BU-CTSI support for Affymetrix microarray or RNA sequencing experiments
Release Date: February 12, 2018
Application Deadline: Closed
PURPOSE OF THIS RFA
To support translational research through
- Subsidies that cover part of the cost of mRNA or miRNA microarrays in the BU Microarray and Sequencing Resource
- Subsidies that cover part of the cost of RNA sequencing projects in the BU Microarray and Sequencing Resource
Full-time BU faculty. (Graduate students or post-doctoral fellows should apply under the sponsorship of their full-time BU faculty mentor.)
- Subsidies for microarrays: These will cover part of the cost of mRNA or miRNA microarray laboratory supplies and processing, along with basic gene-level analysis, in the BU Microarray and Sequencing Resource (BUMSR) (Director, Yuriy Alekseyev, PhD). For projects that are chosen for support, BU-CTSI will cover material and processing costs up to a total of $6,000.00 per project. (Note: It is expected that RNA extraction from samples will be performed by the investigator, and the cost of this is not covered. The investigators will also be charged $110 per sample, which includes $10 for QC). The arrays may be from any of the Gene 1.0 ST or 2.0 ST, Clariom S, or miRNA v3.0 or v4.0 platforms.
- Subsidies for RNA sequencing: These will cover part of the cost of poly-A RNA (not total RNA) sequencing laboratory supplies and processing, along with basic gene-level analysis, in the BUMSR. For projects that are chosen for support, BU-CTSI will cover material and processing costs up to a total of $6,000.00 per project. (Note: It is expected that RNA extraction from samples will be performed by the investigator, and the cost of this is not covered. The investigators will also be charged $110 per sample, which includes $10 for QC). All projects will be sequenced using the Illumina NextSeq 500 instrument. Investigators can choose either single-end or paired-end 75-base reads, but projects must consist of multiples of 6 samples to facilitate optimal usage of the instrument.
Because all laboratory work subsidized by this program must be completed by March 31, 2018, investigators should only apply for support if their research timeline is such that the core laboratory use or delivery of RNA specimens to the BUMSR can take place within this time frame. This is of particular importance for sequencing projects, which are performed on batches of 12 samples; prompt receipt of RNA samples for 6- or 18-sample projects will therefore prevent delays in putting together a full sequencing run.
Only projects that have all necessary IRB, IACUC, and IBC approvals already completed are eligible (approval numbers and expiration dates required in the application).
By accepting this support, the applicant agrees to submit a brief progress report to BU-CTSI indicating key results and any publications, grant applications, grants, or intellectual property that resulted from the project. This brief progress report will be due approximately one year after completion of the funded work is conducted. The applicant also agrees to cite BU-CTSI support in resultant publications.
REVIEW PROCESS AND REVIEW CRITERIA
Applications will be reviewed by the leadership of BU-CTSI, and decisions whether to support a request will take into consideration:
- Significance of the work in terms of potential for translation and impact on disease prevention, diagnosis, or treatment
- Scientific rigor and novelty of the proposed approach. Proposals must include an appropriate sample size to achieve statistical power (a minimum of three biological replicates per group is strongly recommended)
- Likelihood that the project will lead to subsequent external funding and/or commercial development
Funded projects must adhere as closely to the proposed experimental design as possible. Deviations from the proposed design (due to insufficient sample material, etc.) will be permitted within reason, subject to the final decision of the CTSI and the BUMSR staff.
WHERE TO SEND INQUIRIES
We encourage inquiries concerning this RFA and welcome the opportunity to answer questions from potential applicants. Direct your questions to:
|Adam Gower, PhD
Clinical & Translational Science Institute
Boston University Microarray and Sequencing Resource
Boston University Clinical and Translational Science Institute
We are pleased to announce that the following recipients have been selected to receive support for a portion of the labor, reagent, and sample processing costs for their proposed projects.
|Center for Regenerative Medicine|
|Pharmacology & Experimental Therapeutics|
|Pathology & Laboratory Medicine|
|Marcello Panagia||Cardiovascular Medicine|
|Makoto Senoo||GSDM Molecular & Cell Biology|
|Lukasz Stawski||Arthritis Center|
|Xiaoling Zhang||Genetics, Molecular Medicine|