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Basic iPSC Hands-On Training Course

The CReM iPSC Core is offering basic training in human induced pluripotent stem cell (hiPSC) culture for research investigators working with or planning to work with hiPS cells. The hands-on training will take place over 2 weeks (approximately 1 hour per day) and will provide investigators with the techniques necessary to maintain and propagate established iPS cells in their own lab. Training will focus on feeder-free (mTeSR1/Matrigel culture system) hiPS cell culture methods including colony passaging using common cell dissociation reagents and mechanical picking, identification of differentiated cells, cryopreservation and thawing. Detailed SOPs will be provided including methods for characterizing iPSCs for karytoype analysis and for pluripotency by immunofluorescent staining. Prior experience in general cell culture techniques is required.

  1. Please contact Marianne James for further information ( mfjames@bu.edu ). Cost of Training Course: $1200/BU; $1500/non-BU