Irina V. Zhdanova, MD, PhD
Drs. Friedman and Yesavage; Stanford University
Dr. Kishi; Dana Farber Cancer Institute, Harvard University
Dr. O'Malley; Northeastern University
Dr. Balis and McCully; NIH/NCI
Laboratory of Sleep and Circadian Physiology

Primates
Telemetric polysomnographic (EEG, EOG, EMG) recordings of sleep patterns and core body temperature are conducted in specially designed primate sleep lab, which includes six sound and light insulated 'sleep chambers'.

Continuous recordings of locomotor activity, food intake and cognitive performance in monkeys that receive their food by operating individual touch screen computer monitors build into their home cages.

Zebrafish
High throughput locomotor activity assay, using image analysis system allows us to study the sleep-like state and circadian rhythms in this diurnal vertebrate. Each of our four systems allows to record up to 160 larval or 30 adult fish at a time.

High speed video camera recordings (1000 frames per second) document detailed locomotor patterns in larval and adult zebrafish.

Confocal calcium imaging of neuronal activity in behaving zebrafish helps in our search of the specific neuronal structures involved in the behaviors of interest (collaboration with Dr. O'Malley; Northeastern University)

Construction of reporter genes using the Green Fluosrescent Protein (GFP) helps to record their temporal/spatial expression and regulation in the zebrafish

In vivo inhibition of protein expression by anti-sense RNAs allows to manipulate genes of interest and to study their contribution to development and behavior.

General Methods
Immunoassays for melatonin, cortisol, cAMP and cGMP in human and animal body fluids and tissues.

Analysis of protein expression by Western analysis

Laboratory of Autism Neuroscience Research
Laboratory of Brain Imaging
Laboratory of Cardiovascular Biology
Laboratory of Cerebral Dynamics
Cellular Biology of the Basal Ganglia and Motor Disorders.
Laboratory of Cognitive Neurobiology
Laboratory of Developmental Cognititve Neuroscience
Laboratory of Electron Microscopy
Laboratory of In Vitro Neurophysiology
Laboratory of Systems Molecular Signaling and Chemical Biology
Laboratory of Neuropsychology
Laboratory of Retinal Microcircuitry
Laboratory of Sleep and Circadian Physiology
Laboratory of Stereology and Morphometry
Laboratory of Visual Neuropathology
Laboratory of Visual Perception and Cognition