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Protocols

ES Cells | iPS Cells | Vectors

 

Embryonic Stem Cell Culture Protocols

  1. Mouse ESC Media with Serum
  2. Serum free ESC maintenance media
  3. ESC Passage
  4. Thawing and Freezing ES Cells
  5. Transduction of mESC with Lentivirus
  6. Making Mouse Embryonic Fibroblasts (MEFs)
  7. Shipping Tail Tips
  8. Mouse ESC differentiation to Nkx2-1+ lung and thyroid progenitors (Bio-Protocol)
  9. 2i Media (Feeder Free, Serum Free)

 

iPS Cell Culture Protocols

  1. Human Dermal Fibroblast Isolation
  2. Shipping Live Culture Fibroblasts
  3. Isolation of Peripheral Blood Mononuclear Cells for Reprogramming
  4. Mouse iPS Cell Generation with STEMCCA
  5. Human iPS Cell Generation with hSTEMCCA
  6. Human iPS Cell Generation from Peripheral Blood with hSTEMCCA
  7. Southern Blot with human gDNA to screen for hSTEMCCA integration in hiPS cells

 

Vector Protocols

  1. Protocol 1: Lentivirus Packaging by 293T Transfection
  2. Protocol 2: Calculations for Transfection of 293T for Lenti/Retro Packaging
  3. Protocol 3: Titering Lentivirus on FG293 Cells
  4. Protocol 4: Titering Lentiviral Vectors by Southern Blot
  5. Protocol 5: Retrovirus Packaging
  6. Protocol 6: Primer Design for Cloning Genes into the pHAGE Lentiviral Backbone
  7. Protocol 7: Bacterial Storage and Glycerol Stocks
  8. Protocol 8: Lentiviral Transduction of Hematopoietic Stem Cells
  9. Protocol 9: Lentiviral Transduction of Alveolar Macrophages by Intratracheal Injection

**The Boston University Center for Regenerative Medicine endorses the International Stem Cell Registry (U.Mass), and The National Stem Cell bank (WiCell; University of Wisconsin). Detailed human ES Culture protocols and cell banks are maintained at each of these sites; click below to access their highly recommended protocol pages:

  1. International Stem Cell Registry
  2. National Stem Cell Bank; WiCell