BME, Technion Israel Institute of Technology Prof. John T. Ngo – BU BME Dr. Nancy S. Miller – BU School of Medicine Abstract: Proteins are vital and ubiquitous in biological systems. Being able to detect and identify proteins provides valuable information for the diagnosis and treatment of various diseases. Here I describe a highly sensitive nanopore-based protein identification method whereby a protein conjugated with three types of fluorophores respectively to lysine, cysteine, and the N-terminal amine translocate through a sub 5 nm opti-nanopore. During translocation, both the electrical and optical signals across the nanopore are recorded. Since the majority of proteins have their unique fingerprints of lysine and cysteine residues in their sequences, the success of this project will enable researchers to identify most proteins with high precision rate via reference to the human proteome database. Accordingly, in the first objective, I will optimize the labeling chemistry so that the lysine, cysteine, and N-terminal amine will be labeled with three different fluorophores in a 45-mer polypeptide. The labeled peptides will then be tested in the second objective to obtain the electro-optical signals as the molecules translocate through a solid-state opti-nanopore. Lastly, in the third objective, I will apply the optimized labeling conditions and detection parameters in the first two objectives to identify biologically relevant peptides and proteins using opti-nanopores. Completion of these objectives will lead to wider biomedical applications such as identifying unknown protein biomarkers that are unable to be resolved with conventional protein identification techniques.
44 Cummington Mall, Room 203

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